This study aimed at evaluating the practices adopted on the management of Healthcare Waste (HW) and how the ANVISA resolution-RDC nº 306/04 was applied on procedures at a large hospital. For that, it was taken as case study the hospital of Irmandade Santa Casa de Misericórdia de São Carlos, because they already have a historic of research production, which served as the basis for a comparative study of composition of medical waste generated, besides the temporal evolution of implemented procedures for local management. The method to evaluate the waste management was done through exploratory and documentary research. On the research development, semi-structured interviews were made and questionnaires applied to hospital staff for evaluation of their own involvement in waste management. The hospital sectors were classified as critical, semi-critical and non-critical areas for evaluation of risks involved. The generating sources, routine and key-procedures of waste management were identified in each mapped area. As part of results, the staff capacitating promoted a decrease in volume in group A waste and in work accidents by puncture-cutting instruments. The hospital had an initial waste generation rate of e.09 kg/patient/day, constituted by groups A (21.7%), D (76.1%) and E (2.2%). The group D waste was characterized on administrative sectors, where there was a predominance of paper and cardboard, indicating a recycling potential. The volume of organic waste generated in the kitchen justifies the search of alternatives for treatment, such as composting. The results indicate that there was a partial incorporation of legislation principles on the implanted model, showing significant results on waste segregation step. The critical points identified are mainly related to the need for adequacy of hospital physical structure, systematic staff training and education campaigns with establishment users, not only for comply of the legal demands, but also to maintain the collective, occupational and environmental health.