2008
DOI: 10.1677/erc-07-0227
|View full text |Cite
|
Sign up to set email alerts
|

Estrogen signaling ability in human endometrial cancer through the cancer-stromal interaction

Abstract: The estrogen pathway plays an important role in the etiology of human endometrial carcinoma (EC). We examined whether estrogen biosynthesis in the tumor microenvironment promotes endometrial cancer. To examine the contribution of stromal cells to estrogen signaling in EC, we used reporter cells stably transfected with the estrogen response element (ERE) fused to the destabilized green fluorescent protein (GFP) gene. In this system, the endometrial cancer stromal cells from several patients activated the ERE of… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

4
35
0

Year Published

2009
2009
2021
2021

Publication Types

Select...
6
2

Relationship

0
8

Authors

Journals

citations
Cited by 40 publications
(39 citation statements)
references
References 38 publications
4
35
0
Order By: Relevance
“…(15) We found the similar result that while GFP levels varied among cases, stromal fibroblasts could activate ER in the presence of testosterone, which was inhibited by aromatase inhibitors. These results suggest that aromatase inhibitor might be useful for hormonal therapy in endometrial cancers.…”
Section: Estrogen Signaling In the Cancer Microenvironmentsupporting
confidence: 69%
See 2 more Smart Citations
“…(15) We found the similar result that while GFP levels varied among cases, stromal fibroblasts could activate ER in the presence of testosterone, which was inhibited by aromatase inhibitors. These results suggest that aromatase inhibitor might be useful for hormonal therapy in endometrial cancers.…”
Section: Estrogen Signaling In the Cancer Microenvironmentsupporting
confidence: 69%
“…The amplified adenovirus infected cells prepared from tissue specimens by collagenase treatment. (15) The other assay system involves a reporter cell line which stably introduced the ERE-GFP reporter gene. We established a reporter cell line, named MCF-7-E10, to visualize ER activity stimulated by extracellular signals such as estrogen or other factors.…”
Section: Imaging Of Transcription Activity Of Nuclear Receptormentioning
confidence: 99%
See 1 more Smart Citation
“…Ishikawa, RL95-2, MCF-7, and 3T3-L1 cells were cultured in RPMI 1640 (Life Technologies) supplemented with 10% fetal bovine serum (BioWest), penicillin (100 units/mL), streptomycin (100 μg/mL), and amphotericin (250 ng/mL growth medium). Primary stromal cells employed in this study were designated #3, #11, and #16 and were isolated from human endometrial carcinoma tissue samples by collagenase treatment and maintained in RPMI 1640 with 10% fetal bovine serum, penicillin (100 units/mL), streptomycin (100 μg/mL), and amphotericin as described previously (13). mRNA expressions of aromatase and 17β-HSD type 2 in stromal cells were as follows: aromatase (+) and 17β-HSD type 2 (-) in #3 and aromatase (+) and 17β-HSD type 2 (+) in both #11 and #16.…”
Section: Methodsmentioning
confidence: 99%
“…We previously examined whether estrogen biosynthesis in the tumor microenvironment promoted endometrial carcinoma or not. In order to study the contribution of stromal cells to estrogen signaling pathways in endometrial carcinoma, we examined reporter cells stably transfected with the estrogen response element (ERE) fused to the destabilized green fluorescent protein (GFP) gene (Matsumoto et al 2008). In this system, the ERE of carcinoma cells was activated to a variable extent by intratumoral stromal cells isolated from endometrial carcinoma.…”
Section: :7mentioning
confidence: 99%