Estrogen modulates vascular smooth muscle cell function through downregulation of SIRT1

Lee, Chien-Hsing; Su, Sheng-Chiang; Chiang, Chi-Fu; Chien, Chu-Yen; Hsu, Chia-Chen; Yu, T.-M.; Huang, Shih‐Ming; Shieh, Yi‐Shing; Kao, Hong-Wei; Tsai, Chien‐Sung; Hung, Yi‐Jen; Lin, Chih-Yuan

doi:10.18632/oncotarget.22546

Cited by 21 publications

(17 citation statements)

References 51 publications

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“…SIRTs are a family of NAD + dependent histone/protein deacetylases highly conserved through bacteria and eukaryotes [ 111 ]. SIRT1 has been demonstrated to inhibit oxidative stress and inflammation [ 112 , 113 ] and deacetylates a wide range of substrates, including p53, NF-κB, FOXO transcription factors, Ku-70 and PGC-1 α, with roles in cellular processes ranging from energy metabolism to cell survival. Many studies in recent years have highlighted the correlation of SIRT1 activity with AMPK and mTOR pathway [ 114 – 116 ].…”

Section: Introductionmentioning

confidence: 99%

Oxidative stress: role of physical exercise and antioxidant nutraceuticals in adulthood and aging

et al. 2018

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Physical exercise is considered to be one of the beneficial factors of a proper lifestyle and is nowadays seen as an indispensable element for good health, able to lower the risk of disorders of the cardiovascular, endocrine and osteomuscular apparatus, immune system diseases and the onset of potential neoplasms. A moderate and programmed physical exercise has often been reported to be therapeutic both in the adulthood and in aging, since capable to promote fitness. Regular exercise alleviates the negative effects caused by free radicals and offers many health benefits, including reduced risk of all-cause mortality, sarcopenia in the skeletal muscle, chronic disease, and premature death in elderly people. However, physical performance is also known to induce oxidative stress, inflammation, and muscle fatigue. Many efforts have been carried out to identify micronutrients and natural compounds, also known as nutraceuticals, able to prevent or attenuate the exercise-induced oxidative stress and inflammation.The aim of this review is to discuss the benefits deriving from a constant physical activity and by the intake of antioxidant compounds to protect the body from oxidative stress. The attention will be focused mainly on three natural antioxidants, which are quercetin, resveratrol and curcumin. Their properties and activity will be described, as well as their benefits on physical activity and on aging, which is expected to increase through the years and can get favorable benefits from a constant exercise activity.

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Section: Introductionmentioning

confidence: 99%

Oxidative stress: role of physical exercise and antioxidant nutraceuticals in adulthood and aging

et al. 2018

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“…Although production of estrogen declined dramatically in females after menopause status, the estrogen remained at moderately low level. SIRT1, possibly through the AKT and ERK signaling pathways, plays a crucial role in estrogen in protecting arteries from senescence and atherosclerosis [47]. Resveratrol also functions as a suppressor of PM-induced in ammatory signaling pathways by inhibiting COX-2/PGE2 expression [48].…”

Section: Discussionmentioning

confidence: 99%

Interaction of Sirtuin 1 (SIRT1) Candidate Longevity Gene and Particulate Matter (PM2.5) on All-cause Mortality: a Longitudinal Cohort Study in China

Yao

Liu

Guo

et al. 2020

Preprint

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BackgroundThe SIRT1 gene was associated with the lifespan in several organisms through inflammatory and oxidative stress pathways. Long-term air particulate matter (PM) is detrimental to health through the same pathways. MethodsWe used the Chinese Longitudinal Healthy Longevity Survey (CLHLS) to investigate whether there is a gene-environment (G×E) interaction of SIRT1 and air pollution on mortality in an older cohort in China. Among 7,083 participants with a mean age of 81.1 years, we genotyped the SIRT1 alleles for each participant and assessed PM2.5 concentration using 3-year average concentrations around each participant's residence. We used Cox-proportional hazards models to estimate the independent and joint effects of SIRT1 polymorphisms and PM2.5 exposure on all-cause mortality, adjusting for a set of confounders. ResultsThere were 2,843 deaths over 42,852 person-years. The mortality hazard ratio (HR) and 95% confidence interval (CI) for each 10 μg/m³ increase in PM2·5 was 1.08 (1.05-1.11); for SIRT1_391 was 0.77 (0.61, 0.98) in the recessive model after adjustment. In stratified analyses, participants carrying two SIRT1_391 minor alleles had a significantly higher HR for each 10 μg/m³ increase in PM2.5 than those carrying one or none minor allele (1.336 [1.079-1.653] vs. 1.078 [1.049-1.107]; p for interaction = 0.012). Moreover, the interaction of SIRT1 and air pollution on mortality is significant among women but not among men. We did not see significant relationships for SIRT1_366, SIRT1_773, and SIRT1_720. ConclusionWe found a gene-environment interaction of SIRT1 and air pollution on mortality, possibly through the inflammation modulating mechanism of SIRT1 polymorphisms.

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“…This non-genomic regulation may explain our current results that CD38 gene deletion markedly increases SIRT1 protein levels without significant effect on its mRNA expression. Several studies demonstrated the down-regulation of SIRT1 protein levels by E2 treatment in vascular smooth muscle cells (16, 33), but there was no data showing that E2 had effects on SIRT1 gene expression. In the present study, E2 induced obvious decrease in SIRT1 mRNA and protein levels in WT ASMCs but not in CD38 KO cells, suggesting that other unknown mechanisms may exist in E2's actions associated with CD38 and warrants further investigation in our feature work.…”

Section: Discussionmentioning

confidence: 99%

“…Estrogens were shown to increase CD38 gene expression and leads to increased calcium mobilization and contractility of the myometrium (14, 15). It has also been recently reported that E2 downregulated SIRT1 expression in vascular smooth muscle cells, with increased apoptosis, reduced proliferation and migration, which were reversed by the SIRT1 activator Resveratrol (16). SIRT1 regulates p53-dependent apoptosis by deacetylating the Lys382 residue of p53, thus enhancing the transcriptional activity of p53 and inhibiting p53-induced apoptosis (17).…”

Section: Introductionmentioning

confidence: 91%

17β-Estradiol Promotes Apoptosis in Airway Smooth Muscle Cells Through CD38/SIRT1/p53 Pathway

Liu

Guo²,

Huang

et al. 2018

Front. Endocrinol.

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17β-Estradiol (E2) is the major estrogen secreted by the premenopausal ovary and shows dual effects on cell apoptosis under pathological conditions. E2 was previously shown to increase CD38 mRNA and protein expression in myometrial smooth muscle, but its function and mechanism remain largely unknown. Here we investigated the role of E2 in hypoxia-induced apoptosis in mouse airway smooth muscle cells (ASMCs) and explored the underlying mechanisms. Results showed that E2 significantly increased CD38 expression at both mRNA and protein levels, accompanied with decreased SIRT1 levels in ASMCs. By using primary ASMCs from the wild type (WT) and the smooth muscle-specific CD38 knockout (CD38 KO) mice, we found that the down-regulation of SIRT1 induced by E2 was abolished in CD38 KO AMSCs. E2 promoted the acetylation of p53 in WT cells, and this effect was also diminished in the absence of CD38. In addition, E2 further activated CD38/SIRT1/p53 signal pathway and promoted cell apoptosis during hypoxia. However, these effects were reversed in CD38 KO ASMCs and by the specific SIRT1 activator Resveratrol. We also found that E2 enhanced CD38 expression through estrogen receptor. The data suggested that CD38 is a direct target for E2 which promotes hypoxia-induced AMSC apoptosis through SIRT1/p53 signal pathway.

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Estrogen modulates vascular smooth muscle cell function through downregulation of SIRT1

Cited by 21 publications

References 51 publications

Oxidative stress: role of physical exercise and antioxidant nutraceuticals in adulthood and aging

Oxidative stress: role of physical exercise and antioxidant nutraceuticals in adulthood and aging

Interaction of Sirtuin 1 (SIRT1) Candidate Longevity Gene and Particulate Matter (PM2.5) on All-cause Mortality: a Longitudinal Cohort Study in China

17β-Estradiol Promotes Apoptosis in Airway Smooth Muscle Cells Through CD38/SIRT1/p53 Pathway

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