Estrogenic isoflavones in rodent diets

Degen, Gisela H.; Janning, Petra; Diel, Patrick; Bolt, Hermann M.

doi:10.1016/s0378-4274(02)00009-7

Cited by 115 publications

(98 citation statements)

References 38 publications

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“…1, Table 1), although highly www.scienceasia.org variable, was comparable to those reported in the US and German rodent diets (10-54 mg/100 g of diet) 14 . The major isoflavones found in soybean-based rodent diets were the glycoside isoflavones (daidzin and genistin) which accounted for 82-86% of the total isoflavones.…”

Section: Isoflavone Content Of Rodent Dietssupporting

confidence: 78%

“…However, the diets fed to experimental rodents usually contain soybean products as a protein source. A number of laboratories in the US and Europe have reported that commercially available rodent dietary formulations contain variable but significant levels of phytoestrogens, especially daidzein and genistein [14][15][16][17][18] . They therefore deliver large daily doses of isoflavones to experimental rodents.…”

Section: Introductionmentioning

confidence: 99%

“…This was shown by the isoflavone levels in the serum of adult rats (2613 ± 873 ng/ml) and mice (2338 ± 531 ng/ml) exceeding the animals' endogenous estrogen levels by 30 000-60 000 times 19 . Dagen et al 14 conducted an experiment to evaluate the estrogenic effect of genistein in ovariectomized (OVX) rats by determining the uterine weight. They reported that when given the same dose of genistein treatment, rats fed with soybean diet exhibited a larger uterine weight than rats fed with a phytoestrogenfree diet.…”

Section: Introductionmentioning

confidence: 99%

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Urasopon¹,

Hamada²,

Asaoka³

et al. 2008

ScienceAsia

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ABSTRACT:Pueraria mirifica or White Kwao Krua has been extensively studied for its estrogenic effects on reproductive organs and bones using rodents as experimental animals. Commercial rodent diets are usually formulated with soybean products and therefore deliver a high dose of isoflavone phytoestrogens. Using high performance liquid chromatography, we determined the quantities of five major isoflavones (puerarin, daidzin, genistin, daidzein, and genistein) in five lots of standard rodent diets, a soybean-free diet, and two lots of P. mirifica 'Wichai-III'. The concentrations of total isoflavones were 38.6-72.4 mg/100 g in the standard rodent diets, 6.1 mg/100 g in the soybean-free diet, and 123.2-157.3 mg/100 g in the P. mirifica. While absent in the rodent diets, puerarin accounted for about half of the isoflavone content in P. mirifica. The levels of genistein and genistin in P. mirifica were very low compared to the level found in the standard rodent diets. Given the same dose of 50 mg/kg BW/day of P. mirifica for 14 days, rats fed with standard rodent diet showed a significantly higher percentage of cornified cells than those fed with soybean-free diet. These findings suggest the potential presence of phytoestrogens in standard rodent diets and its liability to be a confounding factor in estrogenic or phytoestrogenic research.

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Section: Isoflavone Content Of Rodent Dietssupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Urasopon¹,

Hamada²,

Asaoka³

et al. 2008

ScienceAsia

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“…In these standard chow diets, soy bean meal and grains are commonly used (Nutrient Requirements of Laboratory Animals, 1995). Both soybean and grains are rich sources of phytoestrogens, isoflavones and lignans, respectively (Thigpen et al, 1999;Brown and Setchell, 2001;Degen et al, 2002;Saarinen et al, 2002). Therefore, it is recommended to avoid laboratory chows based on crude plant components in studies designed to test the estrogenic potency of dietary compounds.…”

Section: Testing Estrogenicity Of Food and Testing Estrogenicity Of Fmentioning

confidence: 99%

Tools to evaluate estrogenic potency of dietary phytoestrogens:A consensus paper from the EU Thematic Network “Phytohealth” (QLKI-2002-2453)

Saarinen

Bingham²,

Lorenzetti

et al. 2006

Genes Nutr

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“…Several studies demonstrated that some commonly used outbred rats and mice are less responsive to oestrogenic substances than certain inbred mouse and rat strains, at various oestrogensensitive endpoints. With regards to diet, many laboratory animal feeds exhibit oestrogenic activity (6,15,31). It was found that soy isoflavones present in feed at high amounts interfere with the value of animal models and with experimental results (10).…”

Section: Discussionmentioning

confidence: 99%

Usefulness of immature golden hamster (Mesocricetus auratus) as a model for uterotrophic assay

Radko

Minta

Jasik

et al. 2015

Bulletin of the Veterinary Institute in Pulawy

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The present study assessed the sensitivity of immature hamster uterotrophic assay to reference oestrogen agonists/antagonists in order to develop a sensitive model for evaluation of endocrine-active compounds in diets. After performing a baseline for control animals, the sensitivity of immature females (postnatal day 18) to reference compounds was evaluated in a three-day uterotrophic assay. The absolute and adjusted dry uterine weights, fold induction over control for absolute wet uterine weight, and wet uterine weight/body weight ratio (%) were used as endpoints. The significantly active doses for reference oestrogens were as follows: 0.6 µg/kg for 17α-ethinyloestradiol (s.c.); 1 µg/kg/day (s.c.) and 40 µg/kg (p.o.) for diethylstilboestrol; 40 mg/kg (s.c.) and 160 mg/kg (p.o.) for bisphenol A. Co-treatment with tamoxifen at a dose of 1 mg/kg significantly antagonised the uterotrophic effect induced by 1 µg/kg 17α-ethinyloestradiol, and showed the attenuated proliferative effect in histopathological examination. We found immature hamster uterotrophic assay as a sensitive model that could be a good alternative to the rat assay.

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Estrogenic isoflavones in rodent diets

Cited by 115 publications

References 38 publications

Untitled

Untitled

Tools to evaluate estrogenic potency of dietary phytoestrogens:A consensus paper from the EU Thematic Network “Phytohealth” (QLKI-2002-2453)

Usefulness of immature golden hamster (Mesocricetus auratus) as a model for uterotrophic assay

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