Estudo morfológico de células sanguíneas de Piaractus brachypomus (Cuvier, 1817)

Abstract

O estudo morfológico de células sanguíneas é considerado como a base para diversas pesquisas, uma vez que a forma da célula, disposição do seu núcleo e o nível de condensação da cromatina são de fácil observação, além de detectar patologias que causam anormalidades no sangue. Assim, este estudo teve o objetivo de avaliar a morfologia das células sanguíneas encontradas na contagem qualitativa da espécie Piaractus brachypomus. Foram analisadas células de 43 peixes com peso médio de 1,34 Kg e comprimento total 39,23 cm, num total de 43000 células observadas. Assim, foi possível verificar a ocorrência de eritrócitos, trombócitos, e seis tipos de leucócitos: basófilo, eosinófilo, neutrófilo, heterófilo e monócito. Além disso, observou-se a presença de neutrófilos tóxicos, além de uma pequena quantidade de alterações morfológicas no núcleo das células como micronúcleos e invaginações. Durante o experimento, não houve morte de animais. No geral, as células sanguíneas de P. brachypomus apresentaram semelhanças morfológicas, quando comparada com a morfologia das células de peixes ósseos. Palavras chave: células sanguíneas, estudo morfológico, Piaractus brachypomus Morphological study of blood cells of Piaractus brachypomus (Cuvier, 1817)ABSTRACT. The morphological study of blood cells is considered as the basis of several studies, since the shape of the cell, its nucleus arrangement and the level of chromatin condensation are easy to note, as well as detecting pathologies that cause blood abnormalities. The aim of this study was to evaluate the morphology of blood cells found in the qualitative count of the species Piaractus brachypomus. There were analysed cells from 43 fishes, with an average weigth of 1.34 Kg and a mean length of 39.23 cm, in a total of 43000 cells were observed, thus it was possible to verify the ocurrence of erythrocyte, thrombocytes and six types of leukocytes: lymphocytes, basophils, eosinophils, neutrophils, heterophiles and monocytes. In addition, it was noticed toxic neutrophils and a small number of morphological nuclear changes such as micronuclei and invaginations In general the blood cells of P. brachypomus presented morphological similarities when compared to the morphology of the bone fish cells.

Effects of peptides and probiotics supplementation via diet on blood parameters and growth performance of Piaractus brachypomus during the growth-out phase

“…At zero (as basal sample), second, fourth, sixth, and eighth week of the trial, blood was collected from one previously anesthetized (1.50 ml/l of eugenol) fish, randomly captured per tank, via caudal vessel puncture using 10% EDTA solution as the anticoagulant ( Dos Santos et al ., 2021 ). Blood was analyzed by hematocrit (Ht – %) using heparinized microhematocrit capillary tubes (Corning ® ), centrifuged at 900 rpm (X–3,012), and analyzed with an hematocrit reader (CRIPTOCAT); by white blood cells count in 1 mm 3 (neutrophils – %, eosinophils – %, basophils – %, lymphocytes – %, and monocytes – %) according to De Oliveira et al . (2018) .…”

Growth, red blood cells, and gill alterations of red pacu (Piaractus brachypomus) fingerlings by chronic exposure to different total suspended solids in biofloc

“…Red pacu, P. brachypomus , hematological parameters in semi‐intensive production systems were reported by Garay and Paredes (2011) and Tocidlowsky, Lewbart, and Stoskopf (1997). Other hematological parameters as the morphology of red pacu blood cells (De Oliveira, Oliveira, & dos Santos Souto, 2018), blood chemistry values (Sakamoto, Lewbart, & Smith, 2001), and nonspecific immune response (Lochmann et al, 2009) have been reported.…”