Estrogen induces stromal cell-derived factor 1 (SDF-1/CXCL12) production in human endometrial stromal cells: a possible role of endometrial epithelial cell growth

Tsutsumi, Atsushi; Okada, Hidetaka; Nakamoto, Tetsuji; Okamoto, Rei; Yasuda, Katsuhiko; Kanzaki, Hideharu

doi:10.1016/j.fertnstert.2010.08.037

Cited by 42 publications

(42 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Furthermore, studies with PR-deficient mice have demonstrated that P is unable to decrease the E 2 -induced proliferative effect on the endometrium (36). Regarding endometrial growth, in a previous study we had found that SDF-1 is a candidate factor involved in the cross-talk of the epithelial-stromal interaction of the human endometrium and may be actively involved in its proliferation (14). E 2 may have effects on angiogenesis and growth in the human endometrium via a synergistic pathway involving both VEGF and SDF-1.…”

Section: Discussionmentioning

confidence: 95%

“…The supernatant was collected after stimulation and stored at À80 C until assayed. The number of ESCs was counted by the trypan blue method as described previously (14).…”

Section: Figurementioning

confidence: 99%

“…Several recent studies have suggested the importance of SDF-1 in human endometrial function. SDF-1 may play crucial roles in the endometrial proliferation, embryo implantation, and leukocyte recruitment to the endometrium (12)(13)(14). VEGF and SDF-1 interact synergistically to promote the functions of vascular endothelial cells, such as cell migration, cell survival, and gene expression (9,11,15).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Progestins inhibit estradiol-induced vascular endothelial growth factor and stromal cell–derived factor 1 in human endometrial stromal cells

Okada

Okamoto

Tsuzuki

et al. 2011

Fertility and Sterility

Self Cite

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 95%

“…The supernatant was collected after stimulation and stored at À80 C until assayed. The number of ESCs was counted by the trypan blue method as described previously (14).…”

Section: Figurementioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Progestins inhibit estradiol-induced vascular endothelial growth factor and stromal cell–derived factor 1 in human endometrial stromal cells

Okada

Okamoto

Tsuzuki

et al. 2011

Fertility and Sterility

Self Cite

View full text Add to dashboard Cite

“…Loss of P 4 signaling in the endometrium may be a causal factor in the development of EMS, and P 4 resistance is commonly observed in women with this disease (Bulun et al 2010). Tsutsumi et al (2011) reported that medroxyprogesterone acetate almost completely antagonized the E 2 -induced CXCL12 production in normal ESCs. In our study, E 2 stimulated CXCL12 production from normal and ectopic ESCs in a time-dependent manner.…”

Section: Discussionmentioning

confidence: 99%

“…Estradiol (E 2 ) enhanced CXCL12 production levels in human endometrial stromal cells (ESCs) in a time-and dosedependent manner (Tsutsumi et al 2011). Progestins effectively inhibit estrogen-induced CXCL12 expression in human ESCs .…”

Section: Introductionmentioning

confidence: 99%

The role of SRC1 and SRC2 in steroid-induced SDF1 expression in normal and ectopic endometrium

Shi

Dai

et al. 2014

Reproduction

View full text Add to dashboard Cite

To compare the expression patterns of steroid receptor coactivators (SRCs) and steroid-induced stromal cell-derived factor 1 (CXCL12 (SDF1)) in normal and ectopic endometrium and to explore the roles of NCOA1 (SRC1) and NCOA2 (SRC2) in the steroid-induced CXCL12 expression in normal and ectopic endometrial stromal cells (ESCs). The NCOA1, NCOA2, NCOA3 (SRC3), and CXCL12 (SDF1)a mRNA levels in normal and ectopic endometrium were analyzed by quantitative real-time PCR. Steroid-induced CXCL12 expression was detected by the ELISA method and the chemotactic activity of conditioned supernatant to monocyte was assessed by the Boyden chamber method before and after the silencing of NCOA1 or NCOA2 with siRNA in normal and ectopic ESCs. The expression of NCOA1 and CXCL12 in ectopic endometrium was significantly greater than that in normal endometrium in the secretory phase. Progesterone (P 4 ) was able to significantly inhibit estradiol (E 2 )-stimulated CXCL12 expression in normal and ectopic ESCs. The inhibitory rate of P 4 in ectopic ESCs at 72 and 96 h was significantly lower than that in normal ESCs. Silencing of NCOA1 but not NCOA2 significantly reduced the E 2 -induced CXCL12 expression in normal and ectopic ESCs. The ability of P 4 to inhibit E 2 -induced CXCL12 expression and monocyte chemotaxis in normal and ectopic ESCs was significantly attenuated when NCOA2 was silenced. NCOA1 plays a necessary role in E 2 -induced CXCL12 expression and NCOA2 is required for P 4 to inhibit the E 2 -induced CXCL12 production in normal and ectopic endometrium.

show abstract

Angiogenesis mediators in women with idiopathic heavy menstrual bleeding

Elkilani

Soliman

2016

Intl J Gynecology & Obste

View full text Add to dashboard Cite

show abstract

Estrogen induces stromal cell-derived factor 1 (SDF-1/CXCL12) production in human endometrial stromal cells: a possible role of endometrial epithelial cell growth

Cited by 42 publications

References 13 publications

Progestins inhibit estradiol-induced vascular endothelial growth factor and stromal cell–derived factor 1 in human endometrial stromal cells

Progestins inhibit estradiol-induced vascular endothelial growth factor and stromal cell–derived factor 1 in human endometrial stromal cells

The role of SRC1 and SRC2 in steroid-induced SDF1 expression in normal and ectopic endometrium

Angiogenesis mediators in women with idiopathic heavy menstrual bleeding

Contact Info

Product

Resources

About