Estudo da biodegradação bacteriana do herbicida glifosato por microbiota de corpo hídrico e por coleção laboratorial e análise da presença de glifosato em corpo hídrico do município de Guaratinguetá/SP

Cruvinel, Gabriela Torres

doi:10.11606/t.97.2019.tde-21112019-161425

Cited by 2 publications

(2 citation statements)

References 51 publications

(113 reference statements)

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“…ΔrelA strains [54], which suggests that glyphosate-induced growth inhibition is mostly caused by aromatic amino acid starvation, which is sufficient to halt bacterial growth, even in the absence of (p)ppGpp accumulation. Moreover, it has been argued that there is no specific molecular mechanism involved in persister formation and that any environmental stress that reduces bacterial growth would contribute to the formation of persistence [55].…”

Section: Discussionmentioning

confidence: 99%

“…These levels are considerably below glyphosate minimal inhibitory concentration and should not cause a significant change in microbial tolerance/persistence. Indeed, concentrations below 1 mM (0.17 mg/L) do not affect E. coli growth rate [54] and are unlikely to drive the bacteria into a state of dormancy with the consequent emergence of tolerance. Nevertheless, whether the concentration of glyphosate in natural environments is high enough to increase tolerance or not, our results clearly showed that glyphosate does not increase the MIC of three different antibiotics, but it does play a role on bacterial tolerance or persistence.…”

Section: Discussionmentioning

confidence: 99%

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Glyphosate affects persistence and tolerance but not antibiotic resistance

Ospino

Spira

2022

Preprint

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Glyphosate is a herbicide widely used in food production that blocks the synthesis of aromatic amino acids in plants and in microorganisms and also induces the accumulation of the alarmone (p)ppGpp. Resistance, tolerance and persistence to antibiotics are common phenomena that contribute to antibiotic treatment failure. There are several mechanisms through which bacteria attain tolerance or persistence to antibiotics, but all of them involve partial or complete growth inhibition. In this study we evaluated the effect of glyphosate on the resistance, tolerance, and persistence towards the antibiotics ampicillin, ciprofloxacin and kanamycin and the possible role of (p)ppGpp in this activity. Glyphosate did not affect the minimum inhibitory concentration of these antibiotics, but enhanced bacterial tolerance and/or persistence towards them. The upshift in ciprofloxacin and kanamycin tolerance was partially dependent on the presence of relA that promotes (p)ppGpp accumulation in response to glyphosate. Conversely, the strong increase in ampicillin tolerance caused by glyphosate was independent of relA. We conclude that by inducing aromatic amino acid starvation glyphosate contributes to the temporary increase in E. coli tolerance or persistence, but does not affect antibiotic resistance.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Glyphosate affects persistence and tolerance but not antibiotic resistance

Ospino

Spira

2022

Preprint

View full text Add to dashboard Cite

show abstract

Glyphosate affects persistence and tolerance but not antibiotic resistance

Ospino

Spira

2023

BMC Microbiol

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Glyphosate is a herbicide widely used in food production that blocks the synthesis of aromatic amino acids in plants and in microorganisms and also induces the accumulation of the alarmone (p)ppGpp. The purpose of this study was to investigate whether glyphosate affects the resistance, tolerance or persistence of bacteria towards three different classes of antibiotics and the possible role of (p)ppGpp in this activity. Glyphosate did not affect the minimum inhibitory concentration of the tested antibiotics, but enhanced bacterial tolerance and/or persistence towards them. The upshift in ciprofloxacin and kanamycin tolerance was partially dependent on the presence of relA that promotes (p)ppGpp accumulation in response to glyphosate. Conversely, the strong increase in ampicillin tolerance caused by glyphosate was independent of relA. We conclude that by inducing aromatic amino acid starvation glyphosate contributes to the temporary increase in E. coli tolerance or persistence, but does not affect antibiotic resistance.

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Estudo da biodegradação bacteriana do herbicida glifosato por microbiota de corpo hídrico e por coleção laboratorial e análise da presença de glifosato em corpo hídrico do município de Guaratinguetá/SP

Cited by 2 publications

References 51 publications

Glyphosate affects persistence and tolerance but not antibiotic resistance

Glyphosate affects persistence and tolerance but not antibiotic resistance

Glyphosate affects persistence and tolerance but not antibiotic resistance

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