ETAS, an Enzyme-treated Asparagus Extract, Attenuates Amyloid β-Induced Cellular Disorder in PC 12 Cells

Ogasawara, Jun; Ito, Tomohiro; Wakame, Koji; Kitadate, Kentaro; Sakurai, Takeshi; Sato, Shogo; Ishibashi, Yoshinaga; Izawa, Tetsuya; Takahashi, Kazuto; Ishida, Hideyuki; Takabatake, Ichiro; Kizaki, Takako; Ohno, Hideki

doi:10.1177/1934578x1400900435

Cited by 11 publications

(14 citation statements)

References 35 publications

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“…A recent study also showed that ETAS 50 supplementation reduced the feelings of dysphoria and fatigue, ameliorated the quality of sleep, and enhanced stress-load performance as well as increasing salivary secretory immunoglobulin A levels in healthy adults [ 16 ]. In addition to these anti-stress effects, ETAS 50 has antiaging potencies via its ability to ameliorate cognitive impairment in senescence-accelerated mice [ 17 ], to protect neuronal PC-12 cells from amyloid β peptide-induced oxidative stress and cytotoxicity [ 18 ], and to improve learning ability in young healthy rats [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Anti‐Inflammatory Effect of ETAS®50 by Inhibiting Nuclear Factor‐κB p65 Nuclear Import in Ultraviolet‐B‐Irradiated Normal Human Dermal Fibroblasts

Shirato

Koda

Takanari

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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Ultraviolet (UV) irradiation induces proinflammatory responses in skin cells, including dermal fibroblasts, accelerating premature skin aging (photoaging). ETAS 50, a standardized extract from the Asparagus officinalis stem, is a novel and unique functional food that suppresses proinflammatory responses of hydrogen peroxide-stimulated skin fibroblasts and interleukin- (IL-) 1β-stimulated hepatocytes. To elucidate its antiphotoaging potencies, we examined whether ETAS 50 treatment after UV-B irradiation attenuates proinflammatory responses of normal human dermal fibroblasts (NHDFs). UV-B-irradiated NHDFs showed reduced levels of the cytosolic inhibitor of nuclear factor-κB α (IκBα) protein and increased levels of nuclear p65 protein. The nuclear factor-κB nuclear translocation inhibitor JSH-23 abolished UV-B irradiation-induced IL-1β mRNA expression, indicating that p65 regulates transcriptional induction. ETAS 50 also markedly suppressed UV-B irradiation-induced increases in IL-1β mRNA levels. Immunofluorescence analysis revealed that ETAS 50 retained p65 in the cytosol after UV-B irradiation. Western blotting also showed that ETAS 50 suppressed the UV-B irradiation-induced increases in nuclear p65 protein. Moreover, ETAS 50 clearly suppressed UV-B irradiation-induced distribution of importin-α protein levels in the nucleus without recovering cytosolic IκBα protein levels. These results suggest that ETAS 50 exerts anti-inflammatory effects on UV-B-irradiated NHDFs by suppressing the nuclear import machinery of p65. Therefore, ETAS 50 may prevent photoaging by suppressing UV irradiation-induced proinflammatory responses of dermal fibroblasts.

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Section: Introductionmentioning

confidence: 99%

Anti‐Inflammatory Effect of ETAS®50 by Inhibiting Nuclear Factor‐κB p65 Nuclear Import in Ultraviolet‐B‐Irradiated Normal Human Dermal Fibroblasts

Shirato

Koda

Takanari

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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“…It is a unique eco-friendly functional food that attenuates sleep deprivation-induced stress responses, promotes good sleep, and increases salivary secretory immunoglobulin A levels in mice and humans [ 13 – 15 ]. In addition to its antistress effects, later studies demonstrated the beneficial effects of ETAS 50 on brain functions, including the ability to ameliorate cognitive impairment in senescence-accelerated mice [ 16 ], protect neuronal PC-12 cells from amyloid β -induced cytotoxicity [ 17 ], and improve learning ability in young healthy rats [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

ETAS®50 Attenuates Ultraviolet‐B‐Induced Interleukin‐6 Expression by Suppressing Akt Phosphorylation in Normal Human Dermal Fibroblasts

Shirato

Koda

Takanari

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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We recently reported that ETAS 50, a standardized extract from the Asparagus officinalis stem, exerted anti-inflammatory effects on ultraviolet-B- (UV-B-) irradiated normal human dermal fibroblasts (NHDFs) by inhibiting nuclear factor-κB p65 nuclear import and the resulting interleukin-1β (IL-1β) expression. To further elucidate the antiphotoaging potency of ETAS 50, we examined the anti-inflammatory effects on UV-B-irradiated NHDFs by focusing on the stress-activated mitogen-activated protein kinase (MAPK) and Akt signaling pathways. NHDFs were treated with 1 mg/mL of ETAS 50 or dextrin (vehicle control) after UV-B irradiation (20 mJ/cm2) for different time periods. Phosphorylation levels of c-Jun N-terminal kinase (JNK), p38 MAPK, and Akt were analyzed by western blotting. IL-6 mRNA levels were analyzed by real-time polymerase chain reaction. UV-B-irradiated NHDFs showed increased phosphorylation levels of JNK, p38 MAPK, and Akt, as well as increased mRNA levels of IL-6. ETAS 50 treatment after UV-B irradiation suppressed the increased phosphorylation levels of Akt without affecting those of JNK and p38 MAPK. ETAS 50 as well as Akt inhibitor Perifosine repressed UV-B irradiation-induced IL-6 mRNA expression. These results suggest that ETAS 50 treatment represses UV-B irradiation-induced IL-6 expression by suppressing Akt phosphorylation. The present findings demonstrate the potential of ETAS 50 to prevent photoaging by attenuating UV-B irradiation-induced proinflammatory responses in skin fibroblasts.

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“…Neuroprotection due to antioxidative effects could be another mechanism contributing to results observed in this clinical trial. In an in vitro cell study by Ogasawara et al 2014, asparagus extract was shown to scavenge the negative effect of free radicals at the cellular level [53]. In another study, asparagus extract was demonstrated to enhance expression of cytoprotective factors such as heat shock transcription factor 1 (HSF1) and Nrf2 protein [54].…”

Section: Discussionmentioning

confidence: 99%

Sleep Promoting Effects of IQP-AO-101: A Double-Blind, Randomized, Placebo-Controlled Exploratory Trial

Bongartz

Tan²,

Seibt

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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Objective. The purpose of this study was to explore the clinical benefit and tolerability of IQP-AO-101 in healthy subjects with sleep complaints. Methods. This double-blind, randomized, placebo-controlled trial involved fifty subjects with sleep complaints. Subjects with a Pittsburgh Sleep Quality Index (PSQI) score between 6 and 15 were randomized to receive either IQP-AO-101 or placebo for 6 weeks, following a run-in period of one week. Sleep parameters were assessed at baseline and after 1, 4, and 6 weeks using the modified Athens Insomnia Scale (mAIS). Subjects were also instructed to wear an activity tracker and keep a sleep diary during the study. Other questionnaires administered were the Frankfurt Attention Inventory (FAIR-2) and the Profile of Mood States (POMS-65). Blood samples for safety laboratory parameters were taken before and at the end of the study. Results. After 6 weeks, subjects who consumed IQP-AO-101 reported significant improvements in mAIS scores compared with placebo, including mAIS total score (11.76 ± 6.85 vs 4.00 ± 4.80; p < 0.001); night parameters composite score (5.20 ± 3.80 vs 2.04 ± 3.16; p = 0.001); and day parameters composite score (6.56 ± 4.10 vs 1.96 ± 2.65; p < 0.001). All individual parameters (Items 1 to 8) were also significantly improved from baseline after 6 weeks of IQP-AO-101 intake. Analysis of variance with baseline values as covariates showed statistically significant improvements across all individual parameters for IQP-AO-101 when compared to placebo. The measurements using the activity tracker, sleep diary, FAIR-2, and POMS did not reveal any significant differences between groups. No adverse effects related to the intake of IQP-AO-101 were reported. Tolerability was rated as very good by all the subjects and by the investigator for all cases. Conclusions. In this study, IQP-AO-101 was well tolerated and efficacious for promoting sleep and enhancing daytime performance in subjects with moderate sleep disturbances. Clinical Trial Registration. This trial is registered with ClinicalTrials.gov, no. NCT03114696.

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ETAS, an Enzyme-treated Asparagus Extract, Attenuates Amyloid β-Induced Cellular Disorder in PC 12 Cells

Cited by 11 publications

References 35 publications

Anti‐Inflammatory Effect of ETAS®50 by Inhibiting Nuclear Factor‐κB p65 Nuclear Import in Ultraviolet‐B‐Irradiated Normal Human Dermal Fibroblasts

Anti‐Inflammatory Effect of ETAS®50 by Inhibiting Nuclear Factor‐κB p65 Nuclear Import in Ultraviolet‐B‐Irradiated Normal Human Dermal Fibroblasts

ETAS®50 Attenuates Ultraviolet‐B‐Induced Interleukin‐6 Expression by Suppressing Akt Phosphorylation in Normal Human Dermal Fibroblasts

Sleep Promoting Effects of IQP-AO-101: A Double-Blind, Randomized, Placebo-Controlled Exploratory Trial

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