Ethanol extract ofTerminalia chebulafruit protects against UVB-induced skin damage

Abstract

Context: The fruit of Terminalia chebula Retz. (Combretaceae) has been used for several therapeutic purposes in Thai folk medicines. Currently, the ethanol extracts containing antioxidant compounds have shown the ability to promote collagen synthesis.Objective: This purpose of this work was to study the effects of the ethanol extract from T. chebula fruit on the inhibition of cutaneous photodamage. Materials and methods: The viability of human skin fibroblasts after incubation with T. chebula at concentration 0.5-50 lg/mL for 24, 48 and 72 h was assessed by using sodium 3 0 -[(phenyl-amino)-carbonyl]-3,4,tetrazolium-bis(4-methoxy-6-notro)benzene-sulphonic acid hydrate (XTT). The levels of type I procollagen and matrix metalloproteinases (MMP)-1 and MMP-13 produced by UVB-irradiated fibroblasts were determined by ELISA. Skin thickness and collagen content caused by long-term UVB irradiation in male ICR mice were determined from haematoxylin and eosin stained tissue sections and spectrophotometric measurement of hydroxyproline. Results: The extract (0.5-50 lg/mL) had no effect on cell viability or morphology of the human fibroblasts. In vitro studies showed that the T. chebula extract reduced the UVB-induced MMP-1 and MMP-13 expression, whereas an increased production of type I procollagen was observed. In a UVB-irradiated animal model, male ICR mice with hair shaved were chronically exposed to UVB which lead to epidermal thickness and loss of hydroxyproline. However, these effects were fully prevented by the topical application of the T.

Evaluation of cytotoxic, immunomodulatory and antibacterial activities of aqueous extract from leaves of Conocarpus erectus Linnaeus (Combretaceae)

“…accelerated wound healing in burn wounds of rats, semipurified fraction containing gallic acid from T. chebula Retz. showed antiageing activity and ethanol extract from fruit of this same plant protected skin against UVB photodamage . The immunomodulatory results found in the present work stimulate future studies evaluating the effectiveness of AELCe in wound healing.…”

“…showed antiageing activity [40] and ethanol extract from fruit of this same plant protected skin against UVB photodamage. [41] The immunomodulatory results found in the present work stimulate future studies evaluating the effectiveness of AELCe in wound healing.…”

Wound healing activity of Streptocaulon juventas root ethanolic extract

“…The Sj extract was prepared as described in previous studies . Dried Sj root powder was extracted in 70% ethanol for 48 hours (w/v = 1 : 10) . The extract was then condensed by a rotary vacuum evaporator and freeze dried.…”

Hom–Kularb–Dang Rice Bran Extract for the Prevention of UVB-Damage Against Human Skin Fibroblast

“…DPPH radical scavenging activity was determined using the method previously described (Itsarasook et al, 2014;Phetdee et al, 2014;Yakaew et al, 2016). Briefly, 1 ml of 0.2 mM DPPH solution (Sigma-Aldrich Chemic GmbH, Steinheim, Germany) was mixed with 0.5 ml of various concentrations of the sample of extract previously dissolved in methanol.…”

“…Cell viability test. Cell viability was assessed using sodium 3′-[(phenylamino)-carbonyl]-3,4,tetrazolium-bis (4-methoxy-6-notro) benzene-sulfonic acid hydrate ( XTT, Boehringer Mannheim, Mannheim, Germany) assay (Inpanya et al, 2012;Phetdee et al, 2014;Yakaew et al, 2016). The cells were seeded at 1 x 10 4 cells/ cm 2 into each well of 96 well plates.…”