Estudio Comparativo De Técnicas De Conservación Anatómica De Especimenes De Cadaveres Ovinos en Altura, Utilizando Soluciones De Formol Y Prives

Abstract

RESUMENSe realizó el estudio comparativo de dos técnicas de conservación de espécimen animal: solución de formol al 10% y solución de PRIVES utilizando como unidad experimental a ovinos criollos procedentes del distrito de Acora efectuados en el Laboratorio de Anatomía Veterinaria y Laboratorio de Microbiología de la Facultad de Medicina Veterinaria y Zootecnia durante los meses de Agosto a Diciembre del 2013; dos ovinos fueron perfundidos con solución de formol al 10% y dos con solución de PRIVES. Se analizaron variables cualitativas de consistencia, color, olor y presencia de hongos, como el tiempo de conservación e identificación de hongos con el seguimiento y recopilación de datos a los 30, 50, 65 y 80 días, obteniéndose los siguientes resultados: La solución de PRIVES mostró mejores propiedades de conservación, manteniendo la consistencia, color y flexibilidad de los órganos con características normales en estado fresco hasta 50 días y que con Formol al 10% se observó: rigidez cambio de color y olor irritante. El tiempo de conservación del cadáver fue de 63 días con solución de Formol al 10% y de 70 días con la solución de PRIVES. Se identificaron los hongos del género Penicillium spp en especímenes conservados con Formol al 10 % y Levaduras en especímenes conservados con solución de PRIVES. Se concluye que, la solución de PRIVES conserva mejor las características macroscópicas de las estructuras anatómicas, emplear como solución alternativa al formol en el Laboratorio de Anatomía Veterinaria. COMPARATIVE STUDY TECHNIQUES OF ANATOMICAL CONSERVATION OF SHEEP CORPSES AT ALTITUDE, USING FORMALDEHYDE SOLUTION AND PRIVES ABSTRACTA comparative study of two techniques for animal specimen conservation were performed with a solution of 10% formaldehide and a Prives solution respectively. These were used on crossbred sheep as experimental units from the district of Acora. The study was performed at the Laboratory of Veterinary Anatomy and the Microbiology Laboratory at the Department of Veterinary Medicine and Zootechnics of Puno, from August to December of 2013. Two sheep were perfused with a solution of 10% formaldehyde solution and two with the Prives solution. Qualitative variables in consistency, color, odor and presence of fungus were analyzed along with time based conservation and identification of fungus. Monitoring and data collection at 30, 50, 65 and 80 days respectively, with the following results: the Prives solution showed better conservation properties, maintaining consistency, color and flexibility of normal organ characteristics up to 50 days. With 10% formaldehyde we observed stiffness, color change and irritating odors. The shelf life of the corpse was 63 days with a solution of 10% Formaldehyde and 70 days with the Prives solution. We identified Fungus of the genus Penicillium spp in specimens preserved with 10% Formaldehyde and yeasts in specimens preserved with Prives solution. We conclude that the Prives solution best retains the macroscopic characteristics of the anatomical structures, used as...

Implementation of a New Solution for the Preservation of Anatomical Specimens Made of Non-Toxic Substances

“…The resulting sheep heart specimens obtained by either technique maintained the shape and structure seen in the fresh specimens, as was determined by the study of the external and internal organ anatomy, Figures 2-4 changes in coloration are frequent when implementing techniques for anatomical preservation, the phenolated glycerin technique being the one that presented the lowest luminosity, as evidenced by a darker and lighter tone obtained using the alcohol propylene glycol technique, it should be noted that, in our experience, the anatomical pieces preserved using the phenolated glycerin technique tend to darken over time due to the use of aqueous formalin as a fixing agent, an effect also reported by Cutipa [16].…”