Ethanol and nitric oxide modulate expression of glucocorticoid receptor in the rat adrenal cortex

Djikić, Dragoslava; Budeč, Mirela; Vranješ-Djurić, Sanja; Todorovic, Vesna; Drndarević, Neda; Vignjević, Sanja; Mitrović, Olivera

doi:10.1016/s1734-1140(12)70884-8

Cited by 6 publications

(4 citation statements)

References 34 publications

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“…Specifically, reduced GR nuclear translocation and increased cytoplasmic retention of GR under melatonin treatment causes the up-regulation of NRF2 (Kratschmar et al 2012;Singh and Haldar 2016). Moreover, decreased liver antioxidant enzyme activity (SOD, CAT and GPx) generally contributes towards reducing total GR levels and GR activation (GR nuclear translocation) (Liu et al 2008;Skuza et al 2011;Djikic et al 2012;Wei et al 2013). In this study, the expression levels of hepatic nuclear GR proteins were negatively correlated with hepatic CAT and GPx activity.…”

Section: Discussionmentioning

confidence: 56%

Activation of glucocorticoid receptors is associated with the suppression of antioxidant responses in the liver of goats fed a high-concentrate diet

Salem

Tan

Kang

et al. 2021

Italian Journal of Animal Science

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This study investigated changes in oxidative stress and the relevant mechanisms in the liver of goats fed a high-concentrate (HC) diet for 5 weeks. Twelve goats were randomly assigned to a low-concentrate (concentrate-to-forage ¼ 55:45, LC, n ¼ 6) or HC diet (concentrate-to-forage ¼ 90:10, n ¼ 6), with dry matter as the base. Enzyme activity assays, real-time polymerase chain reaction and western blotting were used to evaluate antioxidant parameters and gene expression in the liver. Superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), total nitric oxide synthase activity and total antioxidant capacity (T-AOC) in the liver declined (p < .01) in HC-fed goats compared to those in LC-fed goats. The mRNA levels of GPX1, CAT and SOD1 were down-regulated (3.69, 47.37 and 27.61%, respectively) in HC-fed goats compared to those in LC-fed goats. Furthermore, glutathione S-transferase M1 (GSTM1) was upregulated (466.35%, p < .01) in the liver of HC-fed goats. The mRNA and protein levels of the nuclear factor E2related factor 2 (NRF2) and total glucocorticoid receptor (GR) declined (p < .05) in HC-fed goats (by 28.57, 33.1, 30.85 and 34%, respectively). However, the nuclear translocation of GR increased (p < .05; by 44.75%) in HC-fed goats. Negative correlations were detected for hepatic nuclear GR protein expression with hepatic CAT activity and GPx activity. In conclusion, feeding an HC diet to goats for 5 weeks suppressed NRF2-dependent antioxidant responses and enhanced GR nuclear translocation in the liver. HIGHLIGHTS Feeding goats high-concentrate diets suppresses NRF2-dependent antioxidant responses in liver, downregulating NRF2 and altering SOD1, GPx and CAT activity. This suppression of NRF2-dependent antioxidant responses is associated with GR activation in the liver of goats.

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Section: Discussionmentioning

confidence: 56%

Activation of glucocorticoid receptors is associated with the suppression of antioxidant responses in the liver of goats fed a high-concentrate diet

Salem

Tan

Kang

et al. 2021

Italian Journal of Animal Science

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“…Moreover, 11βHSD can effectively control the active GC concentration in local tissue (represented by cortisol in humans and corticosterone in rodents) by catalyzing reciprocal transformation between inactive and active GCs 18 . Corticoid receptors (CRs), including the mineralocorticoid receptor (MR) and glucocorticoid receptor (GR), are ligand-dependent nuclear transcription factors 19 . The insulin-like growth factor 1 (IGF1) signaling pathway is the core of the endocrine regulatory system, modulates adrenal cell proliferation, differentiation and metabolism, which play important roles in morphology and functional development 20 , 21 .…”

Section: Introductionmentioning

confidence: 99%

High-fat diet and chronic stress aggravate adrenal function abnormality induced by prenatal caffeine exposure in male offspring rats

Zheng

Ding

et al. 2017

Sci Rep

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We previously demonstrated thatprenatal caffeine exposure (PCE) suppressed fetal adrenal steroidogenesis and resulted in developmental programming changes in offspring rats. However, whether these changes play a role in adrenal corticosterone synthesis under high-fat diet (HFD) and unpredictable chronic stress (UCS) remains unknown. In present study, rat model was established by PCE (120 mg/kg.d), and male offspring were provided normal diet or HFD after weaning. At postnatal week 21, several rats fed HFD were exposed to UCS for 3 weeks and sacrificed. The results showed that compared with the corresponding control group, the serum corticosterone levels and adrenal steroid synthetase expression of the PCE offspring without UCS were reduced. Moreover, the glucocorticoid (GC)-activation system was inhibited, and insulin-like growth factor 1 (IGF1) signaling pathway expression was increased. With UCS exposure in the PCE offspring, serum corticosterone levels and adrenal steroid synthetase expression were increased, the activity of GC-activation system was enhanced, and adrenal IGF1 signaling pathway expression was decreased. Based on these findings, PCE induced adrenal hypersensitivity in adult male offspring rats, as shown by the reduced corticosterone levels under HFD conditions but significantly enhanced corticosterone levels with UCS, in which GC-IGF1 axis programming alteration may play an important role.

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“…StAR is present within mitochondria in all tissues that produce steroids [27][28][29]. StAR plays an essential role in steroid synthesis by regulating cholesterol transport from the outer to the inner mitochondrial membrane, making it available to the enzymes that convert cholesterol to the different adrenal steroids.…”

Section: Resultsmentioning

confidence: 99%

“…To our knowledge very little information exists relevant to potential paracrine or autocrine feedback of glucocorticoids within the ZF. Entering the terms glucocorticoid receptor and zona fasciculata in PubMed produced only one report [29]. This paper indicated that it was the first report to demonstrate localization and distribution of GR throughout the rat adrenal cortex and reported that endogenous nitric oxide (NO) may modulate adrenocortical expression of this steroid receptor.…”

Section: Discussionmentioning

confidence: 99%

Female baboon adrenal zona fasciculata and zona reticularis regulatory and functional proteins decrease across the life course

Huber,

Li,

Xie

et al. 2023

Preprint

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Debate exists on life-course adrenocortical zonal function trajectories. Rapid, phasic blood steroid concentration changes, such as circadian rhythms and acute stress responses, complicate quantification. To avoid pitfalls and account for life-stage changes in adrenocortical activity indices, we quantified zonae fasciculata (ZF) and reticularis (ZR) across the life-course, by immunohistochemistry of key regulatory and functional proteins. In 28 female baboon adrenals (7.5-22.1 years), we quantified 12 key proteins involved in cell metabolism, division, proliferation, steroidogenesis (including steroid acute regulatory protein, StAR), oxidative stress, and glucocorticoid and mitochondrial function. Life-course abundance of ten ZF proteins decreased with age. Cell cycle inhibitor and oxidative stress markers increased. Seven of the 12 proteins changed in the same direction for ZR and ZF. Importantly, ZF StAR decreased while ZR StAR was unchanged. Findings indicate ZF function decreased, and less markedly ZR function, with age. Causes and aging consequences of these changes remain to be determined.

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Ethanol and nitric oxide modulate expression of glucocorticoid receptor in the rat adrenal cortex

Abstract: Obtained findings are the first to demonstrate localization and distribution of the GR throughout the rat adrenal cortex and to suggest that ethanol as well as endogenous NO may modulate adrenocortical expression of this steroid receptor.

Cited by 6 publications

References 34 publications

Activation of glucocorticoid receptors is associated with the suppression of antioxidant responses in the liver of goats fed a high-concentrate diet

Activation of glucocorticoid receptors is associated with the suppression of antioxidant responses in the liver of goats fed a high-concentrate diet

High-fat diet and chronic stress aggravate adrenal function abnormality induced by prenatal caffeine exposure in male offspring rats

Female baboon adrenal zona fasciculata and zona reticularis regulatory and functional proteins decrease across the life course

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