Estudo Da Resistência Aos Antifúngicos De Leveduras Isoladas De Candidúrias De Um Hospital De Médio Porte

Moreira, Laís Santos; Doria, Anelise C.O.C.; Santos, Thaisa Baesso; Figueira, Fernanda Ramos; Sorge, Camila Di Paula Costa; Silva, Adriano Moraes da; Khouri, Sônia

doi:10.18066/revistaunivap.v23i43.365

Cited by 5 publications

(4 citation statements)

References 6 publications

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“…However, there have been reports of Candida spp. resistance to antifungals commonly used in clinical practice [29].…”

Section: Resultsmentioning

confidence: 99%

Antifungal and Antibiofilm Activities of Ozonated Sunflower Oil against Candida albicans Strains: A Pilot Study and Clinical Perspectives

Araújo,

Prado,

Aguiar

et al. 2024

Arch. Curr. Res. Int.

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Aims: This study aimed to assess the antifungal and antibiofilm activities of ozonized (OGOZ) and non-ozonized (OG) Helianthus annuus (sunflower) oil against Candida albicans strains. Study Design: Laboratory-based study. Place and Duration of Study: Microbiology Laboratory of the Federal University of Ceará and State University of Vale do Acaraú, from August 2023 to Decembrer of the same year. Methodology: Seven clinical strains and one reference strain (ATCC 90028) were examined. Initially, antimicrobial susceptibility testing (AST) was performed using a modified disk diffusion method with both OGOZ and OG. Strains that displayed sensitivity were then subjected to a broth microdilution assay to determine the Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC). The biofilm assay was conducted specifically with the C. albicans reference strain. Results: AST results showed that all strains exhibited inhibition zones with OGOZ, while non-ozonized oil did not produce inhibition zones. MIC values ranged from 0.15 to 2.50 mg/mL, with one clinical strain (LABMIC H10) demonstrating an MIC value 16 times lower than other strains. None of the strains reached MFC at the tested concentrations. In terms of biofilm formation, application of MIC and twice the MIC resulted in a 48,6% and 85% reduction in mature biofilms, respectively. Conclusion: The findings indicate that OGOZ has fungistatic and antibiofilm effects, highlighting its potential as a promising agent for bioprospecting. Further clinical trials are essential to evaluate its therapeutic potential for managing infectious diseases such as oral candidiasis.

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“…However, there have been reports of Candida spp. resistance to antifungals commonly used in clinical practice [29].…”

Section: Resultsmentioning

confidence: 99%

Antifungal and Antibiofilm Activities of Ozonated Sunflower Oil against Candida albicans Strains: A Pilot Study and Clinical Perspectives

Araújo,

Prado,

Aguiar

et al. 2024

Arch. Curr. Res. Int.

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“…Worryingly, increased resistance to antifungal drugs threatens the effectiveness of treatments used to combat infections caused by this microorganism [ 60 ]. In this context, a study analyzing antifungal resistance in infections by Candida species, including C. albicans , in Brazilian hospitals recorded high rates of resistance to conventional antifungal agents such as fluconazole (88.89%), miconazole (66.67%), nystatin (55.56%), and amphotericin B (50.00%) [ 61 ]. Similar studies found resistance rates to fluconazole in C. albicans , ranging between 61% and 100% [ 62 , 63 ].…”

Section: Discussionmentioning

confidence: 99%

Chemical Constituents and Biological Activities of Croton heliotropiifolius Kunth

et al. 2021

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Croton heliotropiifolius Kunth (Euphorbiaceae), whose occurrence has already been registered in the most varied Brazilian biomes, is commonly found in the Chapada do Araripe, Ceará. The species is traditionally used to treat fungal, parasitic, and degenerative diseases. This study investigated the chemical composition and pharmacological potential (antioxidant, antifungal, antiparasitic, and cytotoxic) of an aqueous extract obtained from the roots of C. heliotropiifolius. Following a qualitative phytochemical screening, the chemical constituents were identified by ultra-efficiency liquid chromatography coupled witha quadrupole/time-of-flight system (UPLC-QTOF). The antioxidant potential was verified by thin-layer chromatography (TLC). The direct and combined antifungal activity of the extract against opportunistic Candida strains was investigated using the microdilution method. The minimal fungicidal concentration (MFC) was determined by subculture, while the modulation of the morphological transition (fungal virulence) was evaluated by light microscopy. The in vitro antiparasitic activity was analyzed using epimastigotes of Trypanosoma cruzi and promastigotes of Leishmania braziliensis and Leishmania infantum, while cytotoxicity was determined in cultures of mouse fibroblasts. The phytochemical analysis identified the presence of acids, terpenes, flavonoids, lignans, and alkaloids. Among these constituents, the presence of polar and non-polar phenolic compounds with known antioxidant action washighlighted. While the extract showed clinically ineffective antifungal effects, it could enhance the effectiveness of fluconazole, in addition to inhibiting the morphological transition associated with increased virulence in Candida strains. Although the extract showed low cytotoxicity against fibroblasts, it also had weak antiparasitic effects. In conclusion, Croton heliotropiifolius is a source of natural products with antifungal and antioxidant potential.

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“…Devido ao fato de que esse esterol existe apenas na célula fúngica e não nas células de mamíferos, ele se torna um alvo seletivo para os antifúngicos, buscando-se, assim, novas substâncias que atuem especialmente no ergosterol, não causando efeito adversos nas células humanas. 26,30,31 Substâncias que atuam no ergosterol da membrana tendem a ligar-se no ergosterol exógeno, que está no meio enriquecido, desviando sua atividade do ergosterol da membrana fúngica. Consequentemente, será necessária uma maior quantidade da substância testada para inibir o crescimento fúngico da mesma quantidade de inóculo.…”

Section: Discussionunclassified

Antifungal Activity of Latex and Lupenonefrom Jatropha multifida L. (Euphorbiaceae)

Oliveira¹,

Lemos²,

Pereira³

et al. 2019

Rev. Virtual Quim.

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Jatropha multifida L. belongs to the family Euphorbiaceae, being popularly used in Brazil for treatment and healing of infected wounds, gastrointestinal ulcers and candidiasis. Lupenone, isolated from the hexane extract of its leaves, was submitted to the synergism test for Candida tropicalis and evaluation of its possible mechanism of antifungal action. Lupenone was able to halve the minimum inhibitory capacity (MIC) of fluconazole against C. tropicalis with possible action on the cell wall and ergosterol of the fungal membrane. The fraction derived from successive fractions of the aqueous latex residue, was analyzed by HPLC-DAD and tested in the antifungal assay against the following yeasts: Candida albicans, Candida albicans Serology A and B, Candida grablata, Candida krusei, Candida tropicalis and Cryptococcus neoformans. This fraction showed a probable catechin with moderate activity against C. tropicalis and C. grablata. The presence of triterpene and polyphenols in J. multifida may be responsible for the antifungal and cicatrizant activities presented by the species. Lupenone may be used in combination with fluconazole in the development of a new antifungal.

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Estudo Da Resistência Aos Antifúngicos De Leveduras Isoladas De Candidúrias De Um Hospital De Médio Porte

Abstract: Resumo: Devido à alta incidência de infecções graves, tem se buscado novas terapias antifúngicas

Cited by 5 publications

References 6 publications

Antifungal and Antibiofilm Activities of Ozonated Sunflower Oil against Candida albicans Strains: A Pilot Study and Clinical Perspectives

Antifungal and Antibiofilm Activities of Ozonated Sunflower Oil against Candida albicans Strains: A Pilot Study and Clinical Perspectives

Chemical Constituents and Biological Activities of Croton heliotropiifolius Kunth

Antifungal Activity of Latex and Lupenonefrom Jatropha multifida L. (Euphorbiaceae)

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