Ethanol and Isopropanol detoxification associated with the Adh locus of Drosophila melanogaster

Abstract

The detoxification of both ethanol and isopropanol in relation to the Adh locus was studied in the "Lagar de los Reyes" (LR) lines of Drosophila melanogaster. Homozygous lines for the Adh' and the Adhs alleles were kept on an ethanol-supplemented medium for about 60 generations. After the selection, adult flies from these selected lines (LRSeF and LRSeS) were tested for utilisation of, and tolerance to, ethanol and isopropanol, and compared with appropriate controls (LRCF and LRCS) kept on regular medium. Both LRC and LRSe were able to use ethanol (but not isopropanol) as food, the selected flies showing a larger utilisation range than controls. Independently of the lines, FF flies showed a higher or equal ethanol and isopropanol tolerance than SS animals, the females being more or equally tolerant compared with the males. Increased tolerance to ethanol (but not to isopropanol detoxification is equally tolerant compared with the males. Increased tolerance to ethanol (but not to isopropanol) was found in the selected lines. Because of this, a partial independence of ethanol and isoproponal detoxification is suggested.

Ingestible insecticides for spotted wing Drosophila control: a polyol, Erythritol, and an insect growth regulator, Lufenuron

“…Dead adults resemble upright shrivelled mummies; hinting at a possible mode of action for Erythritol, osmotic shock followed by cellular desiccation (Sánchez‐Cañete et al. ). In fact, when relative humidity drops below 50%, increased water loss in adult SWD becomes fatal (Tochen et al.…”

Differences in the Effect of Ethanol on Fertility and Viability Components Among Laboratory Strains of Drosophila Melanogaster

“…At the same time, the influence of the Adh locus was far weaker on the number of eggs compared to that of the Odh. -CANETE et al (1986) have found that the critical ethanol concentration where the utilization of ethanol is shifted to resistance to it lies between 5 and 7.5 %; at levels higher than this concentration, ethanol appears to be toxic rather than nutritive for the larvae. Our results on egg-to-pupa survival also suggest that the critical concentration lies between 5 % and 10% (Fig.…”

“…Consequently there must exist a critical concentration where the utilization of ethanol is shifted to the resistance to it. In certain studies this critical ethanol concentration seems to lay between 5 and 7.5 % (SANCHEZ-CANETE et al 1986).…”

Enzymatic responses of Drosophila melanogaster to long- and short-term exposures to ethanol

“…On a microgeographic scale, McKenzie and McKechnie (1979) and Gibson and Wilks (1988) have described higher alcohol tolerance inside wineries, where ethanol is present at rather high concentrations, compared to the surrounding areas, where ethanol concentrations are lower. In laboratory experiments, ethanol tolerance of larvae and adults is enhanced when they are grown on ethanol-supplemented medium (Kerver and van Delden 1985;Sanchez-Canete et al 1986;Kerver and Rotman 1987).…”