Estudo da ação da estreptoquinase e do alopurinol em retalhos cutâneos em ilha submetidos à isquemia prolongada: estudo experimental em ratos

Abstract

Objective. To establish a relation between the survival rate for island skin flaps submitted to prolonged ischemia and the effect of streptokinase and allopurinol administered following the prolonged ischemic period. MethOds. A total of 48 male Wistar rats, each weighing between 300 and 350 grams, were separated into four groups of twelve as follows: control, allopurinol, streptokinase, and association of allopurinol and streptokinase. The rats were submitted to epigastric island flap dissection, followed by epigastric vessel bundle clamping. Flaps remained in this condition for 8 hours, in normothermic global ischemia. After the ischemic period, the clamps were removed and each rat received the proposed therapeutic scheme for the group by intravenous injections. Flap survival analysis was performed on the seventh postoperative day. Variance and descriptive analyses (as percentage of necrotic area) were carried out, as well as Dunnett's T3 multiple comparisons among the four groups and the median test. Results. Rats in the control group presented an average necrosis of 79.88% in the total flap area; those receiving allopurinol presented an average necrosis of 64.05%, whereas the group receiving streptokinase presented an average necrosis of 55.52%. With the association of both drugs, rats presented an average necrosis of 54.30% in the total flap area. By applying the Dunnett's test and the median test, we were able to verify that, in this study, the streptokinase group had the lowest rate of necrosis. cOnclusiOn. Compared to the administration of allopurinol, association of allopurinol and streptokinase, and the control group, systemic administration of streptokinase after 8 hours of normothermic global ischemia resulted in an increased survival rate for epigastric island skin flaps in rats.

Efeitos Da Abordagem Fisioterapêutica Na Sintomatologia De Pacientes Em Cuidados Paliativos

“…Recentemente, a administração de estreptoquinase tem sido usado na forma de terapêutica fibrinolítica pré-hospitalar (administrada entre 30 minutos e três horas do início dos sintomas), na tentativa de reverter os processos que levam a não reperfusão, com pretensão de abreviar o tempo de isquemia miocárdica aguda e necrose muscular para, então, reduzir o tamanho do infarto do miocárdio, o que resulta na redução da mortalidade pré-hospitalar e intra-hospitalar e na redução de suas complicações imediatas e tardias, como a disfunção ventricular, o AVC e o reinfarto (MOURA et al, 2009;PIEGAS et al, 2015).…”