Estrogen Regulation of Uterine Genes In Vivo Detected by Complementary DNA Array

Abstract

We have demonstrated that cDNA array represents a powerful approach to identify key molecules in the estrogens therapy. A number of the candidates reported here should provide new markers that may contribute to the detection of target estrogen receptor. This information may also aid the development of new approaches to therapeutic intervention.

Estrogen Receptor-Dependent Genomic Responses in the Uterus Mirror the Biphasic Physiological Response to Estrogen

“…IGF-1 and IGFBP5 were both up-regulated, and IGFBP3 was repressed by estradiol treatment ( Table 3). The E regulation of these components in the mouse uterus has been previously reported (21)(22)(23). The recognition of these previously characterized E targets in our microarray data set serves as an indicator of the validity of our analysis method.…”

“…Naciff et al (54) treated rat embryos with 17␣-ethinyl estradiol or xenoestrogens on gestational d 11-20 and examined the genomic response in the developing reproductive tract as a model of embryonic exposure to estrogenic compounds. In another study, RNA was prepared from rat uterine RNA after 48 d of chronic treatment with estradiol and compared with vehicle-treated samples to identify E-regulated genes (23). In a third study, ovx rats or mice were treated with vehicle or E for 6 wk and uteri and kidneys collected for analysis to address the effect of chronic E treatment on gene expression (14).…”

Steroid Receptors in the Uterus and Ovary

“…Numerous studies have used microarray techniques to map the global gene expression patterns after estrogen exposure in the uterus and largely demonstrate that the biphasic uterine response to estrogens, so well characterized by physiological indicators discussed earlier (Table 25.4), is mirrored by the global changes in gene expression (Figure 25.13). 242,256,[315][316][317][318][319][320] The clearly defined patterns of early and late response genes found in mouse uterine tissues are completely lacking in ERα-null uteri. 159,242 The identified genes fall into functional groupings, including signal transduction, gene transcription, metabolism, protein synthesis and processing, immune function, and cell cycle.…”

What's new in estrogen receptor action in the female reproductive tract

“…Microarray analysis has significantly advanced understanding of genomic response of the rodent uterus to E2. Numerous studies have used microarray techniques to map the global gene expression patterns after estrogen exposure in the uterus and largely demonstrate that the biphasic uterine response to estrogens, so well characterized by physiological indicators above, is mirrored by the global changes in gene expression ( Andrade, et al 2002 ; Fertuck, et al 2003 ; Hewitt, et al 2005 ; Hewitt et al 2003 ; Ho Hong, et al 2004 ; Hong, et al 2006 ; Moggs, et al 2004 ; Watanabe, et al 2003 ). The clearly defined patterns of early and late response genes found in mouse uterine tissues are completely lacking in ERα–null (αERKO, Ex3αERKO) uteri ( Hewitt et al 2003 ; Hewitt, et al 2010a ).…”