Estrogenic and safety evaluation of root extract of Millettia aboensis as a potential plant derived alternative for hormone replacement therapy

Ajaghaku, Amara Anwuchaepe; Ajaghaku, Daniel Lotanna; Onyegbule, Felix A.; Okoye, Festus Basden Chiedu

doi:10.1016/j.sajb.2021.03.040

Cited by 3 publications

(7 citation statements)

References 25 publications

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“…Its estrogenic effective and toxic doses have also been previously reported in our earlier study (Anwuchaepe et al, 2019). We have equally reported its osteoprotective effect in ovariectomy-induced osteoporosis (Ajaghaku et al, 2021a). To explore further beneficial effects of this plant in other menopause-associated disease conditions, this study examined the effects of a phytoestrogen-rich fraction of M. aboensis on some predictors of cardiovascular risk in an ovariectomized rat model of menopause.…”

Section: Introductionmentioning

confidence: 64%

“…Extraction and fractionation were carried out as previously reported (Ajaghaku et al, 2021a(Ajaghaku et al, , 2021b. Using a soxhlet extractor, methanol was used to extract the powdered M. aboensis root, this was filtered and concentrated in vacuo using a rotary evaporator (RE300 Model, United Kingdom) at 50 °C.…”

Section: Extraction and Fractionationmentioning

confidence: 99%

“…Dereplication studies on ethyl acetate fraction (phytoestrogen-rich fraction) chromatographic sub-fractions were carried out using HPLC-DAD and HPLC-ESI-MS analyses for the detection and identification of the phytoestrogens following previously reported protocols by Ajaghaku et al (2021a).…”

Section: High-performance Liquid Chromatography (Hplc) Analysismentioning

confidence: 99%

“…Group 1 received 5 ml/kg of distilled water (negative control); Group 2 received 1 mg/kg of estradiol valerate (positive control); Group 3 received 5 ml/kg of distilled water (sham-operated control); Groups 4 and 5 received 200 and 400 mg/kg of the phytoestrogen-rich fraction, respectively. Dose selection was based on our previous study on the estrogenic effective dose (ED50) of the root extract of M. aboensis (Ajaghaku et al, 2021a). On the 31 st day of treatment, blood samples were collected from all the animals through retro-orbital puncture for the estimation of serum lipid profile, antioxidant activities, and platelet count.…”

Section: Experimental Designmentioning

confidence: 99%

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Effect of phytoestrogen-rich fraction of Millettia aboensis on lipid profile, oxidative stress, and platelet count in ovariectomized rat model of menopause

Anwuchaepe Ajaghaku,

Ajaghaku,

Onyegbule

et al. 2023

ijpbp

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Milletia aboensis has long-standing ethnopharmacological indications for the treatment of symptoms and diseases related to menopause. This investigation examined the effects of its phytoestrogen-rich fraction on some predictors of cardiovascular risk in an ovariectomized rat model of menopause. In vitro, antioxidant activity was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) test. Ovariectomy was used to trigger menopause by surgically removing the ovaries under anesthesia. After recovery, the animals were treated orally with the phytoestrogen-rich fraction (PERF) of M. aboensis daily for 30 days. Blood samples were obtained for the estimation of serum lipid profile, antioxidant enzyme activities, lipid peroxidation, and platelet count. The PERF showed the strongest inhibition of DPPH radical with an IC50 value of 19.65 µg/ml. At 200 and 400 mg/kg, the PERF dose-dependently showed significant increase in high-density lipoprotein (HDL) (42.86 and 43.05 mg/dl) and a significant (p < 0.05) decrease in low-density lipoprotein (LDL) levels (3.78 and 1.24 mg/dl) compared with the ovariectomized (OVX) control (31.82 and 20.32 mg/dl, respectively). Being dose-dependently, the PERF increased catalase (CAT) and superoxide dismutase (SOD) enzyme activities as well as significantly reduced malondialdehyde (MDA) levels compared to OVX control. At 200 and 400 mg/kg, the PERF restored platelet count with values (316.54 and 343.3 103/IU, respectively) close to that of sham-operated control (365.17 103/IU). The ability of M. aboensis to reverse lipid abnormalities associated with ovariectomy coupled with its reduced platelet count, and antioxidant effects make it a potential therapeutic phytoestrogen remedy for the management of cardiovascular complications associated with estrogen deficiency.

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Section: Introductionmentioning

confidence: 64%

Section: Extraction and Fractionationmentioning

confidence: 99%

Section: High-performance Liquid Chromatography (Hplc) Analysismentioning

confidence: 99%

Section: Experimental Designmentioning

confidence: 99%

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Effect of phytoestrogen-rich fraction of Millettia aboensis on lipid profile, oxidative stress, and platelet count in ovariectomized rat model of menopause

Anwuchaepe Ajaghaku,

Ajaghaku,

Onyegbule

et al. 2023

ijpbp

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“…Rechtman et al, (2010) explains that there is an increase in the number of chicken eggs when it is given turmeric powder before entering puberty, since it is an indication of phytoestrogen activity in increasing the synthesis of vitolegenin (Raza et al, 2022). In the presence of phytoestogen activity in increasing vitoleginin synthesis, it means turmeric can aid estrogenic performance in poultry (Ajaghaku et al, 2021). Phytoestrogens can be bound to estrogen receptors.…”

Section: Figure 1 Village Chicken Egg Weights That Have Been Given Tu...mentioning

confidence: 99%

Performance of Gallus gallus domesticus Induced by Turmeric (Curcuma longa L.) Powder before Puberty

Putra

Bare²,

Mansur³

et al. 2022

J. Edubiotik

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The lack of use of turmeric powder by farmers to increase the production of native chicken eggs such as increasing egg weight. The purpose of the research was to determine the effect of turmeric powder supplement (Curcuma longa L.) on the weight of native chicken eggs (Gallus gallus domesticus) that had been induced on standard feed before entering the pebertas period. The design of this study is to use a complete randomized design with a total of 3 treatments. Treatment consists of the first; chicken that is not given turmeric powder supplement (P0). Second; native chickens are given turmeric powder supplements at a dose of 216 mg/ chicken /day (P1). Third; is a native chicken given turmeric powder supplement with a dose of 324 mg/ chicken /day. Egg weight data is obtained from the results of measurements made every day when all chickens have laid eggs. One month's measurement data is taken from 28 days of measurement. All measurement data is analyzed with analysis of variance (ANOVA). If the data is influential, it is then tested with a Duncan test with a trust of 95 % (α>0.05). Supplementation of turmeric powder mixed in daily standard feed before entering puberty can have an effect on increasing the weight of free-range chicken eggs (Gallus gallus domesticus). There was a significant difference in free-range chickens that were given turmeric powder supplements at a dose of 324 mg/ chicken /day (483.6 mg) compared to chickens that did not receive turmeric powder supplements (405.4 mg).

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Osteoprotective Effect of Phytoestrogen Rich Fraction of Ochna schweinfurthiana Stembark Extract on Ovariectomized Animal Model of Menopause

2023

JNSR

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Osteoporosis is one of the major disease conditions associated with estrogen deficiency in postmenopausal women. This study evaluated the osteoprotective effect of phytoestrogen rich fraction (PERF) of O. schweinfurthiana back extract on ovariectomized animal model of menopause. The methanol plant extract was partitioned into n-hexane, ethyl acetate, butanol and water fraction using liquid-liquid fractionation. The phytoestrogens in the fractions were quantified using genistein as standard. Ovariectomy induced osteoporosis was achieved by surgical removal of the ovaries. Body weight changes, relative uterine weight, serum estradiol, bone turnover markers (Alkaline phosphatase -ALP and Acid Phosphatase -ACP), bone mineral content and density as well as lipid peroxidation assays were used to establish ability of ovariectomy to induce osteoporosis and dose response effect of the PERF. Water fraction showed the highest content of phytoestrogen which was more than 10 fold higher than the content of other fractions. Ovariectomy induced weight gain was significantly (P<0.05) lowered by PERF compared to vehicle control. Significant (P<0.05) increase in serum estradiol following treatment with PERF produced corresponding uterotrophic effect with median effective dose (ED50) of 197 mg/kg. osteoprotective effect of PERF was evident from the significant (P<0.05) increases in both bone mineral content and density which corresponded with reduction in bone turnover markers. Ovariectomy induced oxidative stress was reduced by PERF with ED50 of 716 mg/kg. O. schweinfurthiana exhibited osteoprotective effect that may have been mediated by downstream signaling effects through estrogen receptor.

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Estrogenic and safety evaluation of root extract of Millettia aboensis as a potential plant derived alternative for hormone replacement therapy

Cited by 3 publications

References 25 publications

Effect of phytoestrogen-rich fraction of Millettia aboensis on lipid profile, oxidative stress, and platelet count in ovariectomized rat model of menopause

Effect of phytoestrogen-rich fraction of Millettia aboensis on lipid profile, oxidative stress, and platelet count in ovariectomized rat model of menopause

Performance of Gallus gallus domesticus Induced by Turmeric (Curcuma longa L.) Powder before Puberty

Osteoprotective Effect of Phytoestrogen Rich Fraction of Ochna schweinfurthiana Stembark Extract on Ovariectomized Animal Model of Menopause

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