Estudio de la interacción entre naranjo amargo y el virus de la tristeza de los cítricos

Serra, Marta Comellas

doi:10.4995/thesis/10251/7323

Cited by 4 publications

(12 citation statements)

References 233 publications

(274 reference statements)

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“…CTV infection sites, after cell to cell movement, consisted of clusters of 3 to 12 cells in the susceptible species, while in Duncan grapefruit and sour orange displayed fewer infection sites limited to single cells, indicating absence of viral movement in both cases (Folimonova et al, 2008). After the analysis of the sour orange resistance to mild, SP and T36-CTV9 CTV strains, Comellas (2009) found, similarly to Folimonova el al. (2008), a limitation of viral movement in this host.…”

supporting

confidence: 62%

“…This limitation was more accentuated for T36 and mostly complete for the mild strain. However, after two years post inoculation, both, T36 and the mild strain, accumulated in sour orange similarly to in Mexican lime revealing a transitory viral resistance (Comellas, 2009), which was also noticed by Bernet et al (2008) that using another CTV isolate and QTL-linked markers reported that CTV resistance in sour orange was distinct to that of P. trifoliata. Sour orange resistance to CTV infection could be due to the plant RNA silencing mechanism (Folimonova et al, 2008).…”

mentioning

confidence: 78%

“…In spite of the presence of these three silencing suppressors, accumulation of siRNAs in CTV-infected susceptible hosts could be 50% of the total RNAs in the plant (Ruiz-Ruiz et al, 2011). Moreover, the siRNAs accumulation is directly proportional 287 to the virus accumulation and varies depending on the CTV strain and the citrus host (Comellas, 2009). Deep sequencing analysis of siRNAs from CTV-infected plants indicated that they mainly consisted in small RNAs of 21-22 nt derived from essentially all the CTV genome (Ruiz-Ruiz et al, 2011).…”

Section: Genetic Determinants Of the Citrus Tristeza Virus Syndromesmentioning

confidence: 99%

“…In addition of the plus and minus stranded 3´ coterminal sgRNAs, the CEs corresponding to each of the ten 3´ ORFs produce a reduced amount of a set of 5´ coterminal positive-stranded sgRNAs, probably due to premature termination during the synthesis of the gRNA ). In addition, CTV generates significant amounts of low molecular-weight tristeza, LMT1 and LMT2, a two positivestranded 5´ coterminal sgRNAs population with heterogeneous 3´ termini at nt 842-854 and 744-746, respectively (Che at al., 2001), which are produced and accumulated differently (Gowda et al, 2003a;2009). LMT1 is likely created by premature termination during genomic RNA synthesis at a 5´ CE sited in the PRO I domain of replicase (Gowda et al, 2003a).…”

Section: Genome Organization and Functions Of Viral Proteinsmentioning

confidence: 99%

“…CTV resistance in Duncan grapefruit (a descent of pummelo) and in sour orange have been investigated recently (Bernet et al, 2008;Comellas, 2009;Folimonova et al, 2008). The systemic invasion of the stable virus-based vector CTV-BC5/GFP (descendent of the T36-CTV9 construct) in Duncan grapefruit and sour orange, compared to those of the susceptible hosts C. macrophylla and Mexican lime and the tolerant host Madam Vinous sweet orange, were examined (Folimonova et al, 2008).…”

mentioning

confidence: 99%

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Molecular Virology and Pathogenicity of Citrus tristeza virus

Albiach-Martı́¹

2012

Viral Genomes - Molecular Structure, Diversity, Gene Expression Mechanisms and Host-Virus Interactions

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supporting

confidence: 62%

mentioning

confidence: 78%

Section: Genetic Determinants Of the Citrus Tristeza Virus Syndromesmentioning

confidence: 99%

Section: Genome Organization and Functions Of Viral Proteinsmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Molecular Virology and Pathogenicity of Citrus tristeza virus

Albiach-Martı́¹

2012

Viral Genomes - Molecular Structure, Diversity, Gene Expression Mechanisms and Host-Virus Interactions

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Callose synthase and xyloglucan endotransglucosylase gene expression over time in Citrus × clementina and Citrus × sinensis infected with citrus tristeza virus

et al. 2023

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Citrus tristeza virus (CTV) is a virus that already caused great losses in citrus producing regions. The cell wall of plant cells plays an important role in the defence response to viruses. Following several studies indicating that cell wall enzyme transcripts of callose synthase 7 (calS7) and xyloglucan endotransglucosylase 9 (xth9) are modified during a viral infection, transcript expression of calS7 isoform x5 (calS7x5) and xth9 was evaluated over time in Citrus × sinensis ‘Valencia Late’ (VL) and Citrus × clementina ‘Fina’ (CL), infected with the severe CTV isolate T318A, by quantitative (q) PCR. qPCR analysis of healthy and CTV infected citrus was performed at 15 days, 10 months and at 31 months post-inoculation (dpi/mpi), respectively. The CTV titer, evaluated at the three time-points by qPCR, increased over time in bark tissues, with VL plants exhibiting a titer about 5 times higher than CL 31 mpi. CTV infection did not cause significant changes in calS7x5 gene expression over time in both citrus cultivars. However, CTV infection was associated with significant up-regulation of xth9 in VL compared to controls 31 mpi. This study highlights that CTV infection can affect the expression of specific cell wall-associated genes over time and that this influence was distinct for VL and CL. This study provides further insight into the CTV-citrus host interaction, with the long-term response of VL to a severe CTV isolate involving a high expression of the xth9 gene.

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Ectopic expression of the p23 silencing suppressor of Citrus tristeza virus differentially modifies viral accumulation and tropism in two transgenic woody hosts

Fagoaga¹,

Pensabene-Bellavia²,

Moreno³

et al. 2011

Molecular Plant Pathology

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Citrus tristeza virus (CTV), a phloem-restricted closterovirus infecting citrus, encodes three different silencing suppressors (p25, p20 and p23), one of which (p23) is a pathogenicity determinant that induces aberrations resembling CTV symptoms when expressed ectopically in transgenic citrus hosts. In this article, the effect of p23 ectopic expression on virus infection was examined in sweet orange (SwO), a highly susceptible host, and sour orange (SO), which severely restricts CTV cell-to-cell movement. Transgenic plants of both species ectopically expressing p23, or transformed with an empty vector, were graft inoculated with the mild CTV isolate T385 or with CTV-BC1/GFP, a clonal strain derived from the severe isolate T36 carrying the gene for the green fluorescent protein (GFP). CTV distribution in infected tissues was assessed by direct tissue blot immunoassay and fluorescence emission, and virus accumulation was estimated by quantitative real-time reverse transcriptase-polymerase chain reaction. CTV accumulation in p23-expressing and control SwO plants was similar, whereas the viral load in transgenic SO expressing p23 was 10-10(5) times higher than in the cognate control plants. Although few infection foci composed of a single cell were observed in the phloem of CTV-infected control SO, the number of foci in p23-expressing plants was higher and usually comprised two to six cells, indicating viral cell-to-cell movement. CTV was detected in mesophyll protoplasts and cells from infected SO and SwO expressing p23, but not in similar protoplasts and cells from infected control plants. Our results show that the ectopic expression of p23 enables CTV to escape from the phloem and, in addition, facilitates systemic infection of the resistant SO host. This is the first report of a viral-encoded protein that enhances virus accumulation and distribution in woody hosts.

show abstract

Estudio de la interacción entre naranjo amargo y el virus de la tristeza de los cítricos

Cited by 4 publications

References 233 publications

Molecular Virology and Pathogenicity of Citrus tristeza virus

Molecular Virology and Pathogenicity of Citrus tristeza virus

Callose synthase and xyloglucan endotransglucosylase gene expression over time in Citrus × clementina and Citrus × sinensis infected with citrus tristeza virus

Ectopic expression of the p23 silencing suppressor of Citrus tristeza virus differentially modifies viral accumulation and tropism in two transgenic woody hosts

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