Ethanol extract of Peperomia pellucida (Piperaceae) promotes fracture healing by an anabolic effect on osteoblasts

Ngueguim, Florence Tsofack; Khan, Mohd Parvez; Donfack, Jean Hubert; Tewari, Deepshikha; Dimo, Théophile; Kamtchouing, P.; Maurya, Rakesh; Chattopadhyay, Naibedya

doi:10.1016/j.jep.2013.03.063

Cited by 26 publications

(23 citation statements)

References 19 publications

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“…White needles was found in the fourth fraction, then dissolved with methanol to solve impurities, pure crystal (485 mg) get from recrystallized used hexane and chloroform. Purity test was performed by TLC using hexane and ethyl acetate solvent (4:1) and compared with standard stigmasterol, further determined its melting point, FT-IR and 1 H, 13 C NMR its marked as compound 1. The melting point was determined using a micro melting point measurement (Fisher Scientific (Fisher Scientific).…”

Section: Extraction and Isolationmentioning

confidence: 99%

“…Also get the yield 16 mg pure compound 2. Further determined its melting point, FT-IR and 1 H, 13 C NMR one and two dimension. Ethyl acetate fraction (2.8 g) were subjected to silica gel G60 column chromatography using mobile phase n-hexane-ethyl acetate and methanol by gradient afforded 13 fractions.…”

Section: Extraction and Isolationmentioning

confidence: 99%

“…Compound 3 deducted by spectroscopic data FT-IR, LC-MS, 1 H and 1 NMR 1D and 2D. IR spectrum were taken FT-IR Prestige-21, Shimadzu, NMR spectra of 1 H, 13 C, HMQC and HMBC were measured using an Inova Plus, Unity NMR 500 at 500 MHz ( 1 H) and 125 MHz ( 13 C) with TMS as an internal standard in measurement NMR spectrometer. LC-MS analysis was performed using a Mariner spectrometry equipped with a binaru pump.…”

Section: Extraction and Isolationmentioning

confidence: 99%

“…(Table 3). On the basis of IR, 1 H-NMR and 13 C-NMR spectra data and the other physical properties the isolate pure compound 3 were identified and established as β-sitosterol-D-glucopyranoside as shown in Figure 3.…”

Section: Extraction and Isolationmentioning

confidence: 99%

“…et al, 2013 [12] reported that dillapiole was identified as the most active compound in the dichloromethane extract and also reported the gastro protector activity of dillapiole. Ethanolic extract of P. pellucida accelerates fracture repairment in rats via stimulatory effects on osteoblast differentiation and mineralisation [13]. In this research have been done an isolation of others compounds which furthermore research will testing for the bioactivity.…”

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confidence: 99%

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Isolation and Characterization Compounds From Hexane and Ethyl Acetate Fractions of Peperomia pellucida L.

Hartati¹,

Angelina

Dewiyanti

et al. 2015

JTLS

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Peperomia pellucida was used traditionally in Indonesia for health treatment: wounds, boils, pimples, abscesses, abdominal pain, colic, gout, kidney, rheumatic pain, fatigue headache, furuncles, conjunctivitis and anti dermatogenic and also for dengue treatment. The isolation compounds from hexane and ethyl acetate fractions of Peperomia pellucida L. are conducted by maceration of the dry herbs sample with methanol and partition with hexane, ethyl acetate, butanol and water. The hexane and ethyl acetate fractions were fractionated by gravitation column chromatography and eluted successively with hexane, ethyl acetate and methanol by the gradient. The structure was elucidated base on spectroscopy data of NMR proton and carbon for one and two dimension, LC-MS and FT-IR. The isolation founded three compounds are stigmasterol, analogue of pheophytin and β-sitosterol-D-glucopyranoside.

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Section: Extraction and Isolationmentioning

confidence: 99%

Section: Extraction and Isolationmentioning

confidence: 99%

Section: Extraction and Isolationmentioning

confidence: 99%

Section: Extraction and Isolationmentioning

confidence: 99%

mentioning

confidence: 99%

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Isolation and Characterization Compounds From Hexane and Ethyl Acetate Fractions of Peperomia pellucida L.

Hartati¹,

Angelina

Dewiyanti

et al. 2015

JTLS

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What is the role of bosentan in healing of femur fractures in a rat model?

et al. 2014

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The purpose of this study was to examine the effects bosentan (which is a strong vasoconstrictor) on bone fracture pathophysiology, and investigate the roles of the nonselective endothelin 1 receptor blocker bosentan on the bone fractures formed in rats through radiographic, histopathologic, and immunohistochemical methods. The rats were divided into three groups (six rats in each group): a femoral fracture control group, a femoral fracture plus bosentan at 50 mg/kg group, and a femoral fracture plus bosentan at 100 mg/kg group. The femoral fracture model was established by transversely cutting the femur at the midsection. After manual reduction, the fractured femur was fixed with intramedullary Kirschner wires. The radiographic healing scores of the bosentan 100 and 50 mg/kg groups were significantly better that those of the fracture control group. The fracture callus percent of new bone in the bosentan 100 mg/kg group was significantly greater than that in the control group. Also, semiquantitative analysis showed higher positive vascular endothelial growth factor and osteocalcin staining and lower positive endothelin receptor type A staining in the treatment groups than in the control group. Bosentan treatment also decreased tissue endothelin 1 expression relative to that in the fracture control group. As a result of our study, the protective effect of bosentan was shown in experimental femoral fracture healing in rats by radiographic, histopathologic, and molecular analyses.

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