Ethanol Consumption by Wistar Rat Dams Affects Selenium Bioavailability and Antioxidant Balance in Their Progeny

Ojeda, María Luisa; Vázquez, Beatriz Sierra; Nogales, Fátima; Murillo, María Luisa Ojeda; Carreras, Olimpia

doi:10.3390/ijerph6082139

Cited by 24 publications

(18 citation statements)

References 26 publications

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“…Moreover, our results indicated that Se is related to a high number of transport proteins for Se-Methionine (Se-Met) in the duodenum [14]. As we have previously reported, exposure of pups to ethanol during gestation and lactation causes an imbalance of Se homeostasis and a depletion in Se tissue deposits related to protein and lipid oxidation [15]. In order to improve our knowledge about the effects of ethanol on Se bioavailability in pups, we will study duodenal Se absorption.…”

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confidence: 69%

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Effects of Antioxidant Supplementation on Duodenal Se-Met Absorption in Ethanol-exposed Rat Offspring In Vivo

Nogales

Ojeda

Delgado

et al. 2011

Journal of Reproduction and Development

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Abstract. The nutritional deficiencies provoked by ethanol consumption, during gestation or lactation, can contribute to multiple birth defects in offspring. In order to improve our knowledge about selenium (Se) distribution in pups exposed to ethanol, the present study evaluated the effect of this drug on intestinal development and determined its action on duodenal absorption of selenomethionine (Se-Met). To determinate if supplementation could improve Se absorption and its serum values, we used two antioxidant supplemented regimens on dams, with selenium alone or selenium plus folic acid, and obtained six groups of pups: C (control), A (alcohol), CS (control + Se), AS (alcohol + Se), CFS (control + Se + folic acid) and AFS (alcohol + Se + folic acid). Duodenal Se-Met transport was performed using an in vivo perfusion method. Se levels were measured by graphite furnace atomic absorption spectrometry. The supplemented diets utilized had a positive influence on body growth, duodenal perimeter and Se content in ethanol-exposed pups. Ethanol exposure increased Se-Met duodenal absorption in all pups, supplemented or not, presenting the highest values of maximal velocity (V max ) compared with their control counterparts. The affinity constant (K m ) increased according to rank: A>AS>AFS groups. These results suggest that although antioxidant supplementation does not restore Se-Met absorption to normal values, it enhances the affinity of the transporters for the substrate and improves the damage caused by ethanol in the duodenal mucosa.Key words: Ethanol, Kinetic parameters, Offspring development, Selenomethionine absorption (J. Reprod. Dev. 57: [708][709][710][711][712][713][714] 2011) T he primary function of the intestinal epithelium is to absorb the small molecules produced from food digestion [1]. However, specific substances such as alcohol could alter this intestinal absorption [2,3], producing malnutrition. Numerous studies have demonstrated that chronic ethanol consumption induces morphological and biochemical alterations in the intestinal mucosal cells [4] and that some of these changes are caused by a lipid peroxidation of duodenal membrane lipids [5]. These effects of ethanol exposure are more acute during pregnancy and lactation and can contribute to a delay in offspring growth and multiple birth defects, including fetal alcohol syndrome [6]. In this context, it is especially relevant that ethanol deregulates the homeostasis of different antioxidants such as folic acid, whose deficiency is the most common sign of malnutrition in chronic alcoholism [7]. This deficiency is associated with several alterations such as Down's syndrome and defects in neural tube closure in developing embryos [1]. According to what we have previously reported [8], the effects of ethanol exposure during gestation and lactation on folic acid intestinal absorption showed that intestinal folate absorption appears to be upregulated in suckling rats. Our research group has also found, in pups exposed to ethanol and supplemented wit...

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confidence: 69%

“…We have previously found that, as a direct effect of ethanol exposure, an imbalance on Se homeostasis occurs and that there is a de-pletion of this element in different tissues in pups [15]. To increase our knowledge about Se bioavailability after ethanol exposure, we have studied duodenal Se absorption.…”

Section: Duodenal Absorption Of Selenomethioninementioning

confidence: 99%

Effects of Antioxidant Supplementation on Duodenal Se-Met Absorption in Ethanol-exposed Rat Offspring In Vivo

Nogales

Ojeda

Delgado

et al. 2011

Journal of Reproduction and Development

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“…The results of this study showed a 30 % decline in the weight of suckling rats and a decrease in growth indices (biparietal diameter and crown-rump Length) in the group on 4 % v/v ethanol compared to the control group. This could be directly related to the quality and quantity of milk produced by the dam, because consumption of ethanol during pregnancy and lactation can cause changes in macronutrients and micronutrients (Azara et al 2008) and antioxidants of milk ingredients (Ojeda et al 2009). Numerous studies have shown ethanol-induced reduction in milk production during breastfeeding, and severe malnutrition and adverse effects in neonates hence result in reduction in sucking by neonates which has an effect on the release of dam's oxytocin and prolactin (Subramanian 1995;Tavares 1999).…”

Section: Discussionmentioning

confidence: 99%

Ethanol effects on histobiochemical parameters of suckling pups borned from alcoholic rat mothers

2016

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Fetus and neonate growth retardation is one of the main characteristics of fetal alcohol syndrome (FAS) disorders. Ethanol can be transferred to the fetus through the placenta and to newborns through suckling. This study was designed to investigate the effects of consuming different doses of ethanol during lactation on enzymatic and tissue changes and growth indices in suckling rat pups. Forty-five lactating Wistar rats with nine lactating pups each were randomly allocated to three treatment groups. The two treatment groups received 2 and 4 % v/v ethanol, while the third group was the control on distilled water for 24 days. On day 25 after birth, 30 newborn rats were randomly selected from each group and serum activity of liver enzyme markers, lactate dehydrogenase, blood urea nitrogen, creatinine, and creatine phosphokinase enzymes was measured. Pathological examinations were performed on brain, liver, and kidney tissues. The obtained data was analyzed using one-way analysis of variance (ANOVA) and Dunnett's tests. Enzymatic activity of lactate dehydrogenase and creatinine was significantly (p < 0.05) higher in the rats that received alcohol as compared to the control. In histopathological examinations, different injuries were observed in kidney, liver, and brain tissues of suckling pups exposed to 4 % v/v ethanol. Consumption of alcohol in the lactating rats can cause irreparable effects on the suckling neonate.

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“…Die Verminderung des mittleren Selenspiegels bei regelmäßigem Alkoholkonsum wurde bereits in verschieden klinischen und experimentellen Settings untersucht [27,28].…”

Section: Demand For and Clinical Practice Of Complementary Selenium Tunclassified

Bedarf und Praxis der gezielten Selensubstitution im Rahmen der komplementären Behandlung des Prostatakarzinoms

Zermann

2012

Forsch Komplementmed

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Hintergrund: Die Selentherapie ist ein populäres und breit angewandtes komplementäres Therapieverfahren in der Onkologie. Ziel der vorliegenden Untersuchung war es, den Bedarf und die Indikation einer Selentherapie nach Therapie eines Postatakarzinoms zu evaluieren. Methoden: 295 konsekutive Patienten nach radikaler Prostatektomie wurden bezüglich allgemein anamnestischer, onkologischer und verhaltenstypischer Kriterien evaluiert und der Substitutionsbedarf über eine Bestimmung des Selenspiegels mittels Graphitrohr- Atomabsorptionsspektroskopie ermittelt. Ergebnisse: Der mittlere Selenspiegel war niedrig und betrug 103,4 (72,9–142,1) mg/l. Es zeigten sich Korrelationen zwischen niedrigem Selenspiegel und lokal fortgeschrittenen Tumorstadien, positivem Lymphknotenstatus, chronischem Nikotin- und Alkoholabusus und einer chronischen Mehrfachmedikation. Schlussfolgerung: Aufgrund nachgewiesener niedriger, suboptimaler Selenspiegel bei 96,3% der untersuchten Patienten und der bekannten Bedeutung einer ausreichenden ein optimal funktionierendes Immunsystem wird eine gezielte Selensubstitution im Kontext ernährungsmedizinischer Interventionen und der Beeinflussung verhaltensbedingter Risikofaktoren in der Komplementärtherapie des Prostatakarzinoms empfohlen.

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Ethanol Consumption by Wistar Rat Dams Affects Selenium Bioavailability and Antioxidant Balance in Their Progeny

Cited by 24 publications

References 26 publications

Effects of Antioxidant Supplementation on Duodenal Se-Met Absorption in Ethanol-exposed Rat Offspring In Vivo

Effects of Antioxidant Supplementation on Duodenal Se-Met Absorption in Ethanol-exposed Rat Offspring In Vivo

Ethanol effects on histobiochemical parameters of suckling pups borned from alcoholic rat mothers

Bedarf und Praxis der gezielten Selensubstitution im Rahmen der komplementären Behandlung des Prostatakarzinoms

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