Estrogen Induces Expression of Endometrial Epidermal Growth Factor Receptors Before Implantation

Kleinstein, J.; Westermann, W.; Mennenga, Kirsten; Gustmann, Ch.; Gips, H.

doi:10.1111/j.1600-0897.1993.tb00602.x

Cited by 7 publications

(2 citation statements)

References 13 publications

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“…EGF and c-fos are important factors in the uterine mitogenic and weight responses to estrogen (Gonzalez et al, 1984;David et al, 1988;Weisz and Bresciani, 1988;Halling and Forsberg, 1993;Kleinstein et al, 1993). Both the uterine EGF receptor and expression of the EGF gene are under estrogen control (Mukku and Stancel, 1985;Lingham et al, 1988;Huet-Hudson et al, 1990;Astroff et al, 1990;Kleinstein et al, 1993).…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical studies on the expression and estrogen dependency of EGF and its receptor and C-fos proto-oncogene in the uterus and vagina of normal and neonatally estrogen-treated mice

Falck

Forsberg

1996

Anat. Rec.

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Background The final target cell response to estrogen is dependent not only on the estrogen receptor, but also on autocrine/paracrine interactions with growth factors (e.g., EGF) and proto‐oncogenes (e.g., c‐fos). Because neonatal estrogen treatment results in permanent changes in the female mouse genital tract (permanent vaginal cornification, cervical adenosis and tumors, changed growth control mechanisms in uterus), it was of interest to study possible acute and permanent effects of such treatment on distribution and levels of EGF, its receptor (EGF‐r), and c‐fos and to relate such changes to morphological development and appearance of epithelial abnormalities. Methods Immunohistochemical techniques using frozen sections from the uterus and vagina of neonatal and adult (ovariectomized, estradiol‐treated) females, treated with olive oil or diethylstilbestrol in neonatal life. Results A difference in stromal‐epithelial distribution of EGF was demonstrated with respect to region studied (uterus, vagina) and age (neonatal, adult). EGF was localized mainly in the uterine stroma but in both vaginal epithelium and stroma (with a different pattern compared to uterus). In neonatal females, EGF occurred in both tissue components in both regions, and the distribution pattern was quite different from that in adult females. The EGF level was increased by estrogen in adult but not in neonatal females. EGF‐r and c‐fos occurred in both uterine epithelium and stroma and in the vaginal epithelium; levels and distribution pattern were affected by estrogen. Neonatal estrogen treatment increased the levels of uterine EGF and c‐fos in adult life. Conclusions There are distinct developmental changes in the distribution and estrogen sensitivity of EGF. Only further studies can prove or disprove the association between the earlier reported disturbed growth control mechanisms in the uterus of adult but neonatally estrogen‐treated females and the increased levels of uterine EGF and c‐fos. The present results do not seem to explain mechanisms involved in the origin of neonatally estrogen‐induced cervicovaginal epithelial abnormalities, nor do they explain the earlier described difference in estrogen‐induced proliferative response between the uterine cervix and uterus proper. © 1996 Wiley‐Liss, Inc.

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Section: Discussionmentioning

confidence: 99%

Immunohistochemical studies on the expression and estrogen dependency of EGF and its receptor and C-fos proto-oncogene in the uterus and vagina of normal and neonatally estrogen-treated mice

Falck

Forsberg

1996

Anat. Rec.

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“…Prentice et al (1992) and Chegini et al (1992) failed to demonstrate any cycle-dependent immunohistochemical staining of EGF receptors. In the rabbit Kleinstein et al (1993) showed that an increase of endometrial EGF binding was dependent only on the exposure to oestrogens, whereas progesterone showed no significant effect.…”

Section: Discussionmentioning

confidence: 95%

Flow cytometric detection and binding studies of human endometrial stromal cell epidermal growth factor receptor in monolayer culture: influence of progesterone

Singer¹,

Strowitzki²,

Rettig³

1998

Molecular Human Reproduction

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Epidermal growth factor (EGF) has been shown to modulate endometrial differentiation in vivo and in vitro. Therefore, endometrial stromal cell EGF receptors were characterized in intact endometrial stromal cells, cultured in vitro. The methods used for characterization were flow cytometry, binding and displacement studies, and gel electrophoresis followed by autoradiography. In flow cytometry the histogram of labelled stromal cells was identical to Caski cells, which served as a positive control. EGF binding revealed the typical binding hierarchy for EGF receptors. The Scatchard analysis showed a curvilinear plot with a calculated dissociation constant of 0.36 nM for high affinity binding sites. In autoradiography a band of approximately 170 kDa was visualized corresponding to the known size of the EGF receptor. The intensity of this band was increased by pretreatment of stromal cells with 10 nM progesterone for 4 days. Furthermore, stimulation with progesterone led to an increase in specific EGF binding activity of stromal cells by 21% compared to control. These data indicate that intact stromal cells in monolayer culture maintain specific EGF receptors, which are up-regulated by progesterone.

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Role of Growth Factors of Uterine and Fetal-Placental Origin During Pregnancy

Rider¹,

Piva²

1998

Endocrinology of Pregnancy

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Estrogen Induces Expression of Endometrial Epidermal Growth Factor Receptors Before Implantation

Abstract: These results provide insight into the regulation of the autocrine and paracrine factor EGF in the endometrium during the preimplantation period.

Cited by 7 publications

References 13 publications

Immunohistochemical studies on the expression and estrogen dependency of EGF and its receptor and C-fos proto-oncogene in the uterus and vagina of normal and neonatally estrogen-treated mice

Immunohistochemical studies on the expression and estrogen dependency of EGF and its receptor and C-fos proto-oncogene in the uterus and vagina of normal and neonatally estrogen-treated mice

Flow cytometric detection and binding studies of human endometrial stromal cell epidermal growth factor receptor in monolayer culture: influence of progesterone

Role of Growth Factors of Uterine and Fetal-Placental Origin During Pregnancy

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