Estrogen-Like Effect of Bazi Bushen Capsule in Ovariectomized Rats

Wang, Dandong; Chang, Liping; Hao, Jiameng; Zhang, Jiehan; Song, Ye‐Qiong; Ma, Kai-Kuang; Zhang, Shaolan; Li, Cuiru; Gu, Jiaojiao; Hou, Yunlong; Wu, Yi‐Ling

doi:10.3791/64884

Cited by 2 publications

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“…Prescription tonic of ‘ kidney essence’ balances ‘ Yin and Yang ’ and enhances archaeus, making the body healthy and full of spirit. Previous studies have shown that BZBS has anti-ageing effects [ [14] , [15] , [16] , [17] , [18] , [19] ], such as inhibiting premature senescence in mice, slowing methylation, and maintaining telomere length. Furthermore, it is believed that the ‘ kidney essence ’ is closely related to stem cells.…”

Section: Introductionmentioning

confidence: 99%

Mechanism of Bazi Bushen capsule in delaying the senescence of mesenchymal stem cells based on network pharmacology and experimental validation

Zhang,

Wang,

Song

et al. 2024

Heliyon

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Section: Introductionmentioning

confidence: 99%

Mechanism of Bazi Bushen capsule in delaying the senescence of mesenchymal stem cells based on network pharmacology and experimental validation

Zhang,

Wang,

Song

et al. 2024

Heliyon

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“…BZBS has been shown to mitigate epigenetic aging and extend health span in naturally aging mice ( Mao et al, 2023 ). Furthermore, BZBS has demonstrated beneficial effects on aging-related diseases in various organs, especially in improving cognitive function ( Ji et al, 2022 ; Wang et al, 2023 ). However, the potential of BZBS to delay aging by repairing intestinal damage caused by accelerated aging has not been explored yet.…”

Section: Introductionmentioning

confidence: 99%

Bazi Bushen capsule improves the deterioration of the intestinal barrier function by inhibiting NLRP3 inflammasome-mediated pyroptosis through microbiota-gut-brain axis

Zhang,

Li,

Chang

et al. 2024

Front. Microbiol.

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PurposeThe senescence-accelerated prone mouse 8 (SAMP8) is a widely used model for accelerating aging, especially in central aging. Mounting evidence indicates that the microbiota-gut-brain axis may be involved in the pathogenesis and progression of central aging-related diseases. This study aims to investigate whether Bazi Bushen capsule (BZBS) attenuates the deterioration of the intestinal function in the central aging animal model.MethodsIn our study, the SAMP8 mice were randomly divided into the model group, the BZ-low group (0.5 g/kg/d BZBS), the BZ-high group (1 g/kg/d BZBS) and the RAPA group (2 mg/kg/d rapamycin). Age-matched SAMR1 mice were used as the control group. Next, cognitive function was detected through Nissl staining and two-photon microscopy. The gut microbiota composition of fecal samples was analyzed by 16S rRNA gene sequencing. The Ileum tissue morphology was observed by hematoxylin and eosin staining, and the intestinal barrier function was observed by immunofluorescence. The expression of senescence-associated secretory phenotype (SASP) factors, including P53, TNF-α, NF-κB, IL-4, IL-6, and IL-10 was measured by real-time quantitative PCR. Macrophage infiltration and the proliferation and differentiation of intestinal cells were assessed by immunohistochemistry. We also detected the inflammasome and pyroptosis levels in ileum tissue by western blotting.ResultsBZBS improved the cognitive function and neuronal density of SAMP8 mice. BZBS also restored the intestinal villus structure and barrier function, which were damaged in SAMP8 mice. BZBS reduced the expression of SASP factors and the infiltration of macrophages in the ileum tissues, indicating a lower level of inflammation. BZBS enhanced the proliferation and differentiation of intestinal cells, which are essential for maintaining intestinal homeostasis. BZBS modulated the gut microbiota composition, by which BZBS inhibited the activation of inflammasomes and pyroptosis in the intestine.ConclusionBZBS could restore the dysbiosis of the gut microbiota and prevent the deterioration of intestinal barrier function by inhibiting NLRP3 inflammasome-mediated pyroptosis. These results suggested that BZBS attenuated the cognitive aging of SAMP8 mice, at least partially, by targeting the microbiota-gut-brain axis.

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Estrogen-Like Effect of Bazi Bushen Capsule in Ovariectomized Rats

Cited by 2 publications

References 0 publications

Mechanism of Bazi Bushen capsule in delaying the senescence of mesenchymal stem cells based on network pharmacology and experimental validation

Mechanism of Bazi Bushen capsule in delaying the senescence of mesenchymal stem cells based on network pharmacology and experimental validation

Bazi Bushen capsule improves the deterioration of the intestinal barrier function by inhibiting NLRP3 inflammasome-mediated pyroptosis through microbiota-gut-brain axis

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