Estudo da microbiota fúngica da conjuntiva ocular em portadores de hanseníase e seus comunicantes

Santos, Procópio Miguel dos; Melo, Cinthia Mendonça de; Martins, Suy Anne Rebouças; Chaves, Adriano de Almeida; Sá, Débora Silva Pires de; Santos, Rosa Maria Nascimento dos

doi:10.1590/s0004-27492006000600022

Cited by 5 publications

(9 citation statements)

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“…Located in different regions of Brazil, Sorocaba has a population of 500,000 and large economic diversity (16) , in contrast to the city of Rio Branco, which has a population of approximately 350,000 and a barely sustainable economy based on agricultural activities (15) . Despite the fact that the literature shows the possible influence of climate and socioeconomic conditions on conjunctival microbiota (bacterial and fungal) (4,10,(12)(13)(14) , no significant differences were observed in the regions studied with regard to bacterial growth.…”

Section: Discussionmentioning

confidence: 67%

“…Although the literature also demonstrates the presence of anaerobic bacteria and fungi, the object of the current study was to evaluate the aerobic bacteria in this context (13,14) . The main aim of the current study was to study aerobic bacterial microbiota in the conjunctiva of diabetic patients in terms of the level of control of diabetes, assessed using glycated hemoglobin levels.…”

Section: Resultados: Foram Incluídos 120 Olhos De 120 Pacientes a Pomentioning

confidence: 99%

“…The changes found in the microbiota may depend on social and personal factors, on the predominant geographical area, as well as on the resistance that may be present against the empirically administered antibiotics (23) . The profile of the ocular microbiota undergoes constant change, influenced by seasonal variations, temperature, age, environmental exposure factors, surgical trauma and host immunity (4,10,(12)(13)(14) . The characteristics of the two cities studied are, respectively, an equatorial climate with hot and rainy weather in Rio Branco (15) , and a subtropical and temperate climate in Sorocaba (16) .…”

Section: Discussionmentioning

confidence: 99%

“…S. epidermidis, S. aureus, and Corynebacterium sp have also been found in diabetic patients (4) . Variations in conjunctival microbiota may occur due to both intrinsic and extrinsic changes in the body, such as geographical diversity and different socioeconomic characteristics (4,10,(12)(13)(14) . Therefore, the conjunctival aerobic microbiota of diabetic patients was studied in two different Brazilian cities: Rio Branco, in the state of Acre, in north Brazil; and Sorocaba, in the state of São Paulo, in southeastern Brazil.…”

Section: Introductionmentioning

confidence: 99%

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Aerobic bacterial microbiota of the conjunctiva in diabetic patients with normal and altered glycated hemoglobin levels in two regions in Brazil

Moreno¹,

Moreno²,

Sousa³

2014

Arquivos Brasileiros De Oftalmologia

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Purpose: To study the aerobic bacterial microbiota of the conjunctiva in diabetic patients with regard to the management of diabetes, assessed using glycated hemoglobin levels. Methods: A cross-sectional study was conducted using conjunctival smears of diabetic patients from both sexes and with different ages, residing in two different Brazilian cities (Sorocaba and Rio Branco). A control group of non-diabetic patients was also included. The diabetic patients were considered to have controlled diabetes when their glycated hemoglobin level was ≤7% and blood glucose level was ≤126 mg/dL. Patients with non-controlled diabetes were those with glycated hemoglobin levels >7% and blood glucose levels >126 mg/dL. The samples obtained were inoculated in Brain-Heart Infusion broth and in culture media for aerobic bacteria (blood and chocolate agars); bacterial growth was evaluated in a microbiology laboratory. Results: A total of 120 eyes of 120 patients were included in the present study. The percentage of cultures in which bacterial growth was observed was greater in diabetic patients, although the difference was not statistically significant (p=0.103). There was a greater trend toward bacterial growth in the conjunctiva of diabetic patients with altered fasting blood glucose. There was no difference in the frequency of bacterial growth on the conjunctiva between diabetic patients with normal or altered glycated hemoglobin levels. In Sorocaba, conjunctival bacterial growth was similar to that observed in Rio Branco. The microorganism most frequently detected in the present study was Staphylococcus epidermidis, followed by Staphylococcus aureus, Proteus mirabilis, and Escherichia coli. Conclusions: There was no difference between diabetic patients with normal or altered glycated hemoglobin levels. The microorganisms found were similar to those found in studies investigating the conjunctival bacterial flora of diabetic and non-diabetic patients.

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Section: Discussionmentioning

confidence: 67%

Section: Resultados: Foram Incluídos 120 Olhos De 120 Pacientes a Pomentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Aerobic bacterial microbiota of the conjunctiva in diabetic patients with normal and altered glycated hemoglobin levels in two regions in Brazil

Moreno¹,

Moreno²,

Sousa³

2014

Arquivos Brasileiros De Oftalmologia

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“…By studying the flora of the conjunctiva, the preventive and epidemiological aspects of the disease are highlighted, which is important now that the frequency of opportunistic infections is increasing. Due to this, the focus is more directed to bacteriology (5)(6)(7)(8)(9)(10)(11)(12) , and the presence of saprophytic fungi in the conjunctiva is stressed (13)(14)(15)(16) . Some articles focus on fungal keratitis (17,18) .…”

Section: Discussionmentioning

confidence: 99%

Reflexões sobre a ceratite fúngica por meio dos achados de exames histopatológicos

Monte

Stadtherr

2013

Rev. bras.oftalmol.

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Purpose: The study of fungal invasion and pathogenicity in corneal tissue observed through the histopathological examination of specimens obtained through penetrating keratoplasty ('PKP') of samples obtained from an Eye Bank ('EB'), with the aim of applying findings in diagnosis and treatment of the condition (n=35), a faixa etária acima de 60 anos a mais numerosa com 1/3 dos pacientes. Os casos de ceratite fúngica correspondiam à média de 6,4% (n=597) do material recebido no BOO e 1,65% (n=2310) dos transplantes ocorrido com o material fornecido nos últimos 5 anos. Pelas informações dos cirurgiões 39,5% (n=38) dos casos deviam-se a perfuração corneana. Usando as datas dos transplantes foi feita uma Tábua de Observação. Em 11 (n=38) casos, a córnea procedia de transplante anterior. As formas leveduriformes nos tecidos corneanos eram de 63% (n=38). Em 50% (n=38) dos casos o infiltrado inflamatório era pequeno ou inexistente. A camada de Descemet estava íntegra em 13% (n=38), enquanto eram encontrados fungos na superfície corneana de 45% (n=38) dos casos. Conclusão: A coleta do material poderá ser feita com sucesso mesmo depois de instalado o tratamento, entretanto, nas úlceras de córnea deve ser feito preferentemente a coleta de material com espátula para exame laboratorial e a microscopia confocal in vivo. A predominância das leveduras poderá ser devido a alterações morfológicas do fungo sofridas no tecido corneano. A penetração intraocular é facilitada por alterações da Camada de Descemet e pela própria capacidade do fungo de penetrar nos tecidos justificando o tratamento sistêmico desde o início. Descritores: Ceratite/diagnóstico; Ceratite/patologia; Infecções oculares fúngicas/diagnósticoThe authors declare no conflicts of interest

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The human mycobiome in health and disease

2013

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The mycobiome, referring primarily to the fungal biota in an environment, is an important component of the human microbiome. Despite its importance, it has remained understudied. New culture-independent approaches to determine microbial diversity, such as next-generation sequencing methods, are greatly broadening our view of fungal importance. An integrative analysis of current studies shows that different body sites harbor specific fungal populations, and that diverse mycobiome patterns are associated with various diseases. By interfacing with other biomes, as well as with the host, the mycobiome probably contributes to the progression of fungus-associated diseases and plays an important role in health and disease.

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Estudo da microbiota fúngica da conjuntiva ocular em portadores de hanseníase e seus comunicantes

Abstract: The most frequent yeast isolated from conjunctival samples of leprosy patients was Candida, which could be explained by cellular immunodeficiency of these patients, associated with dry eye and the prolonged use of antibiotics.

Cited by 5 publications

References 9 publications

Aerobic bacterial microbiota of the conjunctiva in diabetic patients with normal and altered glycated hemoglobin levels in two regions in Brazil

Aerobic bacterial microbiota of the conjunctiva in diabetic patients with normal and altered glycated hemoglobin levels in two regions in Brazil

Reflexões sobre a ceratite fúngica por meio dos achados de exames histopatológicos

The human mycobiome in health and disease

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