Estudio del rendimiento diagnóstico de la detección de IgM específica y de la amplificación genómica de rubéola

Mosquera, Marı́a del Mar; Sanz, Juan Carlos; Echevarrı́a, Juan E.; Herranz, Nieves; Fernández, Marisa; Ory, Fernando de

doi:10.1016/s0213-005x(06)73771-5

Cited by 6 publications

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“…The diagnosis of rubella is currently made by using serological techniques, and the risk to the fetus is assessed by establishing the gestational age at the time of maternal infection. Considering the negative health consequences of RUBV on the fetus and offspring of infected mothers, an early laboratory diagnosis of recent or congenital infection through direct detection of RUBV ribonucleic acid (RNA) in clinical specimens, in addition to the detection of RUBV-specific immunoglobin M (IgM), is obviously critical [15]. …”

Section: Introductionmentioning

confidence: 99%

Rubella Seroprevalence and real-time PCR detection of RUBV among Congolese pregnant women

et al. 2017

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BackgroundRubella is an acute infectious disease caused by Rubella virus (RUBV). RUBV remains an important pathogen worldwide, causing approximately 100 000 cases of congenital rubella syndrome (CRS) every year; and the most severe consequence of rubella is teratogenicity. The aim of this study was to estimate the prevalence of RUBV IgG antibodies and determine RUBV genotypes in Congolese pregnant women in Kongo central province, Democratic Republic of Congo (DRC).MethodsThis was a prospective cross-sectional study that consisted of a laboratory analysis of blood samples from 78 pregnant women to check for the presence of RUBV IgG antibodies, and also determine RUBV genotypes in seropositive samples (using primers targeting RUBV nucleoprotein), with the use of serological and molecular methods, respectively. Participants were pregnant women attending antenatal care clinics (ANC) at two health zones of Kisantu town in DRC. They were followed-up from the first to third trimester. Those who were negative for RUBV antibodies at the initial assay (first trimester) were tested in the second and, eventually, the third trimester.ResultsAn overall rubella seroprevalence of 58.97% was observed, whereas RUBV nucleoprotein was detected in 60% of randomly selected 30 blood samples among the 46 RUBV seropositive pregnant women. Five (27.77%) of positive samples were positive for both RUBV genotypes (RV8633/9112 and RV8945/9577), whereas 11 (61.11%) of them were positive for RV8633/9112 and two (11.11%) were positive for RV8945/9577 only. Regarding rubella clinical signs and complications, two subjects (2.56%) presented with fever, whereas five pregnant women (6.41%) had experienced abortion. None (0%) of the participants has been vaccinated against RUBV.ConclusionsFindings from this study suggest that RUBV is prevalent in Congolese pregnant women. Further research is required to elucidate the molecular epidemiology of RUBV in order to design a rational rubella surveillance and control program in DRC.

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Section: Introductionmentioning

confidence: 99%

Rubella Seroprevalence and real-time PCR detection of RUBV among Congolese pregnant women

et al. 2017

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“…A strain of genotype 1J caused a large outbreak in Madrid of about 460 cases in 2004–2005, mainly affecting the Latin-American community [ 13 , 14 , 15 ], and a sporadic case was recorded in Seville (Andalusia). The associated sequences seem to be related to three others detected in Brazil in the same year (CQ329848/RioGrandedoSul.BRA/48.05) ( Fig 4 ).…”

Section: Resultsmentioning

confidence: 99%

“…For the most frequently detected 1E and 2B genotypes, Europe seems to be the most frequent origin, although it is usually difficult to establish. The genotype 1J outbreak, which occurred in Madrid in 2004–2005 [ 13 , 14 , 15 ], highlights the importance of generating sequence data about rubella genotypes for epidemiological purposes. It affected about 460 patients, predominantly Latin American immigrants, and adult Spanish males born in the early 1980s (around the time the MMR vaccine was introduced [ 15 ]), who had not been immunized in previous campaigns that had focused solely on 11-year-old girls [ 13 , 14 , 15 ].…”

Section: Discussionmentioning

confidence: 99%

“…The genotype 1J outbreak, which occurred in Madrid in 2004–2005 [ 13 , 14 , 15 ], highlights the importance of generating sequence data about rubella genotypes for epidemiological purposes. It affected about 460 patients, predominantly Latin American immigrants, and adult Spanish males born in the early 1980s (around the time the MMR vaccine was introduced [ 15 ]), who had not been immunized in previous campaigns that had focused solely on 11-year-old girls [ 13 , 14 , 15 ]. In the original description of the Madrid outbreak [ 1 , 22 ] there was no evidence of the presence of genotype 1J in either Europe or South America, and the origin of the outbreak could not be traced.…”

Section: Discussionmentioning

confidence: 99%

“…The rubella vaccine was only introduced in Latin American countries in the late 1990s, so many adult immigrants from those countries who are resident in Spain are not immunized. These circumstances led to a small outbreak in Madrid in 2003 [ 12 ], a larger one in 2004–2005 [ 13 , 14 , 15 ], and another one in Catalonia in 2005 [ 16 ] among Latin American immigrants and Spanish males born before the universalization of the vaccine. The characterization of the genotype 1J strain causing the outbreak in Madrid in 2005 [ 13 ] remains the only historical data of RUBV genotype circulation in Spain.…”

Section: Introductionmentioning

confidence: 99%

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Genetic Characterization of Rubella Virus Strains Detected in Spain, 1998-2014

et al. 2016

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The National Plan for the Elimination of Rubella was implemented in Spain in 2008 using the logistics of the National Plan for the Elimination of Measles that have been employed since year 2000. Molecular characterization of rubella virus (RUBV) is important for disease surveillance and for monitoring elimination of the disease throughout the world. We describe the first complete series of data regarding the circulation of RUBV genotypes in Spain. The 739-nucleotide fragment designated by the WHO for RUBV genotyping was sequenced in 88 selected cases collected from 1998 to 2014. Five genotypes were identified: 1E, 2B, 1J, 1I, and 1a. Genotype 1E was predominant between 1998 and 2003 but was replaced by genotype 2B, which was detected in sporadic cases in 2004, 2006, 2008, 2012, 2013 and 2014. There was an outbreak of genotype 2B in Algeciras (Andalusia) in 2008. Genotype 1J caused an outbreak in Madrid in 2004/2005 and sporadic cases in 2005 and 2007. Genotype 1I was found to have infected an immune-suppressed patient with neurological symptoms in 2008. Finally, vaccine strain RA 27/3 was detected in three sporadic cases, two of them immune-suppressed and without a recent history of vaccination. This suggests that during these years there were a series of imported sporadic cases and outbreaks, confirming the findings of epidemiological data analysis. The importation sources were generally consistent with our geographic and cultural ties, mainly with Europe (genotypes 1E, 2B, 1I) and Latin America (1J).

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Estudio observacional: evaluación de la calidad metodológica de la investigación diagnóstica en España tras la publicación de la guía STARD

Sáez¹,

Hernández-Aguado²,

Lumbreras³

2009

Medicina Clínica

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Estudio del rendimiento diagnóstico de la detección de IgM específica y de la amplificación genómica de rubéola

Cited by 6 publications

References 6 publications

Rubella Seroprevalence and real-time PCR detection of RUBV among Congolese pregnant women

Rubella Seroprevalence and real-time PCR detection of RUBV among Congolese pregnant women

Genetic Characterization of Rubella Virus Strains Detected in Spain, 1998-2014

Estudio observacional: evaluación de la calidad metodológica de la investigación diagnóstica en España tras la publicación de la guía STARD

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