Estrogen Receptor and Progesterone Receptor Status in Breast Cancer in Relation to Age, Histological Grade, Size of Lesion and Lymph Node Involvement

Sofi, Gulam Nabi; Sofi, Junaid Nabi; Nadeem, Raja; Shiekh, Rayees Yousuf; Khan, Faroze Ahmad; Sofi, Abid Ahmad; Bhat, Hillal Ahmad; Bhat, Rayees Ahmad

doi:10.7314/apjcp.2012.13.10.5047

Cited by 63 publications

(71 citation statements)

References 47 publications

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“…It was noticed that the most common histological type of breast cancer was invasive ductal carcinoma in our country. This result is supported by previous studies who reported that most of the breast cancer cases undergoing surgical resection are GII and GIII of IDC [20,21].…”

Section: Discussionsupporting

confidence: 82%

Immunohistochemical Expression of Cyclin D1 in Invasive Ductal Carcinoma of Human Breast

Assem¹,

Youssef²,

Rashad³

et al. 2017

Oncomedicine

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Cyclin D1 has been observed in many human tumors and is likely to promote cell proliferation and differentiation by shortening the G1/S transition. This work was aimed to evaluate the prognostic role of Cyclin D1 in invasive ductal carcinoma (IDC) and its correlation with other established prognostic parameters of breast cancer. This study was conducted on 40 prospective biopsies taken from patients with breast tumor; 30 cases having IDC and 10 cases with benign tumor as well as 10 normal biopsies. Sections from all cases were subjected to stain with haematoxylin and eosin (H&E) for histopathological examination and immunohistochemical technique for Cyclin D1 in addition using flow cytometric technique to perform cell cycle analysis. Histopathological results showed fibroadenoma and fibrocystic disease in benign cases and IDC with 60% grade II and 40% grade III in malignant cases. Immunohistochemical results revealed higher expression of Cyclin D1 in IDC comparing to normal and benign breast tissues. Statistically there was significant correlation between Cyclin D1 scores and the positivity of estrogen receptor (ER) and progesterone receptor (PR), and no significant correlation was seen between Cyclin D1 and S-phase %. It could be suggested that Cyclin D1 protein may be a valuable prognostic marker in IDC and can be targeted in the future therapeutic approaches.

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Section: Discussionsupporting

confidence: 82%

Immunohistochemical Expression of Cyclin D1 in Invasive Ductal Carcinoma of Human Breast

Assem¹,

Youssef²,

Rashad³

et al. 2017

Oncomedicine

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“…Paraffin embedded tissue sections from invasive ductal carcinoma of breast were used for immunohistochemical analysis of Estrogen and Progesterone receptors using specific antibodies against corresponding proteins as described in Materials and methods, sections were counter stained with hematoxylin study, 69.5% of tumors were found to be ER-positive and 66.4% of tumors were found to be PR positive ( Figure 4). Our results of hormone receptor positivity are almost similar to an earlier investigation conducted on the breast cancer patients from Kashmir (Sofi et al, 2012). However, we did not find any correlation between methylation of CDH1 and estrogen or progesterone receptor status although previous studies have reported such associations (Shinozaki et al, 2005;Caldeira et al, 2006;Li et al, 2006;Ronneberg et al, 2011).…”

Section: Discussionsupporting

confidence: 74%

Loss of Expression and Aberrant Methylation of the CDH1 (E-cadherin) Gene in Breast Cancer Patients from Kashmir

Asiaf

Ahmad²,

Aziz³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Background: Aberrant promoter hypermethylation has been recognized in human breast carcinogenesis as a frequent molecular alteration associated with the loss of expression of a number of key regulatory genes and may serve as a biomarker. The E-cadherin gene (CDH1), mapping at chromosome 16q22, is an intercellular adhesion molecule in epithelial cells, which plays an important role in establishing and maintaining intercellular connections. The aim of our study was to assess the methylation pattern of CDH1 and to correlate it with the expression of E-cadherin, clinicopathological parameters and hormone receptor status in breast cancer patients of Kashmir. Materials and Methods: Methylation specific PCR (MSP) was used to determine the methylation status of CDH1 in 128 invasive ductal carcinomas (IDCs) paired with the corresponding normal tissue samples. Immunohistochemistry was used to study the expression of E-cadherin, ER and PR. Results: CDH1 hypermethylation was detected in 57.8% of cases and 14.8% of normal adjacent controls. Reduced levels of E-cadherin protein were observed in 71.9% of our samples. Loss of E-cadherin expression was significantly associated with the CDH1 promoter region methylation (p<0.05, OR=3.48, CI: 1.55-7.79). Hypermethylation of CDH1 was significantly associated with age at diagnosis (p=0.030), tumor size (p=0.008), tumor grade (p= 0.024) and rate of node positivity or metastasis (p=0.043). Conclusions: Our preliminary findings suggest that abnormal CDH1 methylation occurs in high frequencies in infiltrating breast cancers associated with a decrease in E-cadherin expression. We found significant differences in tumor-related CDH1 gene methylation patterns relevant to tumor grade, tumor size, nodal involvement and age at diagnosis of breast tumors, which could be extended in future to provide diagnostic and prognostic information.

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“…However, young patients less than or equal to 40 years of age have more unfavourable hormone receptor status (61.11%) in comparison to patients above 40 years of age(unfavourable hormone receptor status= 48%). This observation is in concordance with other studies which state that breast cancers in young women have unfavourable biological behaviour and poor prognosis, than breast cancers in older women [8][9][10].…”

Section: Discussionsupporting

confidence: 82%

“…As these tumors have good prognosis, these tumors are considered to have favourable hormone receptor expression [6][7][8].…”

Section: Methodsmentioning

confidence: 99%

Predictive and Prognostic Factors in Breast Cancer and their Association with ER PR HER2/neu Expression

Sunita¹,

Nath²

2016

J Carcinog Mutagen

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Estrogen Receptor and Progesterone Receptor Status in Breast Cancer in Relation to Age, Histological Grade, Size of Lesion and Lymph Node Involvement

Cited by 63 publications

References 47 publications

Immunohistochemical Expression of Cyclin D1 in Invasive Ductal Carcinoma of Human Breast

Immunohistochemical Expression of Cyclin D1 in Invasive Ductal Carcinoma of Human Breast

Loss of Expression and Aberrant Methylation of the CDH1 (E-cadherin) Gene in Breast Cancer Patients from Kashmir

Predictive and Prognostic Factors in Breast Cancer and their Association with ER PR HER2/neu Expression

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