Introduction:Group B Streptococcus (GBS) is a gram-negative diplococcus found in women as part of the vaginal flora. It is the main risk factor for early neonatal infection, causing up to 25% of deaths in affected newborns. Objective: To determine the prevalence of group B streptococcus colonization in pregnant women who attended the Obstetrics, Emergency Department and Delivery Room at the Lucrécia Paim and Augusto N'gangula Maternity Hospital in Luanda, from January to March 2019. Methodology: A prospective cross-sectional study was carried out on 382 pregnant women, of which 183 were excluded. Vaginal and anorectal specimens were collected using a sterile swab. The samples obtained were stored in Stuart transport medium, inoculated in Stuart selective medium with subsequent subculture on blood agar plates. Socioeconomic and clinical-obstetric variables were also analyzed. Data were analyzed using the SPSS program. The non-probabilistic sampling technique for consecutive convenience was used. Results: The prevalence of maternal GBS colonization was 38.5%. There was a higher prevalence of GBS in the age group of 21 -30 years and in multiparous women, with 46% and 42.8% of the cases, respectively. Gestational age greater than 35 weeks was the risk factor with the greatest influence on GBS colonization and in the comparative analysis it showed a statistically significant association (p=0.02).Conclusion: The prevalence of group B streptococcus in our country is high. Screening is essential for all pregnant women for diagnosis, treatment, reduction of vertical transmission and reduction of cases of neonatal infection.