Estrogenic modulation of CYP3A38, CYP3A40, and CYP19 in mature male medaka (Oryzias latipes)

Kashiwada, Shosaku; Kameshiro, Maiko; Tatsuta, Haruki; Sugaya, Yoshio; Kullman, Seth W.; Hinton, David E.; Goka, Koichi

doi:10.1016/j.cbpc.2007.01.009

Cited by 18 publications

(6 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In chicken, DHRS12 has been identified for its plausible function in yolk and follicle developments [37]. CYP3A40, a cytochrome P450 member, hydroxylates testosterone to regulate the metabolism of hormone in medaka [38]. Our study showed that the expression of dhrs11 and cyp3a40 was significantly increased after MT implantation, while the expression of dhrs12 was decreased during spermatogenesis.…”

Section: Discussionmentioning

confidence: 59%

Transcriptome profiling of laser-captured germ cells and functional characterization of zbtb40 during 17alpha-methyltestosterone-induced spermatogenesis in orange-spotted grouper (Epinephelus coioides)

Yang

Zhong

et al. 2020

BMC Genomics

View full text Add to dashboard Cite

Background: Spermatogenesis is an intricate process regulated by a finely organized network. The orange-spotted grouper (Epinephelus coioides) is a protogynous hermaphroditic fish, but the regulatory mechanism of its spermatogenesis is not well-understood. In the present study, transcriptome sequencing of the male germ cells isolated from orange-spotted grouper was performed to explore the molecular mechanism underlying spermatogenesis. Results: In this study, the orange-spotted grouper was induced to change sex from female to male by 17alphamethyltestosterone (MT) implantation. During the spermatogenesis, male germ cells (spermatogonia, spermatocytes, spermatids, and spermatozoa) were isolated by laser capture microdissection. Transcriptomic analysis for the isolated cells was performed. A total of 244,984,338 clean reads were generated from four cDNA libraries. Real-time PCR results of 13 genes related to sex differentiation and hormone metabolism indicated that transcriptome data are reliable. RNA-seq data showed that the female-related genes and genes involved in hormone metabolism were highly expressed in spermatogonia and spermatozoa, suggesting that these genes participate in the spermatogenesis. Interestingly, the expression of zbtb family genes showed significantly changes in the RNA-seq data, and their expression patterns were further examined during spermatogenesis. The analysis of cellular localization of Eczbtb40 and the co-localization of Eczbtb40 and Eccyp17a1 in different gonadal stages suggested that Eczbtb40 might interact with Eccyp17a1 during spermatogenesis. Conclusions:Our study, for the first time, investigated the transcriptome of the male germ cells from orangespotted grouper, and identified functional genes, GO terms, and KEGG pathways involved in spermatogenesis. Furthermore, Eczbtb40 was first characterized and its role during spermatogenesis was predicted. These data will contribute to future studies on the molecular mechanism of spermatogenesis in teleosts.

show abstract

Section: Discussionmentioning

confidence: 59%

Transcriptome profiling of laser-captured germ cells and functional characterization of zbtb40 during 17alpha-methyltestosterone-induced spermatogenesis in orange-spotted grouper (Epinephelus coioides)

Yang

Zhong

et al. 2020

BMC Genomics

View full text Add to dashboard Cite

show abstract

“…While there were no significant variations in concentrations of 17β-E2 between males from p , p ′-DDE exposure groups and the control group, the plasma 17β-E2 levels in males from the 0.005, 0.05, 0.5, and 5 μg/L o , p ′-DDT exposure groups were 1.1 ± 0.2, 0.9 ± 0.2, 1.3 ± 0.3, and 2.6 ± 1.0 ng/mL, respectively, significantly higher than those in males from the control group (0.4 ± 0.3 ng/mL) (Figure ). The increase in expression of CYP19a and elevated levels of plasma 17β-E2 were also observed in male medaka after exposure to another estrogenic compound (EE2) . Thus, the endogenously induced 17β-E2 in male fish from o , p ′-DDT groups could partly contribute to its relatively high estrogenic effects which induce VTG and CHG , and intersex in male medaka.…”

Section: Resultsmentioning

confidence: 94%

p,p′-DDE Induces Gonadal Intersex in Japanese Medaka (Oryzias latipes) at Environmentally Relevant Concentrations: Comparison with o,p′-DDT

Sun

Wang

Peng

et al. 2015

Environ. Sci. Technol.

View full text Add to dashboard Cite

Previous studies have reported high body burdens of dichlorodiphenyltrichloroethane (DDT) and its metabolites in wild fishes worldwide. This study evaluated the adverse effects of 1,1-dichloro-2,2-bis (p-chlorophenyl)-ethylene (p,p'-DDE) and o,p'-DDT on gonadal development and reproduction by exposing transgenic Japanese medaka (Oryzias latipes) from hatch for 100 days. While both p,p'-DDE and o,p'-DDT induced intersex in male medaka, the lowest observable effective concentration (LOEC) of o,p'-DDT was 57.7 ng/g ww, about 5-fold lower than that (272 ng/g ww) of p,p'-DDE. Since LOECs of both chemicals were comparable to the body concentrations in wild fish, DDT contamination would likely contribute to the occurrence of intersex observed in wild fish. Exposure to o,p'-DDT resulted in much higher expression of vitellogenin in liver of males than p,p'-DDE, accordant with the higher potency of o,p'-DDT than p,p'-DDE to induce intersex. This phenomenon could be partly explained by the significantly elevated levels of 17β-estradiol in plasma of males exposed to o,p'-DDT, in addition to its estrogenic activity via the estrogen receptor. Significantly lower fertilization (p = 0.006) and hatchability (p = 0.019) were observed in the 13 intersex males. This study for the first time demonstrated the induction of intersex and reproductive effects of p,p'-DDE and o,p'-DDT at environmentally relevant concentrations.

show abstract

“…Based on previous estrogen treatment studies, the dose of EE2 (10 ug/g body weight) was considered as a relative high concentration [33], [52], [53]. Under high dose of EE2 treatment, testis development and spermatogenesis is impaired, including inhibition of spermatogonia proliferation and differentiation, apoptosis of undifferentiated spermatogonia and reduction of seminal fluid volume [31], [54]–[56].…”

Section: Discussionmentioning

confidence: 99%

Sex-Biased miRNAs in Gonad and Their Potential Roles for Testis Development in Yellow Catfish

Jing

Liu

et al. 2014

PLoS ONE

View full text Add to dashboard Cite

Recently, YY super-male yellow catfish had been created by hormonal-induced sex reversal and sex-linked markers, which provides a promising research model for fish sex differentiation and gonad development, especially for testis development. MicroRNAs (miRNAs) have been revealed to play crucial roles in the gene regulation and gonad development in vertebrates. In this study, three small RNA libraries constructed from gonad tissues of XX female, XY male and YY super-male yellow catfish were sequenced. The sequencing data generated a total of 384 conserved miRNAs and 113 potential novel miRNAs, among which 23, 30 and 14 miRNAs were specifically detected in XX ovary, XY testis, and YY testis, respectively. We observed relative lower expression of several miR-200 family members, including miR-141 and miR-429 in YY testis compared with XY testis. Histological analysis indicated a higher degree of testis maturity in YY super-males compared with XY males, as shown by larger spermatogenic cyst, more spermatids and fewer spermatocytes in the spermatogenic cyst. Moreover, five miR-200 family members were significantly up-regulated in testis when treated by 17α-ethinylestradiol (EE2), high dose of which will impair testis development and cell proliferation. The down-regulation of miR-141 and 429 coincides with the progression of testis development in both yellow catfish and human. At last, the expression pattern of nine arbitrarily selected miRNAs detected by quantitative RT-PCR was consistent with the Solexa sequencing results. Our study provides a comprehensive miRNA transcriptome analysis for gonad of yellow catfish with different sex genotypes, and identifies a number of sex-biased miRNAs, some of that are potentially involved in testis development and spermatogenesis.

show abstract

Estrogenic modulation of CYP3A38, CYP3A40, and CYP19 in mature male medaka (Oryzias latipes)

Cited by 18 publications

References 54 publications

Transcriptome profiling of laser-captured germ cells and functional characterization of zbtb40 during 17alpha-methyltestosterone-induced spermatogenesis in orange-spotted grouper (Epinephelus coioides)

Transcriptome profiling of laser-captured germ cells and functional characterization of zbtb40 during 17alpha-methyltestosterone-induced spermatogenesis in orange-spotted grouper (Epinephelus coioides)

p,p′-DDE Induces Gonadal Intersex in Japanese Medaka (Oryzias latipes) at Environmentally Relevant Concentrations: Comparison with o,p′-DDT

Sex-Biased miRNAs in Gonad and Their Potential Roles for Testis Development in Yellow Catfish

Contact Info

Product

Resources

About