Ethanol extract of Ocimum sanctum exerts anti-metastatic activity through inactivation of matrix metalloproteinase-9 and enhancement of anti-oxidant enzymes

Can Ocimum basilicum L. and Ocimum tenuiflorum L. in vitro culture be a potential source of secondary metabolites?

“…Eugenol quantification has been reported using several methods such as LC-MS, GC-MS and HPLC with different detectors (Domlur Thyagaraj et al, 2013;Kim et al, 2010). Moreover several HPLC/mobile phase combinations have also been reported for eugenol quantification (Chang et al, 2011;Domlur Thyagaraj et al, 2013).…”

Ocimum gratissimumretards breast cancer growth and progression and is a natural inhibitor of matrix metalloproteases

“…46 Ethanol extracts of O. sanctum significantly inhibited cell adhesion and invasion as well as activities of MMP-9, but not MMP-2, in mouse Lewis lung carcinoma (LLC) cells. 4 However, in none of these studies an analysis of enzymatic activities of MMP-2 and MMP-9 was performed.…”

“…3 Ethanolic extracts of Ocimum sanctum were reported to be cytotoxic to mouse Lewis lung carcinoma (LLC) cells and reduced number of tumor nodule formation in LLC-injected mice. 4 Essential oils isolated from Ocimum viride showed cytotoxic and apoptotic activities toward the colonic carcinoma cells COLO 205. 5 Previously, we compared three species of Ocimum, O. gratissimum, O. sanctum (green) and O. sanctum (purple) and examined their effect on breast cancer and endothelial cell migration.…”

Herbal Antioxidants as Rejuvenators in Alternative Medicine

“…Another study indicated that 6-gingerol has been reported to inhibit NO synthesis and protect against peroxynitrite-mediated damage (Ippoushi et al, 2003). Apart from these, several studies investigating the anti-cancer potential of ginger have reported the involvement of signaling molecules such as NF-κB, TNF-, AKT, ERK1/2, p38 MAPK, MMP-2 and MMP-9 in mediating the cellular responses to various phytochemicals of ginger (Rhode et al, 2007;Habib et al, 2008;Hung et al, 2009;Yodkeeree et al, 2009;Kim et al, 2010).…”

“…The stress alleviating potential of Ocimum sanctum extract is also reflected by its efficacy in attenuating the immunological changes induced by noise exposure (Archana and Namasivayam, 2000). In addition, tulsi has been reported to possess several other important medicinal properties (Singh et al, 1996;Mediratta et al, 2002;Adhvaryu et al, 2007;Kim et al, 2010) that strengthen its potential to deal with aging and oxidative stress. Thus, Tulsi is capable of promoting a healthy aging due to its multiple but complimentary and synergistic medicinal properties which can combat a diverse spectrum of disorders associated with aging.…”