Ethanol extract of Poria cocos reduces the production of inflammatory mediators by suppressing the NF-kappaB signaling pathway in lipopolysaccharide-stimulated RAW 264.7 macrophages

Jeong, Jin‐Woo; Lee, Hye Hyeon; Han, Min; Kim, Gi‐Young; Hong, Su Hyun; Park, Cheol; Choi, Yung Hyun

doi:10.1186/1472-6882-14-101

Cited by 54 publications

(31 citation statements)

References 37 publications

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“…4 ), which is consistent with results of our previous studies in RAW 264.7 macrophages. 27 – 30 SF also has ability to inhibit the LPS-induced nuclear translocation of NF- κ B p65 and the degradation of I κ B-α. These effects might arise through suppression of the phosphorylation and proteasome-mediated degradation of its inhibitor I κ B-α, resulting in lower levels of NF- κ B transactivation.…”

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory Effects of Schisandra chinensis (Turcz.) Baill Fruit Through the Inactivation of Nuclear Factor-κB and Mitogen-activated Protein Kinases Signaling Pathways in Lipopolysaccharidestimulated Murine Macrophages

Kang

Han

Hong

et al. 2014

Journal of Cancer Prevention

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Background:Schisandrae Fructus, the dried fruit of Schisandra chinensis (Turcz.) Baill. (Magnoliaceae), is widely used in traditional medicine for the treatment of a number of chronic inflammatory diseases. This study examined the anti-inflammatory effects of Schisandrae Fructus ethanol extract (SF) on the production of pro-inflammatory substances in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages.Methods:To measure the effects of SF on pro-inflammatory mediator and inflammatory cytokine’s expression and production in RAW 264.7 cells, we used the following methods: cell viability assay, Griess reagent assay, enzyme-linked immunosorbent assay, reverse transcriptase-polymerase chain reaction, Western blotting analysis and immunofluorescence staining.Results:Stimulation of the RAW 264.7 cells with LPS caused an elevated production of nitric oxide (NO), tumor necrosis factor α (TNF-α) and interleukin (IL)-1β, which was markedly inhibited by the pretreatment with SF without causing any cytotoxic effects. SF also inhibited the expression of inducible NO synthase, TNF-α, and IL-1β protein and their mRNAs in LPS-stimulated RAW 264.7 cells. Furthermore, SF attenuated LPS-induced nuclear translocation of nuclear factor-κB (NF-κB) by reducing inhibitory-κB degradation, and reduced the phosphorylation of mitogen-activated protein kinases (MAPKs), implying that SF regulated LPS-induced NF-κB-dependent inflammatory pathways through suppression of MAPKs activation.Conclusions:SF may be useful for the treatment of various inflammatory diseases.

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Section: Discussionmentioning

confidence: 99%

Anti-inflammatory Effects of Schisandra chinensis (Turcz.) Baill Fruit Through the Inactivation of Nuclear Factor-κB and Mitogen-activated Protein Kinases Signaling Pathways in Lipopolysaccharidestimulated Murine Macrophages

Kang

Han

Hong

et al. 2014

Journal of Cancer Prevention

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“…Here, in this study, we found that the integrity of intestinal villi and the expression of ZO-1 and E-cadherin in the rats of BPHW decoction were ameliorated, while the intestinal expression of TLR-4 and NF-κB of rats, as well as the contents of TNF-α, IL-1β, and IL-6 was also significantly lower than those in the model group. Moreover, it has also been reported that Astragalus [18] and Poria [19] can protect lipopolysaccharide-induced intestinal mucosal injury by reducing the production of inflammatory cytokines, such as IL-1β, and TNF-α through inhibiting the NF-κB signaling pathway. Atractylodes [20] and Pinellia ternata [21] can promote cell proliferation and repair by inhibiting the proapoptotic gene (Bax) expression through polyaminemediated Ca 2+ signaling pathway.…”

Section: Discussionmentioning

confidence: 98%

BuPiHeWei Decoction Ameliorates 5-Fu-Induced Intestinal Mucosal Injury in the Rats by Regulating the TLR-4/NF-κB Signaling Pathway

Sun

Wang

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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BuPiHeWei (BPHW) decoction, a classic Traditional Chinese Medicinal (TCM) prescription, has been widely used in clinical practice to relieve digestive symptoms caused by chemotherapy, such as diarrhea and vomiting. The present study aimed to investigate whether BPHW decoction exerted a protective role in the 5-Fu-induced intestinal mucosal injury in the rats by regulating the mechanisms of TLR-4/NF-κB signaling pathway. There were 35 Sprague Dawley rats randomly divided into four groups: normal control group, 5-Fu group, 5-Fu + BPHW decoction group (10.5 g/kg, for five continuous days), and 5-Fu + Bacillus licheniformis capsule group (0.2 g/kg, for five continuous days). Animal models were established by intraperitoneal injection of 5-Fu (30 mg/Kg, for five consecutive days). At the end of the treatment period, body weight, diarrhea score, and histological examination were examined. Furthermore, the expression of TLR-4/NF-κB pathway was detected to reveal its mechanism. The results showed that BPHW decoction effectively reduced diarrhea score and increased body weight and height of villi after 5-Fu chemotherapy. In addition, BPHW decoction could significantly inhibit the expression of TLR-4, NF-κB, and inflammatory factors (including TNF-α, IL-1β, and IL-6) in the intestine, and the efficacy was significantly higher than that of Bacillus licheniformis capsule. In summary, BPHW decoction might be considered an effective drug to alleviate intestinal mucosal injury in the rats induced by 5-Fu.

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“…NFKBIA, known as I-kappa-B-alpha (I-κBα), which inhibits the activity of dimeric NF-kappa-B/REL complexes, has been reported as a target for glucocorticoid-mediated immunosuppression [44]. It has been demonstrated that the extract of PC can reduce the production of inflammatory mediators by suppressing the NF-κB signaling pathway [45]. Although all TLRs could lead to NF-κB activation, we found that ZDW might be more sensitive to an LPS-induced TLR4/NF-κB signaling pathway.…”

Section: Discussionmentioning

confidence: 99%

Potential Molecular Mechanisms of Zhibai Dihuang Wan in Systemic Lupus Erythematosus Based on Network Biology

Yang

Xie

Zhong

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Systemic lupus erythematosus (SLE) is a refractory autoimmune disease. Zhibai Dihuang Wan (ZDW) has frequently been used for treating SLE in China and been proved to have a prominent role in decreasing SLE patients’ morality rate. However, the active substances in ZDW and the molecular mechanisms of ZDW in SLE remain unclear. This study identified the bioactive compounds and delineated the molecular targets and potential pathways of ZDW by using a network biology approach. First, we collected putative targets of ZDW based on TCMSP, GeneCards, and STITCH databases and built a network containing the interactions between the putative targets of ZDW and known therapeutic targets of SLE. Then, the key hubs were imported to DAVID Bioinformatics Resources 6.7 to perform gene ontology biological process (GOBP) and pathway enrichment analysis. A total of 95 nodes including 73 putative targets of ZDW were determined as major hubs in terms of their node degree. The results of GOBP and pathway enrichment analysis indicated that putative targets of ZDW mostly were involved in various pathways associated with inflammatory response and apoptosis. More importantly, eleven putative targets of ZDW (CASP3, BCL2, BAX, CYCS, NFKB1, NFKBIA, IL-6, IL-1β, PTGS2, CCL2, and TNF-α) were recognized as active factors involved in the main biological functions of treatment, implying the underlying mechanisms of ZDW acting on SLE. This study provides novel insights into the mechanisms of ZDW in SLE, from the molecular level to the pathway level.

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Ethanol extract of Poria cocos reduces the production of inflammatory mediators by suppressing the NF-kappaB signaling pathway in lipopolysaccharide-stimulated RAW 264.7 macrophages

Cited by 54 publications

References 37 publications

Anti-inflammatory Effects of Schisandra chinensis (Turcz.) Baill Fruit Through the Inactivation of Nuclear Factor-κB and Mitogen-activated Protein Kinases Signaling Pathways in Lipopolysaccharidestimulated Murine Macrophages

Anti-inflammatory Effects of Schisandra chinensis (Turcz.) Baill Fruit Through the Inactivation of Nuclear Factor-κB and Mitogen-activated Protein Kinases Signaling Pathways in Lipopolysaccharidestimulated Murine Macrophages

BuPiHeWei Decoction Ameliorates 5-Fu-Induced Intestinal Mucosal Injury in the Rats by Regulating the TLR-4/NF-κB Signaling Pathway

Potential Molecular Mechanisms of Zhibai Dihuang Wan in Systemic Lupus Erythematosus Based on Network Biology

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