Estrogen signaling in testicular cells

Carreau, Serge; Bois, Camille; Zanatta, Leïla; Silva, F.R.M.B.; Bouraïma-Lelong, Hélène; Delalande, Christelle

doi:10.1016/j.lfs.2011.06.004

Cited by 63 publications

(58 citation statements)

References 51 publications

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“…More recently, the presence of aromatase mRNA in gonocytes and preleptotene spermatocytes has also been reported. 34 In immature rats, Sertoli cells are the major source of estrogen but Leydig cells are the main source in adult animals. 35,36 Expression of aromatase is stimulated by follicle-stimulating hormone (FSH) and the maximum stimulatory effect of FSH in aromatase gene expression occurs in 20-d old rat Sertoli cells and seems to parallel Sertoli cell differentiation.…”

Section: Estrogen Synthesis and Function In The Malementioning

confidence: 99%

17β-estradiol signaling and regulation of Sertoli cell function

et al. 2011

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Section: Estrogen Synthesis and Function In The Malementioning

confidence: 99%

17β-estradiol signaling and regulation of Sertoli cell function

et al. 2011

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“…Aromatase is widely distributed in ovaries, brain, placenta, adipose tissue as well as in testes and spermatozoa of many species including humans [6,7]. The main source of estrogens in immature male rodents are Sertoli cells, whereas in adult animals, high levels of aromatase transcripts are found in Leydig cells [6].…”

Section: Introductionmentioning

confidence: 99%

“…The main source of estrogens in immature male rodents are Sertoli cells, whereas in adult animals, high levels of aromatase transcripts are found in Leydig cells [6]. It was previously reported that germ cells have also possibility to convert androgens into estrogens [6,8].…”

Section: Introductionmentioning

confidence: 99%

“…There is a general agreement that ARs can be detected within the seminiferous epithelium in nuclei of Sertoli cells, peritubular myoid cells, interstitial Leydig cells and perivascular smooth muscle cells [13]. The biological functions of estrogens are mediated by two types of estrogen receptors: a (ERa) and b (ERb) [6]. ERs regulate gene expression by direct interaction with estrogen-response elements (EREs) in the promoter of target genes [14].…”

Section: Introductionmentioning

confidence: 99%

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The immunoexpression of androgen receptor, estrogen receptors alpha and beta, vanilloid type 1 receptor and cytochrome p450 aromatase in rats testis chronically treated with letrozole, an aromatase inhibitor

Pilutin

Misiakiewicz-Has²,

Kolasa³

et al. 2014

Folia Histochem Cytobiol.

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Abstract:The function of testis is under hormonal control and any disturbance of hormonal homeostasis can lead to morphological and physiological changes. Therefore the aim of the study was to investigate the expression of androgen and estrogen receptors (AR, ERs), vanilloid receptor (TRPV1), cytochrome P450 aromatase (P450arom), as well as apoptosis of cells in testis of adult rats chronically treated with letrozole (LT), a non-steroidal aromatase inhibitor, for 6 months. The testicular tissues were fixed in Bouin's fixative and embedded in paraffin. Immunohistochemistry with monoclonal antibodies (abs) against AR, ERa, P450arom, and polyclonal abs against ERb, TRPV1, caspase-3 was applied. Long-lasting estradiol deficiency, as an effect of LT treatment, produced changes in the morphology of testis and altered the expression of the studied receptors in cells of the seminiferous tubules and rate of cell apoptosis. The immunostaining for AR was found in the nuclei of Sertoli cells and the cytoplasm of spermatogonia and spermatocytes in III-IV stages of the seminiferous epithelium cycle. The intensity of staining for P450arom was lower in the testis of LT-treated rats as compared to control animals. The immunofluorescence of ERa and ERb was observed exclusively in the nuclei of Leydig cells of LT-treated rats. There were no changes in localization of TRPV1, however, the intensity of reaction was stronger in germ cells of the seminiferous epithelium after LT treatment. The apoptosis in both groups of animals was observed within the population of spermatocytes and spermatids in II and III stages of the seminiferous epithelium cycle. In testis of LT-treated rats the immunoexpression of caspase-3 was additionally found in the germ cells in I and IV stages, and Sertoli, myoid and Leydig cells. In conclusion, our results underline the important role of letrozole treatment in the proper function of male reproductive system, and additionally demonstrate that hormonal imbalance can produce the morphological abnormalities in testis.

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“…Furthermore, studies have shown that estrogens are involved in several aspects of rodent spermatogenesis: proliferation of gonocytes and spermatogonia, control of apoptosis, and in some steps of spermiogenesis (for reviews, O'Donnell et al (2001) and Carreau & Hess (2010)). Cytochrome P450 aromatase has been shown to be expressed in the adult rat testis at the transcript and protein levels and to be biologically active in adult Leydig and germ cells (for review, Carreau et al (2011)). Moreover, cytochrome P450 aromatase mRNA levels are regulated according to age, cell type, and stages of the seminiferous epithelium .…”

Section: Introductionmentioning

confidence: 99%

17β-Estradiol regulates cyclin A1 and cyclin B1 gene expression in adult rat seminiferous tubules

Bois¹,

Delalande²,

Bouraïma-Lelong³

et al. 2011

Journal of Molecular Endocrinology

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Spermatogenesis, which is the fundamental mechanism allowing male gamete production, is controlled by several factors, and among them, estrogens are likely concerned. In order to enlighten the potential role of estrogen in rat spermatogenesis, seminiferous tubules (ST) from two groups of seminiferous epithelium stages (II-VIII and IX-I) were treated with either 17b-estradiol (E 2 ) agonists or antagonists for estrogen receptors (ESRs). In this study, we show that cyclin A1 and cyclin B1 gene expression is controlled by E 2 at a concentration of 10 K9 M only in stages IX-I. This effect is mimicked by a treatment with the G-protein coupled estrogen receptor (GPER) agonist G1 and is abolished by treatment with the ESR antagonist ICI 182 780. Moreover, using letrozole, a drug that blocks estrogen synthesis, we demonstrate that these genes are under the control of E 2 within rat ST. Thus, germ cell differentiation may be regulated by E 2 which acts through ESRs and GPER, expressed in adult rat ST.

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Estrogen signaling in testicular cells

Cited by 63 publications

References 51 publications

17β-estradiol signaling and regulation of Sertoli cell function

17β-estradiol signaling and regulation of Sertoli cell function

The immunoexpression of androgen receptor, estrogen receptors alpha and beta, vanilloid type 1 receptor and cytochrome p450 aromatase in rats testis chronically treated with letrozole, an aromatase inhibitor

17β-Estradiol regulates cyclin A1 and cyclin B1 gene expression in adult rat seminiferous tubules

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