Estudo da atividade antibacteriana dos monoterpenos timol e carvacrol contra cepas de Escherichia coli produtoras de β-lactamases de amplo espectro

Lima, Dijaci Santos de; Lima, Jade Cardôso; Calvacanti, Raqueline Maiara Costa Bezerra; Santos, Bernadete Helena Cavalcanti dos; Lima, Igara Oliveira

doi:10.5123/s2176-62232017000100003

Cited by 16 publications

(12 citation statements)

References 19 publications

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“…In vitro studies have demonstrated the antimicrobial action of carvacrol against V. parahaemolyticus, S. aureus and Pseudomonas fluorescens which presented a MIC of 0.5, 0.125 and 0.5 mg mL -1 , respectively (Fang et al, 2019). In another study, carvacrol was evaluated against other microorganisms, such as strains of E. coli, showing a MIC of 0.064 mg mL -1 , and S. typhimurium, showing a MIC of carvacrol of 0.12 mg mL -1 (Lemos et al, 2017;Lima et al, 2017). In addition, thyme essential oil (T. vulgaris), containing 60% carvacrol, was evaluated against V. vulnificus, Vibrio fluvialis and V. parahaemolyticus with a MIC of carvacrol of 0.156 mg mL -1 ,…”

Section: Discussionmentioning

confidence: 99%

In Vitro Antimicrobial Activity of Carvacrol Against Shrimp Pathogens and Its Use as Feed Additive for the Pacific White Shrimp

et al. 2021

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This study aimed to evaluate the in vitro effect of carvacrol on different microorganisms of importance in shrimp farming, as well as its in vivo effect on zootechnical, immunological and microbiological performance, as well as resistance, of Litopenaeus vannamei challenged with Vibrio parahaemolyticus. In particular, the antimicrobial activity of carvacrol was evaluated in vitro by analysis of the minimum inhibitory concentration (MIC) and by agar diffusion disc with Gram-negative and Gram-positive bacteria. The in vivo experiment was conducted using different concentrations of carvacrol (1, 3, 4 and 6 mg mL-1) added to shrimp feed, together with a control diet without carvacrol. After four weeks, zootechnical, immunological and microbiological parameters, as well as resistance, of animals challenged with V. parahaemolyticus were evaluated. The MIC of Vibrio alginolyticus and Vibrio harveyi was 0.078 mg mL-1, while for the other bacteria, it was 0.156 mg mL-1 of carvacrol. The greatest halos of inhibition were observed in V. parahaemolyticus and Vibrio harveyi with significant differences demonstrated for the other microorganisms, except Escherichia coli. The in vivo results showed no significant differences among treatments. In conclusion, the antimicrobial activity of carvacrol was confirmed with Gram-negative and Gram-positive bacteria, and it is suggested that its antimicrobial potential is more effective against Vibrio spp. However, the concentrations of carvacrol used in vivo did not affect the parameters evaluated.

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Section: Discussionmentioning

confidence: 99%

In Vitro Antimicrobial Activity of Carvacrol Against Shrimp Pathogens and Its Use as Feed Additive for the Pacific White Shrimp

et al. 2021

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“…O. Berg, followed by compounds classified as organic acids and alcohols [ 12 ]. It is known that the essential jabuticaba oil contains terpenes, such as α-pinene, β-pinene, 1,8-cineole, α-terpineol, and β-caryophyllene, known for their antibacterial and anti-inflammatory actions, suggesting that the same volatile compounds present in the fruit aroma are indicative of endogenous levels and potential bioactive action [ 13 , 14 , 15 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Volatile Compounds and Physicochemical Quality of Four Jabuticabas (Plinia sp.)

et al. 2020

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The jabuticaba is a native Brazilian fruit that has aroused worldwide interest in terms of its nutritional composition and biological activity. However, research on the profile of volatile compounds (VOCs) emitted by these fruits is rare. This study presents the first identification of VOCs from four jabuticaba species. The aim of the study was to characterize the aromatic profile of the following species: ‘Sabará’ (Plinia jaboticaba), ‘Escarlate’ (Plinia phitrantha × Plinia cauliflora), ‘Otto Andersen’ (Plinia cauliflora), and ‘Esalq’ (Plinia phitrantha). The analysis was performed by headspace solid-phase microextraction combined with gas chromatography/mass spectrometry (SPME-GC-MS). Multivariate analysis techniques applying the partial least squares-discriminant analysis (PLS-DA) and heatmap were used to compare the results. Fruit quality parameters were determined in terms of fresh mass (g), skin color, soluble solids, and titratable acidity. A total of 117 VOCs was identified including terpenoids, esters, alcohols, aldehydes, alkanes, ketones, and carboxylic acids, with 36 VOCs common to all four species. Terpenes were the majority for all jabuticabas with smaller contributions from other volatile classes, especially β-cubebene, β-elemene, and D-limonene for the ‘Otto Andersen’ jabuticaba.

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“…As beta-lactamases são enzimas capazes de hidrolisar o anel beta-lactâmico (Figura 2a) de antibióticos como, penicilinas, cefalosporinas, carbepenêmicos e monobactâmicos. Estas enzimas são codificadas nos plasmídeos, transpósons e íntegrons, sendo elementos capazes de carregar o gene de resistência, transmitindo horizontalmente para outras bactérias (Lima, Lima, Calvacanti, Santos & Lima, 2017).…”

Section: Metodologiaunclassified

Estudo da enzima beta-lactamase e sua relação com a resistência aos antibióticos

Araújo

Azevedo

2020

RSD

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Beta-lactam antibiotics have the same mechanism of action, the inhibition of bacterial cell wall synthesis, mainly affecting peptidoglycan. They are considered safety antibiotics, since, when they directly reach the cell wall, they cause bacterial autolysis. The objective of this research is to evaluate the affinity between the beta-lactamase enzyme and several classes of beta-lactam antibiotics. This study is a research of exploratory type methodology, of quantitative nature with application of computer simulation with application of methods with computer simulation with the evaluation of the connection scores by traditional molecular anchoring using the AutoDock 4.2 program. We identified that, in the results of molecular docking simulations, penicillin and cephalosporin showed greater affinity at the catalytic site, due to a lower Gibbs free energy. It is concluded that the affinity of the antibiotics penicillin and cephalosporin may in the future represent an obstacle for the treatment of diseases such as, mild and moderate infections of the respiratory tract, skin infections, venereal infections, and the prophylaxis of rheumatic diseases. We suggest that further studies evaluate the simulations with molecular dynamics tests to see if, considering the flexibility of the enzyme, even more accurate results would be possible.

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Estudo da atividade antibacteriana dos monoterpenos timol e carvacrol contra cepas de Escherichia coli produtoras de β-lactamases de amplo espectro

Cited by 16 publications

References 19 publications

In Vitro Antimicrobial Activity of Carvacrol Against Shrimp Pathogens and Its Use as Feed Additive for the Pacific White Shrimp

In Vitro Antimicrobial Activity of Carvacrol Against Shrimp Pathogens and Its Use as Feed Additive for the Pacific White Shrimp

Volatile Compounds and Physicochemical Quality of Four Jabuticabas (Plinia sp.)

Estudo da enzima beta-lactamase e sua relação com a resistência aos antibióticos

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