Estrogen receptors in medaka (Oryzias latipes) and estrogenic environmental contaminants: An in vitro–in vivo correlation

Chakraborty, Tapas; Katsu, Yoshinao; Zhou, Lin; Miyagawa, Shinichi; Nagahama, Yoshitaka; Iguchi, Taisen

doi:10.1016/j.jsbmb.2010.11.015

Cited by 45 publications

(41 citation statements)

References 35 publications

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“…6B). Although we are unaware of any studies that have assessed expression of vtgr in isolated follicles after hormone treatment, a recent study found that aqueous exposure of medaka to E 2 caused a decrease in female vtgr mRNA levels in concert with increased esr2b mRNA expression in gonad tissues [29]. We failed to see modulation of expression for any esr subtypes by E 2 nor did we see changes in ar expression following the 11-KT treatment.…”

contrasting

confidence: 56%

“…Such investigations have been limited to insects [28], which have disparate hormonal pathways compared to vertebrates (i.e., insects use ecdysone, which is not present in fish and other vertebrates) possibly limiting the relevance of fish hormonal contributions to Vtgr regulation. Only one recent study using the medaka model revealed that E 2 exposure suppresses vtgr expression in females in concert with potential infertility [29]. Using the largemouth bass (LMB; Micropterus salmoides) model, which have a semisynchronized reproductive cycle that allows monitoring of endocrine biology in several discrete windows of the reproductive process, our goal was to identify and classify the LMB vtgr cDNA using various PCR strategies and phylogenetic analysis.…”

Section: Introductionmentioning

confidence: 99%

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Identification and Transcriptional Modulation of the Largemouth Bass, Micropterus salmoides, Vitellogenin Receptor During Oocyte Development by Insulin and Sex Steroids1

Domínguez¹,

Quattro²,

Denslow³

et al. 2012

Biology of Reproduction

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Fish vitellogenin synthesized and released from the liver of oviparous animals is taken up into oocytes by the vitellogenin receptor. This is an essential process in providing nutrient yolk to developing embryos to ensure successful reproduction. Here we disclose the full length vtgr cDNA sequence for largemouth bass (LMB) that reveals greater than 90% sequence homology with other fish vtgr sequences. We classify LMB Vtgr as a member of the low density lipoprotein receptor superfamily based on conserved domains and categorize as the short variant that is devoid of the O-glycan segment. Phylogenetic analysis places LMB Vtgr sequence into a well-supported monophyletic group of fish Vtgr. Real-time PCR showed that the greatest levels of LMB vtgr mRNA expression occurred in previtellogenic ovarian tissues. In addition, we reveal the effects of insulin, 17beta-estradiol (E 2 ), and 11-ketotestosterone (11-KT) in modulation of vtgr, esr, and ar mRNAs in previtellogenic oocytes. Insulin increased vtgr expression levels in follicles ex vivo while exposure to E 2 or 11-KT did not result in modulation of expression. However, both steroids were able to repress insulin-induced vtgr transcript levels. Coexposure with insulin and E 2 or of insulin and 11-KT increased ovarian esr2b and ar mRNA levels, respectively, which suggest a role for these nuclear receptors in insulin-mediated signaling pathways. These data provide the first evidence for the ordered stage-specific expression of LMB vtgr during the normal reproductive process and the hormonal influence of insulin and sex steroids on controlling vtgr transcript levels in ovarian tissues.insulin, oocyte development, steroid hormones, vitellogenin, vitellogenin receptor

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contrasting

confidence: 56%

Section: Introductionmentioning

confidence: 99%

Identification and Transcriptional Modulation of the Largemouth Bass, Micropterus salmoides, Vitellogenin Receptor During Oocyte Development by Insulin and Sex Steroids1

Domínguez¹,

Quattro²,

Denslow³

et al. 2012

Biology of Reproduction

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“…Medaka fish express least three distinct types of ER (ERα, ERβ1 and ERβ2) (Hawkins et al, 2000;Chakraborty et al, 2011). The complete estrogen receptor family identified in pseudotetraploid rainbow trout consists of two subtypes (ERα and ERβ) each of which consists of two isoforms (α1/α2 and β1/β2), and the levels of ERα1 mRNA are more easily affected by estradiol (E2) and EE2 than those of other isoforms (Boyce-Derricott et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Long-term exposure investigating the estrogenic potency of estriol in Japanese medaka (Oryzias latipes)

Lei

Kang

et al. 2014

Comparative Biochemistry and Physiology Part C: Toxicology &

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The growth, development, and ERα and Vtg-I gene expressions of Japanese ricefish (Oryzias latipes; medaka) exposed to different concentrations of estriol (E3), including one environmentally relevant concentration, during embryo-adult life stages were evaluated. At the early life stage, fertilized eggs were exposed to 5, 50, 500, 5000 ng/L E3 for 15 days, and the hatched fry were exposed continuously to the same concentrations for an additional 15 days. Exposure to 500 and 5000 ng/L E3 resulted in adverse effects on hatchability and time to hatching. At 5000 ng/L, the gross abnormality rate was increased and the number of females that hatched was twice that of males. When the fish were exposed to 5-5000 ng/L E3 for further 60 days, the male hepatosomatic index (HSI) was increased at 5000 ng/L. The female gonadosomatic index (GSI) was decreased at 500 and 5000 ng/L E3, while the male GSI at 5000 ng/L E3 was increased and sex reversal was also found at this concentration. Quantitative RT-PCR showed that the hepatic vitellogenin-I (Vtg-I) genes were up-regulated in females at 500 and 5000 ng/L E3 and in males at all E3 concentrations, whereas E3 did not affect estrogen receptor α (ERα) mRNA transcription. These results showed that E3 at environmental concentration of 5 ng/L has no adverse effects on growth and development of the Japanese medaka. However, in this study, if we only focused on Vtg gene change in males, E3 had strong estrogenic effects on male medaka under the conditions of these experiments.

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“…This result was incongruent with fin phenotypes, because it is thought that oestrogen might drive feminine hormones controlling the secondary characteristics of females. A well known fact in teleosts fishes is that oestrogens not only play crucial roles in reproductive functions of females but also are important in the reproductive functions of males, including the development of secondary sex characteristics and behaviour (Muramatsu and Inoue 2000, Chakraborty et al 2011, Cheung et al 2013. This result suggests that the pectoral fins of males may be the target for oestrogens, even though oestrogens affect that coordinate functions via ERα in these fins have so far been discussed as being related to sex characteristics.…”

Section: Discussionmentioning

confidence: 99%

“…Among the various teleosts species, the fishes of the genus Oryzias are notable for their extensive use as vertebrate model organisms for research in many fields, such as endocrinology, developmental biology, and reproductive biology (Chakraborty et al 2011, Ismail and Yusof 2011, Zhang et al 2013. In this genus, the dorsal and anal fins are suggested to be typical secondary sex characteristics.…”

Section: Introductionmentioning

confidence: 99%

Investigation of hormone receptor expressions in the fins of Oryzias woworae (Actinopterygii: Beloniformes: Adrianichthyidae)

Ngamniyom¹,

Sriyapai²,

Somyoonsap³

2014

Acta Ichthyol. Piscat

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Ngamniyom A., Sriyapai T., Somyoonsap P. 2014. Investigation of hormone receptor expressions in the fins of Oryzias woworae (Actinopterygii: Beloniformes: Adrianichthyidae). Acta Ichthyol. Piscat. 44 (3): 221-227.Background. In the genus Oryzias, the morphology of the dorsal and anal fin constitute typical secondary sex characteristics that are controlled by sex steroid hormones through hormone receptors. However, the level expressions of hormone receptors in fish fins of this genus have remained to be clarified. To achieve the aims of this study, we completely examined the patterns of hormone receptor expression in all fin types of adult Oryzias woworae Parenti et Hadiaty, 2010. Materials and methods. The androgen receptor (AR) α, ARβ, oestrogen receptor (ER) α, and ERβ expression in the dorsal-, anal-, pectoral-, pelvic-, and caudal fins, including the dorsal and ventral edges of the caudal fins, were determined using semi-quantitative RT-PCR. Results. Hormone receptor expression levels were significantly different in the dorsal-, anal-, and caudal fins of males, including the dorsal and ventral edges of their caudal fins, and in the pectoral and pelvic fins, and the dorsal and ventral edges of the caudal fins of females. ARα and ARβ levels in dorsal fins and ARβ levels in anal and caudal fins were higher in males than in females. ERα levels in pectoral fins were higher in males than in females. Conversely, ERβ levels in the pectoral and pelvic fins and in the dorsal and ventral edges of caudal fins were higher in females than in males. Conclusion. These results suggest that AR-and ER-mediated functions may regulate sexual dimorphism, and that characteristics of fin morphology are dependent on androgen and oestrogen regulation in adult Oryzias woworae.

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Estrogen receptors in medaka (Oryzias latipes) and estrogenic environmental contaminants: An in vitro–in vivo correlation

Cited by 45 publications

References 35 publications

Identification and Transcriptional Modulation of the Largemouth Bass, Micropterus salmoides, Vitellogenin Receptor During Oocyte Development by Insulin and Sex Steroids1

Identification and Transcriptional Modulation of the Largemouth Bass, Micropterus salmoides, Vitellogenin Receptor During Oocyte Development by Insulin and Sex Steroids1

Long-term exposure investigating the estrogenic potency of estriol in Japanese medaka (Oryzias latipes)

Investigation of hormone receptor expressions in the fins of Oryzias woworae (Actinopterygii: Beloniformes: Adrianichthyidae)

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