ESTUDIO LECTINHISTOQUÍMICO DEL ÚTERO DE ALPACAS (Vicugna pacos) BAJO TRATAMIENTO SUPEROVULATORIO.

C., Verónica López; C., María Vásquez; L., Wilfredo Huanca; Santiani, Alexei; Barbeito, Claudio Gustavo; A., Carolina Canuzzi; M., Boris Lira; G, José Rodríguez

doi:10.15381/rivep.v25i1.8463

Cited by 2 publications

(2 citation statements)

References 19 publications

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“…In another study, UEA I presence was found in the placenta in 145, 170, and 350 days of gestation in camels and occasionally on day 283 in alpaca ( Jones et al, 2002 ). Recent investigations carried out in the alpaca uterus during superovulatory periods and normal treatment did not demonstrate any labeling of this lectin ( López C et al, 2014 ). In the equine placenta, there was no evidence of fucose staining ( Jones et al, 1999 ), which was not observed in the microvillus membrane of the human placenta as well ( Jones et al, 1997 ).…”

Section: Discussionmentioning

confidence: 89%

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Term alpaca placenta glycosylation profile and its correlation with pregnancy maintenance and fetal survival

Silva

et al. 2023

Front. Cell Dev. Biol.

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Alpaca is a South American camelid, particularly present in Peruvian highlands, where oxygen concentration and atmospheric pressure are very low. Due to this fact, gestational physiology has adapted to preserve the conceptus’ and mother’s health. In this context, several cellular and molecular features play an essential role during and at the end of gestation. Structural carbohydrates act on maternal–fetal communication, recognize exogenous molecules, and contribute to placental barrier selectivity. Therefore, this study aimed to characterize the structural carbohydrate profiles that are present in the term alpaca placenta, kept in their natural habitat of around 4,000 m height. For this propose, 12 term alpaca placentas were collected, and the material was obtained at the time of birth from camelids raised naturally in the Peruvian highlands, in the Cusco region. All placenta samples were processed for histological analysis. A lectin histochemical investigation was performed using 13 biotinylated lectins, allowing us to determine the location of carbohydrates and their intensity on a semi-quantitative scale. Our results demonstrated that during term gestation, the epitheliochorial alpaca placenta shows a high presence of carbohydrates, particularly glucose, α-linked mannose, N-acetylglucosamine β (GlcNAc), galactose (αGal), and N-acetylgalactosamine α (GalNAc), present in the trophoblast, amnion epithelium, and mesenchyme, as well as the presence of sialic acid residues and low affinity for fucose. In fetal blood capillaries, the presence of bi- and tri-antennary complex structures and α-linked mannose was predominated. In conclusion, we characterized the glycosylation profile in the term alpaca placenta. Based on our data, compared to those reported in the bibliography, we suggest that these carbohydrates could participate in the labor of these animals that survive in Peruvian extreme environments.

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Section: Discussionmentioning

confidence: 89%

“…For the negative control, sections of the same samples were used, where each lectin was replaced by PBS. The positive control was a mouse intestine sample incubated with lectins ( López C et al, 2014 ).…”

Section: Methodsmentioning

confidence: 99%

Term alpaca placenta glycosylation profile and its correlation with pregnancy maintenance and fetal survival

Silva

et al. 2023

Front. Cell Dev. Biol.

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Semen collection methods in alpacas

et al. 2017

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South American camelids, particularly alpacas, are gaining in popularity outside their native lands. Reproductive biotechnologies, such as artificial insemination or embryo transfer with cryopreserved embryos, are more complicated in these species than many others due to differences in their reproductive physiology compared to the more commonly encountered domestic livestock. This article reviews the methods currently available for obtaining semen or spermatozoa from alpacas and describes some of the problems associated with handling the viscous seminal plasma characteristic of camelids. Possibilities and limitations of reproductive biotechnologies in this species are discussed, and future developments are outlined, especially some new techniques which are currently being developed for use with camel semen and may be transferable to alpacas.

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ESTUDIO LECTINHISTOQUÍMICO DEL ÚTERO DE ALPACAS (Vicugna pacos) BAJO TRATAMIENTO SUPEROVULATORIO.

Cited by 2 publications

References 19 publications

Term alpaca placenta glycosylation profile and its correlation with pregnancy maintenance and fetal survival

Term alpaca placenta glycosylation profile and its correlation with pregnancy maintenance and fetal survival

Semen collection methods in alpacas

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