Ethanol analysis from biological samples by dual rail robotic autosampler

Morris-Kukoski, Cynthia L.; Jagerdeo, Eshwar; Schaff, Jason E.; LeBeau, Marc A.

doi:10.1016/j.jchromb.2006.11.034

Cited by 13 publications

(2 citation statements)

References 22 publications

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“…It allows a relatively large number of samples to be analyzed quickly, with a minimal amount of manual handling. Due to the complex matrix of biological samples, static headspace sampling is the sampling method used for ethanol analysis because the column and injector are protected so that contamination will not occur; while GC/FID is employed for analyte separation and detection [11,17,18].…”

Section: Introductionmentioning

confidence: 99%

Validation of a method for ethanol analysis in biological and non-biological samples and its toxicological application

Kostić

Vujović

Milosavljevic

2021

Hem Ind

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A simple, cost-effective and fast headspace gas chromatography method coupled with flame ionization detection (HS-GC/FID) for determination of ethanol was developed and validated for clinical and forensic toxicology purposes. HS-GC/FID is often used for alcohol determination in different biological and non-biological samples. The calibration dependence of the method was linear in the range from 0.15 to 4.00 g dm-3 (r2=0.999) with adequate accuracy (99-106 %) and precision. The limit of detection (LOD) was 0.006 g dm-3. The method was quantitative (LOQ) above 0.020 g dm-3. The new method was successfully used for determination of ethanol in biological samples of intoxicated patients, car accidents participants, participants in criminal acts, and postmortem samples, non-biological samples such as alcoholic beverages, alcohol-based herbal preparations, cosmetic preparations, etc. This method is easy to perform, making it suitable not only for the routine applications in clinical biochemistry and forensic laboratories, but also in different fields of industry (e.g. for pharmaceutical preparations, cosmetics, dietary supplements, etc.). Some of the applications for ethanol determination in different samples related to various clinical-forensic cases are presented.

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Section: Introductionmentioning

confidence: 99%

Validation of a method for ethanol analysis in biological and non-biological samples and its toxicological application

Kostić

Vujović

Milosavljevic

2021

Hem Ind

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“…It should be pointed out that ethanol in alcoholic beverages has already been determined and quantified by GC-MS. [15][16][17] All the cited examples involved sample dilution and ethanol determination using the calibration curve technique or the addition of expensive deuterated ethanol. Ethanol was also determined either as a component or an impurity, eg, in biological samples, 18 perfumery products, 19 and solid meals. 20 In order not to saturate the detector with ethanol, some authors diluted the sample with water to decrease its content 21 or interrupted the measurement during the period whilst the ethanol peak appeared in the chromatogram.…”

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confidence: 99%

The perspectives of ethanol usage as an internal standard for the quantification of volatile compounds in alcoholic products by GC‐MS

et al. 2020

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The potential use of ethanol as an internal standard (IS) for GC‐MS analysis was studied. The paper describes the analysis of spirit drinks and other alcoholic products which consist of a mixture of water, ethanol, and volatile compounds. In the suggested method, ethanol was employed as an IS for the common procedure of volatile compounds quantification. A number of standard solutions of nine compounds with different concentrations was prepared in a water‐ethanol matrix and measured with GC‐MS in the SIM mode. Two possible approaches were suggested to avoid detector saturation during ethanol detection. The first one consisted in using less abundant m/z 47 as quantifiers. These ions mainly correspond to unfragmented heavy ethanol molecules containing one 13C isotope. The second method consisted in reduction of the voltage of MS electron multiplier. The experiment also included the preparation and subsequent dilution of the standard solution and ethanol with water, which determined the linearity of the modified MS response relative to the ethanol content. Analysis of the obtained results revealed that volatile compounds can be successfully accurately determined with GC‐MS by employing ethanol as an IS. Application of the suggested method is not limited to the reported volatile compounds and alcoholic products.

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Sample Preparation Automation for GC Injection

Lord

Pfannkoch²

2012

Comprehensive Sampling and Sample Preparation

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Ethanol analysis from biological samples by dual rail robotic autosampler

Cited by 13 publications

References 22 publications

Validation of a method for ethanol analysis in biological and non-biological samples and its toxicological application

Validation of a method for ethanol analysis in biological and non-biological samples and its toxicological application

The perspectives of ethanol usage as an internal standard for the quantification of volatile compounds in alcoholic products by GC‐MS

Sample Preparation Automation for GC Injection

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