Estrogenic Compounds Compatible with a Conditional Gene Expression System for the Phytopathogenic Fungus Fusarium graminearum

Lee, Jung Kwan; Son, Hokyoung; Lee, Yin Won

doi:10.5423/ppj.2011.27.4.349

Cited by 5 publications

(5 citation statements)

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“…The zearalenone (ZEA)-inducible promoter ( P zear ) is the only available conditional gene expression system for F. graminearum 28 . Furthermore, the potential for the inexpensive reagent β-estradiol (β-est) to substitute for ZEA in this conditional gene expression system has been demonstrated, thereby facilitating the utilisation of this system in high-throughput studies 29 .…”

Section: Resultsmentioning

confidence: 99%

Heat shock protein 90 is required for sexual and asexual development, virulence, and heat shock response in Fusarium graminearum

Bui

Lee

Lim

et al. 2016

Sci Rep

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Eukaryotic cells repress global translation and selectively upregulate stress response proteins by altering multiple steps in gene expression. In this study, genome-wide transcriptome analysis of cellular adaptation to thermal stress was performed on the plant pathogenic fungus Fusarium graminearum. The results revealed that profound alterations in gene expression were required for heat shock responses in F. graminearum. Among these proteins, heat shock protein 90 (FgHsp90) was revealed to play a central role in heat shock stress responses in this fungus. FgHsp90 was highly expressed and exclusively localised to nuclei in response to heat stress. Moreover, our comprehensive functional characterisation of FgHsp90 provides clear genetic evidence supporting its crucial roles in the vegetative growth, reproduction, and virulence of F. graminearum. In particular, FgHsp90 performs multiple functions as a transcriptional regulator of conidiation. Our findings provide new insight into the mechanisms underlying adaptation to heat shock and the roles of Hsp90 in fungal development.

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Section: Resultsmentioning

confidence: 99%

Heat shock protein 90 is required for sexual and asexual development, virulence, and heat shock response in Fusarium graminearum

Bui

Lee

Lim

et al. 2016

Sci Rep

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“…The three amplicons were mixed in a 1 : 1 : 3 molar ratio, fused in a second round of PCR, and used as a template to generate split markers with the new nested primer sets (Table S1). The amplified products were added into the protoplasts of wild‐type F. graminearum strains for transformation (Kim et al ., ; Lee et al ., ).…”

Section: Methodsmentioning

confidence: 97%

Functional analyses of individual mating-type transcripts atMATloci inFusarium graminearumandFusarium asiaticum

Kim

Cho

Lee

et al. 2012

FEMS Microbiol Lett

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Members of the Fusarium graminearum species (Fg) complex, which are homothallic ascomycetous species, carry two opposite mating-type (MAT) loci in a single nucleus for controlling sexual development. We investigated the roles of three (MAT1-1-1, MAT1-1-2, and MAT1-1-3) and two (MAT1-2-1 and MAT1-2-3) transcripts located at both loci in representative Fg complex species (F. graminearum and Fusarium asiaticum). In self-fertile F. graminearum strains, the transcript levels of MAT1-1-1, MAT1-2-1, and MAT1-2-3 peaked 2 days after sexual induction (dai) and then remained high until 12 dai, whereas MAT1-1-2 and MAT1-1-3 transcripts reached peak levels between 4 and 8 dai. In contrast, all of the MAT transcripts in self-sterile F. asiaticum strains accumulated at much lower levels than those in F. graminearum during the entire time. Targeted gene deletions confirmed that MAT1-1-1, MAT1-1-2, MAT1-1-3, and MAT1-2-1 were essential for self-fertility in F. graminearum, but MAT1-2-3 was not. All MAT-deleted strains (except ΔMAT1-2-3) produced recombinant perithecia when outcrossed to a self-fertile strain. These results indicate that developmental up-regulation of the individual MAT genes in both a proper fashion and quantity is critical for sexual development, and that alterations in the gene expression could be attributed to the variation in self-sterility among the Fg complex.

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“…We therefore introduced conditional gene expression to those genes and confirmed the phenotype. In F. graminearum , zearalenone-inducible promoter ( P ZEAR ) was used for conditional gene expression and β-estradiol can substitute for zearalenone ( 61 , 62 ). The promoter of each gene was replaced with P ZEAR and the replacement was confirmed by Southern blot hybridization (Fig.…”

Section: Resultsmentioning

confidence: 99%

A combined transcriptomic and physiological approach to understanding the adaptive mechanisms to cope with oxidative stress in Fusarium graminearum

Park,

Lee,

Moon

et al. 2023

Microbiol Spectr

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In plant-pathogen interactions, oxidative bursts are crucial for plants to defend themselves against pathogen infections. Rapid production and accumulation of reactive oxygen species kill pathogens directly and cause local cell death, preventing pathogens from spreading to adjacent cells. Meanwhile, the pathogens have developed several mechanisms to tolerate oxidative stress and successfully colonize plant tissues. In this study, we investigated the mechanisms responsible for resistance to oxidative stress by analyzing the transcriptomes of six oxidative stress-sensitive strains of the plant pathogenic fungus Fusarium graminearum . Weighted gene co-expression network analysis identified several pathways related to oxidative stress responses, including the DNA repair system, autophagy, and ubiquitin-mediated proteolysis. We also identified hub genes with high intramodular connectivity in key modules and generated deletion or conditional suppression mutants. Phenotypic characterization of those mutants showed that the deletion of FgHGG4 , FgHGG10 , and FgHGG13 caused sensitivity to oxidative stress, and further investigation on those genes revealed that transcriptional elongation and DNA damage responses play roles in oxidative stress response and pathogenicity. The suppression of FgHGL7 also led to hypersensitivity to oxidative stress, and we demonstrated that FgHGL7 plays a crucial role in heme biosynthesis and is essential for peroxidase activity. This study increases the understanding of the adaptive mechanisms to cope with oxidative stress in plant pathogenic fungi. IMPORTANCE Fungal pathogens have evolved various mechanisms to overcome host-derived stresses for successful infection. Oxidative stress is a representative defense system induced by the host plant, and fungi have complex response systems to cope with it. Fusarium graminearum is one of the devastating plant pathogenic fungi, and understanding its pathosystem is crucial for disease control. In this study, we investigated adaptive mechanisms for coping with oxidative stress at the transcriptome level using oxidative stress-sensitive strains. In addition, by introducing genetic modification technique such as CRISPR-Cas9 and the conditional gene expression system, we identified pathways/genes required for resistance to oxidative stress and also for virulence. Overall, this study advances the understanding of the oxidative stress response and related mechanisms in plant pathogenic fungi.

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Estrogenic Compounds Compatible with a Conditional Gene Expression System for the Phytopathogenic Fungus Fusarium graminearum

Cited by 5 publications

References 24 publications

Heat shock protein 90 is required for sexual and asexual development, virulence, and heat shock response in Fusarium graminearum

Heat shock protein 90 is required for sexual and asexual development, virulence, and heat shock response in Fusarium graminearum

Functional analyses of individual mating-type transcripts atMATloci inFusarium graminearumandFusarium asiaticum

A combined transcriptomic and physiological approach to understanding the adaptive mechanisms to cope with oxidative stress in Fusarium graminearum

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