Estudio de colonización por Streptococcus agalactiae en gestantes extranjeras y españolas, en Elche y Comarca

Ramos, José Manuel; Milla, Alfredo Salvador; López-García, Pilar; Gutiérrez, Félix

doi:10.1016/j.eimc.2008.04.008

Cited by 6 publications

(4 citation statements)

References 9 publications

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“…The frequency of vaginal-rectal colonization varies widely among geographic areas and even in different regions of the same country. Authors such as Ramos et al (2009) suggest that; besides ethnic, geographic and social differences in the populations studied; the percentages of maternal colonization can be influenced by the different methods used to detect the bacteria [20].…”

Section: Discussion Colonization In Full-term Pregnant Womenmentioning

confidence: 99%

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Prevalence, serotypes and virulence genes of Streptococcus agalactiae isolated from pregnant women with 35–37 weeks of gestation

et al. 2021

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Background In pregnant women Streptococcus agalactiae (GBS) can be transmitted to newborn causing severe infections. It is classified into 10 serotypes (Ia, Ib, II-IX). The severity of neonatal disease is determined by the capsular serotype and virulence factors such as the polysaccharide capsule, encoded by the cps gene, protein C, which includes the Cα surface proteins (bca gene), Rib (rib gene) and Cβ (bac gene); the proteins Lmb (lmb gene), FbsB (fbsB gene), FbsA (fbsA gene), the cyl operon encoding a β-hemolysin (hylB gene), the CAMP factor (cfb gene) and the C5a peptidase (scpB gene). The aim of this work was to determine the degree of GBS colonization in pregnant women, the serotypes distribution and to investigate virulence-associated genes. Methods We worked with 3480 samples of vagino-rectal swabs of women with 35–37 weeks of gestation. The identification of the strains was carried out using conventional biochemical tests and group confirmatory serology using a commercial latex particle agglutination kit. Two hundred GBS strains were selected. Their serotype was determined by agglutination tests. The monoplex PCR technique was used to investigate nine virulence-associated genes (cps, bca, rib, bac, lmb, fbsB, fbsA, hylB and scpB). Results The maternal colonization was 9.09%. The serotypes found were: Ia (33.50%), III (19.00%), Ib (15.50%), II (14.00%), V (7.00%) and IX (5.50%). 5.50% of strains were found to be non-serotypeable (NT). The nine virulence genes investigated were detected simultaneously in 36.50% of the strains. The genes that were most frequently detected were scpB (100.00%), fbsA (100.00%), fbsB (100.00%), cylB (95.00%), lmb (94.00%) and bca (87.50%). We found associations between serotype and genes bac (p = 0.003), cylB (p = 0.02), rib (p = 0.01) and lmb (p < 0.001). Conclusions The frequency of vaginal-rectal colonization, serotypes distribution and associated virulence genes, varies widely among geographical areas. Therefore, epidemiological surveillance is necessary to provide data to guide decision-making and planning of prevention and control strategies.

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Section: Discussion Colonization In Full-term Pregnant Womenmentioning

confidence: 99%

“…The frequency of vaginal-rectal colonization varies according to geographic area, ethnic and social conditions in populations [20]. The province of Misiones has the particularity that ethnic origins are varied, mainly from European immigrants and from neighboring countries.…”

Section: Introductionmentioning

confidence: 99%

Prevalence, serotypes and virulence genes of Streptococcus agalactiae isolated from pregnant women with 35–37 weeks of gestation

et al. 2021

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“…The prevalence of GBS colonization in the vagina and/or rectum among pregnant women can vary among ethnic groups, geographical locations, and the laboratory procedures carried out on the samples (Ramos et al , 2009; Simões et al , 2007). …”

Section: Discussionmentioning

confidence: 99%

Phenotypic and genotypic characterization of Streptococcus agalactiae in pregnant women: first study in a province of Argentina

Oviedo¹,

Pegels²,

Laczeski³

et al. 2013

Braz. J. Microbiol.

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Group B Streptococcus (GBS) is the leading cause of neonatal infections. Our purpose was to characterize GBS colonization in pregnant women, current serotypes, resistance phenotypes and genes associated with virulence. In Misiones, Argentina, there are no previous data on this topic. Vaginal-rectal swabs from 3125 pregnant women were studied between 2004 and 2010. GBS strains were identified by conventional and serological methods (Phadebact Strep B Test, ETC International, Bactus AB, Sweden). Serotypes were detected using Strep-B Latex (Statens Serum Institut, Denmark). Resistance phenotypes were determined by the double-disk test. Genes were studied by PCR. Maternal colonization was 9.38%. Resistance to erythromycin was 11.6%, and the constitutive phenotype was the predominant one. Serotype Ia was the most frequent, whereas serotypes IV, VI, VII and VIII were not detected. The lmb, bca and hylB genes were detected in more than 79% of the strains. In this study, the colonization rate with GBS and the serotype distribution were compared with studies reported in other areas of the country. The high resistance to erythromycin in Misiones justifies performing antibiotic susceptibility testing. The serotype distribution, the genes encoding putative virulence factors, and the patterns of resistance phenotypes of GBS may vary in different areas. They thus need to be evaluated in each place to devise strategies for prevention.

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“…Colonization of a pregnant woman's vagina is very important, as it implies an enhanced risk of GBS being transmitted vertically to the child before or at birth, and subsequently causing infection in the newborn. In Spain, it has been reported that 10-18.5% of pregnant women are colonized by GBS [36] ; 22.76% in Tehran [37] ; 6% in Iran [38]; 19% in Poland [39]; and 20% in Taiwan [40]. To avoid this enhanced risk of vertical transmission, several diagnostic and prophylactic protocols have been proposed.…”

Section: Cdc Prophylaxis Guidelinesmentioning

confidence: 99%

Neonatal Sepsis by Bacteria: A Big Problem for Children

Soto¹

2012

Clin Microbial

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Estudio de colonización por Streptococcus agalactiae en gestantes extranjeras y españolas, en Elche y Comarca

Cited by 6 publications

References 9 publications

Prevalence, serotypes and virulence genes of Streptococcus agalactiae isolated from pregnant women with 35–37 weeks of gestation

Prevalence, serotypes and virulence genes of Streptococcus agalactiae isolated from pregnant women with 35–37 weeks of gestation

Phenotypic and genotypic characterization of Streptococcus agalactiae in pregnant women: first study in a province of Argentina

Neonatal Sepsis by Bacteria: A Big Problem for Children

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