Estrogen receptor 2b deficiency impairs the antiviral response of zebrafish

López-Muñoz, Azucena; Liarte, Sergio; Gómez-González, Núria E.; Cabas, Isabel; Meseguer, José; García‐Ayala, Alfonsa; Mulero, Víctoriano

doi:10.1016/j.dci.2015.06.008

Cited by 18 publications

(19 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Two typical IFN-inducible genes, pkr and pkz, can suppress replication of grass carp reovirus (51,52). To date, zebrafish have been widely employed to investigate the function of genes involved in innate immunity (43,(53)(54)(55). In this study, we showed that infection with high titers of SVCV can result in body degeneration of zebrafish larvae and eventual death.…”

Section: Discussionmentioning

confidence: 73%

“…As shown in Fig. 1A, foxo3b mRNA was increased after treatment with poly(I:C) for 12 h and peaked at 24 h. Subsequently, zebrafish larvae (3 d postfertilization [dpf]) The 43,44), foxo3b mRNA was greatly induced (Fig. 1B).…”

Section: Zebrafish Foxo3b Is Induced Upon Poly(i:c) or Svcv Stimulationmentioning

confidence: 81%

“…To determine the role of foxo3b in response to viral infection, we examined the expressions of ifn1, pkz, mxc, and lta (four welldefined irf3/irf7 downstream genes of zebrafish) in response to viral infection (43,44). As shown in Fig.…”

Section: Knockout Of Foxo3b In Zebrafish Enhances Antiviral Responsementioning

confidence: 99%

See 2 more Smart Citations

Zebrafish foxo3b Negatively Regulates Antiviral Response through Suppressing the Transactivity of irf3 and irf7

Xing

Cai

Zhang

et al. 2016

The Journal of Immunology

View full text Add to dashboard Cite

Forkhead box O (FOXO)3, a member of the FOXO family of transcription factors, plays key roles in various cellular processes, including development, longevity, reproduction, and metabolism. Recently, FOXO3 has also been shown to be involved in modulating the immune response. However, how FOXO3 regulates immunity and the underlying mechanisms are still largely unknown. In this study, we show that zebrafish (Danio rerio) foxo3b, an ortholog of mammalian FOXO3, is induced by polyinosinic-polycytidylic acid stimulation and spring viremia of carp virus (SVCV) infection. We found that foxo3b interacted with irf3 and irf7 to inhibit ifr3/irf7 transcriptional activity, thus resulting in suppression of SVCV or polyinosinic-polycytidylic acid–induced IFN activation. By suppressing expression of key antiviral genes, foxo3b negatively regulated the cellular antiviral response. Furthermore, upon SVCV infection, the expression of the key antiviral genes was significantly enhanced in foxo3b-null zebrafish larvae compared with wild-type larvae. Additionally, the replication of SVCV was inhibited in foxo3b-null zebrafish larvae, leading to a higher survival rate. Our findings suggest that by suppressing irf3/irf7 activity, zebrafish foxo3b negatively regulates the antiviral response, implicating the vital role of the FOXO gene family in innate immunity.

show abstract

Section: Discussionmentioning

confidence: 73%

Section: Zebrafish Foxo3b Is Induced Upon Poly(i:c) or Svcv Stimulationmentioning

confidence: 81%

See 1 more Smart Citation

Zebrafish foxo3b Negatively Regulates Antiviral Response through Suppressing the Transactivity of irf3 and irf7

Xing

Cai

Zhang

et al. 2016

The Journal of Immunology

View full text Add to dashboard Cite

show abstract

“…Induction of specific target genes of ERα and ERβ2 could also be inhibited by morpholino knockdown of the receptor (Griffin et al 2013). Recently, a mutant zebrafish line has been identified, carrying a mutation in the gene encoding ERβ2 (resulting in an 8 amino acid insertion in the ligand-binding domain (Lopez-Munoz et al 2015). This mutation resulted in distorted sexual ratios (characterized by an increased adult male population), altered testicular morphology and increased testosterone and 17β-estradiol (E2) levels.…”

Section: The Zebrafish Estrogen Receptors (Ers)mentioning

confidence: 99%

“…This mutation resulted in distorted sexual ratios (characterized by an increased adult male population), altered testicular morphology and increased testosterone and 17β-estradiol (E2) levels. In addition, these fish show a decreased immune response to a viral infection (Lopez-Munoz et al 2015). Further analysis of the literature revealed that about half of the published articles on the zebrafish ER involves research on the role of this receptor as a mediator of the activity of endocrine-disrupting chemicals (EDCs).…”

Section: The Zebrafish Estrogen Receptors (Ers)mentioning

confidence: 99%

Nuclear receptor research in zebrafish

Schaaf

2017

Journal of Molecular Endocrinology

View full text Add to dashboard Cite

Nuclear receptors (NRs) form a superfamily of transcription factors that can be activated by ligands and are involved in a wide range of physiological processes. NRs are well conserved between vertebrate species. The zebrafish, an increasingly popular animal model system, contains a total of 73 NR genes, and orthologues of almost all human NRs are present. In this review article, an overview is presented of NR research in which the zebrafish has been used as a model. Research is described on the three most studied zebrafish NRs: the estrogen receptors (ERs), retinoic acid receptors (RARs) and peroxisome proliferator-activated receptors (PPARs). The studies on these receptors illustrate the versatility of the zebrafish as a model for ecotoxicological, developmental and biomedical research. Although the use of the zebrafish in NR research is still relatively limited, it is expected that in the next decade the full potential of this animal model will be exploited.

show abstract