Ethanol extract of  Kalopanax septemlobus  leaf inhibits HepG2 human hepatocellular carcinoma cell proliferation  via  inducing cell cycle arrest at G 1  phase

Park, Cheol; Jeong, Ji-Suk; Jeong, Jin‐Woo; Kim, Sung Ok; Kim, Yong Joo; Kim, Gi‐Young; Hong, Su‐Hyun; Choi, Yung Hyun

doi:10.1016/j.apjtm.2016.03.003

Cited by 7 publications

(3 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Chinese medicinal herbs have been used for the treatment of various diseases in China and Southeast Asia (23). Also, many studies have focused on the anticancer effects of phytochemicals which is contained in natural plant (3,(7)(8)(9)(10). In the present study, we examined whether ethanol extracts from C. monnieri (CME)-induced apoptotic and cell cycle arrest effects were occurred by p53-dependent or p53-independent mechanism in HepG2 (wild-type p53) and Hep3B (p53-null) hepatocellular carcinoma cells.…”

Section: Discussionmentioning

confidence: 99%

“…A previous study reported that bioactive compounds from natural plant extracts play a crucial role as potential therapeutic agents (6). Many plant extracts induce anticancer effects such as anti-proliferative and anti-metastatic effects and cell cycle arrest in various cancer cell lines (7)(8)(9). Moreover, the anticancer activity of natural plant extracts has been investigated in HepG2 and Hep3B hepatocellular carcinoma cells (3,10).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Ethanol extract from Cnidium monnieri (L.) Cusson induces cell cycle arrest and apoptosis via regulation of the p53‑independent pathway in HepG2 and Hep3B hepatocellular carcinoma cells

Lim

Kim

et al. 2017

Mol Med Report

View full text Add to dashboard Cite

Cnidium monnieri (L.) Cusson is a frequently used traditional Chinese medicine that treats gynecological diseases and carbuncles. However, the mechanism of action of C. monnieri remains to be fully elucidated. The present study examined the cell cycle arrest and apoptotic effects resulting from ethanol extract of C. monnieri (CME) in HepG2 (wild‑type p53) and Hep3B (p53‑null) hepatocellular carcinoma cells. An MTT assay was used to confirm the anti‑proliferative effect of CME. The cells were stained with Hoechst 33342 or propidium iodide. It was demonstrated that proliferation of HepG2 cells was suppressed by CME. Cell cycle arrest occurred in the G1 phase following treatment with CME and the number of apoptotic bodies was increased. The expression levels of cell cycle‑associated proteins, including protein kinase B (Akt), glycogen synthase kinase‑3β (GSK‑3β), p53, cyclin E and cyclin‑dependent kinase 2 (CDK2) were determined by western blot analysis. The protein levels of phosphorylated (p)‑Akt, p‑GSK‑3β, p‑MDM2 and cyclin E were decreased, whereas the protein levels of p53, p21 and p‑CDK2 (Thr14/Tyr15) were increased following treatment with CME. Furthermore, treatment or co‑treatment with LY294002 (phosphoinositide‑3‑kinase/Akt inhibitor) or Pifithrin‑α (p53 inhibitor) with CME resulted in CME‑induced G1 arrest which occurred through the p53‑independent signaling pathway in hepatocellular carcinoma cells. In conclusion, CME induces G1 arrest and apoptosis via the Akt/GSK‑3β signaling pathway which is regulated by MDM2‑induced degradation of p21, rather than p53.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ethanol extract from Cnidium monnieri (L.) Cusson induces cell cycle arrest and apoptosis via regulation of the p53‑independent pathway in HepG2 and Hep3B hepatocellular carcinoma cells

Lim

Kim

et al. 2017

Mol Med Report

View full text Add to dashboard Cite

show abstract

“…are sold at local markets in Korea ( Table 1 ). The extracts and compounds obtained from traditional GLVs (1–18) have been shown to possess antioxidant [ 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 ], anticancer [ 18 , 23 , 24 , 25 , 26 ], antiinflammation [ 20 , 25 , 27 , 28 ], anti-melanogenic [ 29 , 30 ], anti-fatigue [ 31 ], anti-obesity [ 32 , 33 ], antidiabetic [ 34 ], and immunostimulatory [ 35 ] activities. GLVs have abundant phytopigments.…”

Section: Introductionmentioning

confidence: 99%

Carotenoid and Tocopherol Profiling in 18 Korean Traditional Green Leafy Vegetables by LC-SIM-MS

Lee

Sivanesan

et al. 2023

Foods

View full text Add to dashboard Cite

Fruits and vegetables are a vital source of redox-active phytochemicals in the diet. Traditional green leafy vegetables (GLVs) are a rich source of carotenoids, dietary fiber, minerals, phenols, vitamins, and tocopherols and are commonly consumed in rural areas worldwide. In traditional Korean medicine, many GLVs are used to treat various ailments. However, data on the carotenoid and tocopherol content of many traditional GLVs consumed in the Republic of Korea are insufficient. The current work aims to compare the carotenoid and tocopherol profiles of 18 traditional GLVs by utilizing a single ion monitoring LC-MS approach to identify the potential GLVs for commercial cultivation and healthy diet formulations. Among the traditional GLVs investigated, (all-E)-lutein was the most abundant carotenoid, ranging from 44.4% in Glehnia littoralis to 52.1% in Heracleum moellendorffii. It was followed by (all-E)-violaxanthin and (all-E)-β-carotene. The highest contents of (all-E)-violaxanthin (75.6 µg/g FW), 9-Z-neoxanthin (48.4 µg/g FW), (all-E)-luteoxanthin (10.8 µg/g FW), (all-E)-lutein (174.1 µg/g FW), total xanthophylls (310.5 µg/g FW), (all-E)-β-carotene (69.6 µg/g FW), and total carotenoids (380.1 µg/g FW) were recorded in Pimpinella brachycarpa. Surprisingly, Taraxacum mongolicum also showed the highest contents of (all-E)-violaxanthin, (all-E)-lutein, and total carotenoids, which were statistically non-significant (p > 0.05, Tukey HSD) with P. brachycarpa. The highest concentration of (all-E)-zeaxanthin (14.4 µg/g FW) was recorded in Solidago virga-aurea. Among the studied herbs, 13.9 (H. moellendorffii)–133.6 µg/g FW (Toona sinensis) of α-tocopherol was recorded. Overall, the results suggest that P. brachycarpa and T. mongolicum are rich sources of carotenoids. On the other hand, T. sinensis is a rich source of α-tocopherol. These GLVs can be utilized in the diet to enhance the intake of health-beneficial carotenoids and α-tocopherol.

show abstract

Green synthesis of zinc oxide nanoparticles using Brassica oleracea var. botrytis leaf extract: Photocatalytic, antimicrobial and larvicidal activity

et al. 2023

View full text Add to dashboard Cite

Ethanol extract of Kalopanax septemlobus leaf inhibits HepG2 human hepatocellular carcinoma cell proliferation via inducing cell cycle arrest at G 1 phase

Abstract: Overall, our findings suggest that EEKS may be an effective treatment for liver cancer through suppression of cancer cell proliferation via G1 cell cycle arrest. Further studies are required to identify the active compounds in EEKS.

Cited by 7 publications

References 29 publications

Ethanol extract from Cnidium monnieri (L.) Cusson induces cell cycle arrest and apoptosis via regulation of the p53‑independent pathway in HepG2 and Hep3B hepatocellular carcinoma cells

Ethanol extract from Cnidium monnieri (L.) Cusson induces cell cycle arrest and apoptosis via regulation of the p53‑independent pathway in HepG2 and Hep3B hepatocellular carcinoma cells

Carotenoid and Tocopherol Profiling in 18 Korean Traditional Green Leafy Vegetables by LC-SIM-MS

Green synthesis of zinc oxide nanoparticles using Brassica oleracea var. botrytis leaf extract: Photocatalytic, antimicrobial and larvicidal activity

Contact Info

Product

Resources

About