Estrous Stage- and Animal Age-Independent Superovulation in the BrlHan:WIST@Jcl(GALAS) Rat

Sotomaru, Yusuke; Kamisako, Tsutomu; Hioki, Kyoji

doi:10.1538/expanim.54.137

Cited by 7 publications

(13 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In most outbred and inbred rats, superovulation can be successfully induced using immature females [4,30]. However, some Wistar rats such as Wistar-Imamichi and BrlHan:WIST@Jcl(GALAS) respond to superovulatory treatments independent of age and the estrous cycle [15,21,26,33]. Our study indicates that the superovulatory response in WMN/Nrs is quite different from that reported in other strains of rats.…”

Section: Discussionmentioning

confidence: 58%

“…In mice, superovulatory techniques are well established: e.g., intraperitoneal injection of PMSG and hCG. Although various superovulatory methods for rats have been described, such as continuous injection of gonadotropin [1] and immunization against inhibin [12], gonadotropin injection used successfully in mice, has also worked well in Wistar rats [21,26,33]. In most outbred and inbred rats, superovulation can be successfully induced using immature females [4,30].…”

Section: Discussionmentioning

confidence: 99%

“…Theses amounts of gonadotropins were determined in a preliminary study in which the ovulatory response after injection of the above amounts (8/8, 100%) to 8-weekold females was better than that induced by 150 IU/Kg PMSG and 75 IU/Kg hCG (6/8, 75%), which used in previous studies [26,33]. When necessary, the estrous cycle was examined by examining vaginal smears between 11:00-12:00 am.…”

Section: Hormone Treatmentmentioning

confidence: 99%

“…Among these methods, the PMSG-hCG injection regimen seems to be the most practical and economical method of inducing superovulation and may be applied to a wide range of ages in Wistar rats [15,21,26,30,33]. However, quantitative data on the actual use of this technique in rats is limited to studies of juvenile rats and a few adult strains.…”

Section: Introductionmentioning

confidence: 99%

“…Data reported for mice and hamsters are controversial, with some studies showing significant differences in quality and other showing no difference [5,6,22,23,32,34,37]. However, in rats the quality of superovulated oocytes or embryos, as indicated by developmental ability to the fetus, has not been directly compared with that obtained by natural ovulation [10,30,33,40], and such information is essential for estimating the efficiency of animal production and the normality of embryos. Thus, obtaining normative information concerning superovulation and the quality of superovulated oocytes or embryos on a strain-by-strain basis, especially for inbred rats, would be quite useful for ARTs of laboratory rats.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Superovulatory Response, Oocyte Spontaneous Activation, and Embryo Development in WMN/Nrs Inbred Rats

Kito¹,

Yano

Ohta

et al. 2010

Exp. Anim.

View full text Add to dashboard Cite

WMN/Nrs inbred rats have been widely used in radiation biology for years. However, their reproductive profile has never been examined. We examined various reproductive characteristics of WMN/Nrs inbred rats such as superovulatory response, oocytes spontaneous activation (OSA), and embryo development in vitro and in vivo. Superovulation was induced in 3-to 9-week-old females by injection of 150 IU/kg PMSG and 150 IU/Kg hCG by 48 h apart. Only 8-and 9-week-old animals superovulated averaging 31.4 and 43.9 oocytes, respectively, and superovulation did not depend on estrous cycle. Animals 3-7 weeks of age did not superovulate. Because Wistar strains have been known to show a high incidence of OSA, factors expected to affect OSA in WMN/Nrs, including the time interval of various steps from euthanasia to oocyte recovery, incubation media, estrous cycle, and anesthetic treatments, were examined. The time from animal euthanasia to oviduct excision was the only factor shown to affect OSA. We also compared in vitro and in vivo embryo developmental competence between embryos obtained by natural ovulation and superovulation. Although percent in vitro development of 2-cell embryos to blastocysts was similar for embryos obtained by natural ovulation (63.7%) and superovulation (69.7%), fetus development after oviductal transfer of 2-cell embryos was significantly lower in embryos obtained by superovulation than in those obtained by natural ovulation (60.2% vs. 87.5%, P=0.02). Our results provide important normative data regarding future applications of rat assisted reproductive technologies (ARTs) such as in vitro fertilization and cryopreservation in WMN/Nrs strain and may be applicable to other strains of laboratory rats.

show abstract

Section: Discussionmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 99%

Section: Hormone Treatmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Superovulatory Response, Oocyte Spontaneous Activation, and Embryo Development in WMN/Nrs Inbred Rats

Kito¹,

Yano

Ohta

et al. 2010

Exp. Anim.

View full text Add to dashboard Cite

show abstract

Advances in transgenic rat production

Filipiak

Saunders

2006

Transgenic Res

View full text Add to dashboard Cite

Predictable and reproducible production of transgenic rats from a standardized input of egg donors and egg recipients is essential for routine rat model production. In the course of establishing a transgenic rat service, transgenic founders were produced from three transgenes in outbred Sprague-Dawley (SD) rats and four transgenes in inbred Fischer 344 (F344) rats. Key parameters that affect transgenesis efficiency were assessed, including superovulation treatments, methods to prepare pseudopregnant recipients, and microinjection technique. Five superovulation regimens were compared and treatment with 20 IU PMSG and 30 IU HCG was selected for routine use. Four methods to prepare pseudopregnant egg recipients were compared and estrus synchronization with LHRHa and mating to vasectomized males was selected as most effective. More than 80% of eggs survived microinjection when modified pronuclear microinjection needles and DNA buffers were used. The efficiencies of transgenic production in rats and C57BL/6J (B6J) mice were compared to provide a context for assessing the difficulty of transgenic rat production. Compared to B6J mice, SD rat transgenesis required fewer egg donors per founder, fewer pseudopregnant egg recipients per founder, and produced more founders per eggs microinjected. Similar numbers of injection days were required to produce founders. These results suggest that SD rat transgenesis can be more efficient than B6J mouse transgenesis with the appropriate technical refinements. Advances in transgenic rat production have the potential to increase access to rat models.

show abstract

A study on cryoprotectant solution suitable for vitrification of rat two-cell stage embryos

Eto

Takahashi²,

Kamisako

et al. 2014

Cryobiology

View full text Add to dashboard Cite

The present study was performed to develop a suitable cryoprotectant solution for cryopreservation of rat two-cell stage embryos. First, we examined the cell permeability of several cryoprotectants; propylene glycol had the fastest permeability compared to dimethyl sulfoxide, ethylene glycol, and glycerol. Embryos were then exposed to a solution containing propylene glycol to evaluate its effects on fetal development. As the development was similar to that of fresh embryos, P10 (10% v/v propylene glycol in PB1) was used as a pretreatment solution. Next, the effects of the vitrification solution components (sucrose, propylene glycol, ethylene glycol, and Percoll) were examined by observing the vitrification status; 10% v/v propylene glycol, 30% v/v ethylene glycol, 0.3 mol sucrose, and 20% v/v Percoll in PB1 (PEPeS) was the minimum essential concentration for effective vitrification without the formation of ice crystals or freeze fractures. A new vitrification method using P10 and PEPeS was tested using rat embryos. The survival rate of vitrified embryos after exposure to P10 for 120, 300, or 600 s ranged from 95.9% to 98.3%. The fetal developmental rate ranged from 57.7% to 65.2%, which was not significantly different from that of fresh embryos. The experimental results indicated that vitrification using a combination of P10 and PEPeS was suitable for cryopreservation of rat early stage embryos.

show abstract

Estrous Stage- and Animal Age-Independent Superovulation in the BrlHan:WIST@Jcl(GALAS) Rat

Cited by 7 publications

References 13 publications

Superovulatory Response, Oocyte Spontaneous Activation, and Embryo Development in WMN/Nrs Inbred Rats

Superovulatory Response, Oocyte Spontaneous Activation, and Embryo Development in WMN/Nrs Inbred Rats

Advances in transgenic rat production

A study on cryoprotectant solution suitable for vitrification of rat two-cell stage embryos

Contact Info

Product

Resources

About