Estrogen: Physiology, Pharmacology, and Formulations for Replacement Therapy

Ruggiero, Ronald J.; Likis, Frances E

doi:10.1016/s1526-9523(02)00233-7

Cited by 88 publications

(59 citation statements)

References 16 publications

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“…Regarding hormone replacement therapy, in recent years, concerns have been raised by studies such as the Women's Health Initiative, regarding the risks associated with the use of synthetically produced hormones [4]. Combining the autologous nature of iPSCs with the potential to differentiate iPSC-derived EBs into steroidogenic cells, it will be possible to produce bioidentical hormones for patient treatment [38]. A potential future direction with the presented microfluidic platform will be to further evaluate human iPSCs for clinically relevant applications.…”

Section: Toward Personalized Medicinementioning

confidence: 99%

Functional Maintenance of Differentiated Embryoid Bodies in Microfluidic Systems: A Platform for Personalized Medicine

Güven

Lindsey

Poudel

et al. 2015

Stem Cells Translational Medicine

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Hormone replacement therapies have become important for treating diseases such as premature ovarian failure or menopausal complications. The clinical use of bioidentical hormones might significantly reduce some of the potential risks reportedly associated with the use of synthetic hormones. In the present study, we demonstrate the utility and advantage of a microfluidic chip culture system to enhance the development of personalized, on-demand, treatment modules using embryoid bodies (EBs). Functional EBs cultured on microfluidic chips represent a platform for personalized, patient-specific treatment cassettes that can be cryopreserved until required for treatment. We assessed the viability, differentiation, and functionality of EBs cultured and cryopreserved in this system. During extended microfluidic culture, estradiol, progesterone, testosterone, and anti-müllerian hormone levels were measured, and the expression of differentiated steroidogenic cells was confirmed by immunocytochemistry assay for the ovarian tissue markers anti-müllerian hormone receptor type II, follicle-stimulating hormone receptor, and inhibin b-A and the estrogen biosynthesis enzyme aromatase. Our studies showed that under microfluidic conditions, differentiated steroidogenic EBs continued to secrete estradiol and progesterone at physiologically relevant concentrations (30-120 pg/ml and 150-450 pg/ml, respectively) for up to 21 days. Collectively, we have demonstrated for the first time the feasibility of using a microfluidic chip system with continuous flow for the differentiation and extended culture of functional steroidogenic stem cell-derived EBs, the differentiation of EBs into cells expressing ovarian antigens in a microfluidic system, and the ability to cryopreserve this system with restoration of growth and functionality on thawing. These results present a platform for the development of a new therapeutic system for personalized medicine. STEM CELLS TRANSLATIONAL MEDICINE 2015;4:261-268

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Section: Toward Personalized Medicinementioning

confidence: 99%

Functional Maintenance of Differentiated Embryoid Bodies in Microfluidic Systems: A Platform for Personalized Medicine

Güven

Lindsey

Poudel

et al. 2015

Stem Cells Translational Medicine

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“…Abbott IMX [19] Corning ACS:180 [8] DPC Coat-a-Count [16] In-House Extraction [4] DPC Double Ab [22] Boehringer ES700 [5] DPC E2-6 [8] Boehringer ES300 [8] IDS Gamma-B [4] Wallac DELFIA [22] J&J Amerlite [9] DPC Immulite [9] Sorin [4] Serono Maia [4] INCStar Clin Assays [7] remaining in-house extraction RIA users have often performed better than market-leading immunoassay platforms in terms of comparability (see Figure 2) and recovery. Only three laboratories in the UK maintain these assays.…”

Section: Oestradiol Bias and Variability Of The Bias (Var) Versus Id-mentioning

confidence: 99%

“…These include effects on the bone, skin and muscle, liver, brain, vascular function and lipid metabolism. Good reviews of the various functions of oestradiol have been written by Ruggerio et al 4 and Gruber et al…”

Section: Introductionmentioning

confidence: 99%

Oestradiol assays: fitness for purpose?

Middle

Kane

2009

Ann Clin Biochem

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In this review we discuss the analytical inadequacies of oestradiol assays in relation to the clinical requirements for performing them, and make recommendations for their improvement. The measurement of oestradiol can be requested in a number of clinical scenarios ( precocious puberty, infertility, assisted conception, hormone replacement therapy). The very wide dynamic range of oestradiol concentrations is a huge challenge for routine assays, which they are unlikely to meet on theoretical as well as practical grounds. The EQA performance of oestradiol assays in terms of trueness, comparability, recovery and analytical sensitivity leaves much to be desired and indicates that calibration is compromised by poor analytical specificity. To make oestradiol assays fit for purpose requires concerted action by all stakeholders to define analytical quality specifications for the various clinical scenarios involved, and then to encourage concerted action by the diagnostic industry to use the steroid reference measurement system to improve specificity, trueness and traceability.

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“…However, estrogen is a pleiotropic hormone that has multiple physiological functions (15) and its effects in the body are dependent on its route of administration (16). When taken orally, most 17β-estradiol (E2) is converted in the liver into estrone (E1), which is 10-fold less potent than E2 (17). In addition, the resulting supraphysiological level of estrogen in the…”

mentioning

confidence: 99%

Reducing posttreatment relapse in cleft lip palatal expansion using an injectable estrogen–nanodiamond hydrogel

Hong

Song

Lee

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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Patients with cleft lip and/or palate (CLP), who undergo numerous medical interventions from infancy, can suffer from lifelong debilitation caused by underdeveloped maxillae. Conventional treatment approaches use maxillary expansion techniques to develop normal speech, achieve functional occlusion for nutrition intake, and improve esthetics. However, as patients with CLP congenitally lack bone in the cleft site with diminished capacity for bone formation in the expanded palate, more than 80% of the patient population experiences significant postexpansion relapse. While such relapse has been a long-standing battle in craniofacial care of patients, currently there are no available strategies to address this pervasive problem. Estrogen, 17β-estradiol (E2), is a powerful therapeutic agent that plays a critical role in bone homeostasis. However, E2's clinical application is less appreciated due to several limitations, including its pleiotropic effects and short half-life. Here, we developed a treatment strategy using an injectable system with photo-crosslinkable hydrogel (G) and nanodiamond (ND) technology to facilitate the targeted and sustained delivery of E2 to promote bone formation. In a preclinical expansion/relapse model, this functionalized E2/ ND/G complex substantially reduced postexpansion relapse by nearly threefold through enhancements in sutural remodeling compared with unmodified E2 administration. The E2/ND/G group demonstrated greater bone volume by twofold and higher osteoblast number by threefold, compared with the control group. The E2/ND/G platform maximized the beneficial effects of E2 through its extended release with superior efficacy and safety at the local level. This broadly applicable E2 delivery platform shows promise as an adjuvant therapy in craniofacial care of patients.nanomedicine | cleft palate | craniofacial medicine | nanodiamond | drug delivery

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Estrogen: Physiology, Pharmacology, and Formulations for Replacement Therapy

Cited by 88 publications

References 16 publications

Functional Maintenance of Differentiated Embryoid Bodies in Microfluidic Systems: A Platform for Personalized Medicine

Functional Maintenance of Differentiated Embryoid Bodies in Microfluidic Systems: A Platform for Personalized Medicine

Oestradiol assays: fitness for purpose?

Reducing posttreatment relapse in cleft lip palatal expansion using an injectable estrogen–nanodiamond hydrogel

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