Estrogen modulates osteoblast proliferation and function regulated by parathyroid hormone in osteoblastic SaOS-2 cells: role of insulin-like growth factor (IGF)-I and IGF-binding protein-5

Nasu, M; Sugimoto, Toshitsugu; Kaji, Hidesuki; Chihara, Kazuo

doi:10.1677/joe.0.1670305

Cited by 37 publications

(26 citation statements)

References 43 publications

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“…However, male mice in the absence of PTH tended to have more osteoprogenitor cells and higher ALP activities than female mice, and this difference persisted following PTH administration. In a previous study, Nasu et al(2000) demonstrated that PTH stimulated osteoblast function such as ALP activity and collagen synthesis following pretreatment with estrogen, but it did not affect osteoblast function in the absence of estrogen P<0·05 control vs PTH treatment; F, female; M, Male; TV, total bone volume (mm pretreatment, suggesting that estrogen plays an important role in the anabolic effects of PTH on osteoblast differentiation. This suggests that the gender difference in the anabolic effects of PTH on BMSC is at least partly based on estrogen action.…”

Section: Discussionmentioning

confidence: 94%

“…In this study, we found that PTH increased the mRNA levels of IGF-I and its receptor (IGF-IR) only in male mice. Although several reports suggested a stimulatory effect by estrogen on IGF-I synthesis by osteoblasts (Watson et al 1995, Nasu et al 2000, recent findings indicate that the influence of estrogen on IGF-I gene expression is complex and perhaps indirect (McCarthy et al 1997). Estrogen is able to diminish IGF-I gene activation by PTH or PGE2 as well as by GH (McCarthy & Centrella 2001).…”

Section: Discussionmentioning

confidence: 98%

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Gender differences in the response of CD-1 mouse bone to parathyroid hormone: potential role of IGF-I

Wang

Sakata²,

Elalieh³

et al. 2006

Journal of Endocrinology

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Parathyroid hormone (PTH) exerts both catabolic and anabolic actions on bone. Studies on the skeletal effects of PTH have seldom considered the effects of gender. Our study was designed to determine whether the response of mouse bone to PTH differed according to sex. As a first step, we analyzed gender differences with respect to bone mass and structural properties of 4 month old PTH treated (80 µg/kg per day for 2 weeks) male and female CD-1 mice. PTH significantly increased fat free weight/body weight, periosteal bone formation rate, mineral apposition rate, and endosteal single labeling surface, while significantly decreasing medullary area in male mice compared with vehicle treated controls, but induced no significant changes in female mice. We then analyzed the gender differences in bone marrow stromal cells (BMSC) isolated from 4 month old male and female CD-1 mice following treatment with PTH (80 µg/kg per day for 2 weeks). PTH significantly increased the osteogenic colony number and the alkaline phosphatase (ALP) activity (ALP/cell) by day 14 in cultures of BMSCs from male and female mice. PTH also increased the mRNA level of receptor activator of nuclear factor B ligand in the bone tissue (marrow removed) of both females and males. However, PTH increased the mRNA levels of IGF-I and IGF-IR only in the bones of male mice. Our results indicate that on balance a 2-weeks course of PTH is anabolic on cortical bone in this mouse strain. These effects are more evident in the male mouse. These differences between male and female mice may reflect the greater response to PTH of IGF-I and IGF-IR gene expression in males enhancing the anabolic effect on cortical bone.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 98%

Gender differences in the response of CD-1 mouse bone to parathyroid hormone: potential role of IGF-I

Wang

Sakata²,

Elalieh³

et al. 2006

Journal of Endocrinology

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“…Part of these differences is most likely due to the sex steroid environment [15, 16, 17]. In addition, the higher expression of IGFBP-5 in females during pubertal development may be directly related to their higher levels of estrogens, since in some tissues IGFBP-5 expression is stimulated by estradiol [18]. …”

Section: Discussionmentioning

confidence: 99%

Inverse Correlation between Insulin-Like Growth Factor (IGF)-Binding Protein-5 and IGF-I and II during Postnatal Development of the Anterior Pituitary Gland

et al. 2002

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Background: The insulin-like growth factor (IGF) system is important for pituitary development and control, with each member of this axis having a specific temporal and spatial expression. Because IGF-binding protein-5 (IGFBP-5) is one of the most highly expressed binding proteins in the anterior pituitary (AP), it might be of special importance in this gland. Objective: The purpose of this study was to examine the temporal relationship between the expression of the IGFs and IGFBP-5 in the AP during postnatal development. Design and Methods: Quantitative reverse transcription polymerase chain reaction was used to study the mRNA levels of these proteins in the AP of male and female rats at 10, 20, 30, 40 and 60 days of age. Results: The highest mRNA levels of IGF-I and II occurred at 10 and 20 days of age with a dramatic decrease at 30 days (p < 0.0001). IGF-I, but not IGF-II, mRNA levels increased again during adulthood (60 days). The pattern of IGFBP-5 mRNA was inversely expressed, with maximum values occurring at 40 days. IGF-I mRNA levels were higher in males at 10 days, but higher in females at 20 days. The expression of IGF-II was higher in males both at 10 and 20 days. IGFBP-5 gene expression was higher in females at 40 days. Conclusion: The dramatic changes in the expression of IGF-I, IGF-II and IGFBP-5 throughout postnatal development suggest that these factors play important roles in the development of this gland and that their actions are highly interrelated.

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“…Earlier it was thought that estrogen prevented osteoporosis exclusively by inhibiting bone resorption. Later studies revealed that estrogen stimulated cell proliferation in a dose dependent manner and increased the steady state levels of mRNA encoding a1 chain of type I collagen [Ernst et al, 1988;Nasu et al, 2000]. In vivo administration of estradiol to rats also could enhance the proaI collagen gene expression in the uterus [Komm et al, 1987].…”

Section: Estrogen As a Regulator Of Collagen Synthesismentioning

confidence: 99%

Factors influencing collagen biosynthesis

Kavitha¹,

Thampan²

2008

J of Cellular Biochemistry

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The importance of collagen, the major structural protein of animal kingdom, in maintaining the normal structure and function of the skin is well known. The same property is exploited widely in medical and industrial fields in finding agents, which could influence the synthesis of this protein. In this context in vitro production of collagen is of high significance. A literature survey has been made to analyze the various factors that influence collagen biosynthesis. There are various physical and biological factors that can either induce or inhibit collagen biosynthesis at various levels of gene expression. However reports concentrating on the effects of plants-derived compounds in stimulating collagen synthesis are scanty. Since extracts of many plants are known to be beneficial in the wound healing process, plants-derived compounds will have a definite role in the regulation of collagen synthesis. The present study emphasizes the need for unearthing the role of these plant derived factors on collagen synthesis which will be of immense application in the medical field.

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Estrogen modulates osteoblast proliferation and function regulated by parathyroid hormone in osteoblastic SaOS-2 cells: role of insulin-like growth factor (IGF)-I and IGF-binding protein-5

Cited by 37 publications

References 43 publications

Gender differences in the response of CD-1 mouse bone to parathyroid hormone: potential role of IGF-I

Gender differences in the response of CD-1 mouse bone to parathyroid hormone: potential role of IGF-I

Inverse Correlation between Insulin-Like Growth Factor (IGF)-Binding Protein-5 and IGF-I and II during Postnatal Development of the Anterior Pituitary Gland

Factors influencing collagen biosynthesis

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