Ethanol extract of propolis-bovine bone graft combination as a prospective candidate for socket preservation: Enhancing BMP7 and decreasing NFATc1

Kresnoadi, Utari; Widjaja, Jennifer; Laksono, Harry

doi:10.1016/j.sdentj.2021.05.003

Cited by 4 publications

(3 citation statements)

References 21 publications

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“…Paraffin blocks were cut to a thickness of four microns and placed in an object glass. 17 The tissue deparaffinization process was completed using a solution of xylol, ethanol, and alcohol. Processing of the tissues was continued with a 3.3'-diaminobenzidine (DAB) staining kit (Pierce™ DAB Substrate Paint Kit 34002, Thermofisher™, Massachusetts, United States).…”

Section: Methodsmentioning

confidence: 99%

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Evaluation of BSP and DMP1 in hydroxyapatite crab shells used for dental socket preservation

Kamadjaja

Salim²,

Waluyo³

et al. 2023

Dent. J.

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Background: Bone resorption due to tooth extraction leads to unpredictable bone volume for future prosthetics. Crab shells were promoted as a solution to prevent bone resorption, along with an effort to reduce biological waste. Purpose: This study aimed to analyze the expression of bone sialoprotein (BSP) and dentine matrix protein-1 (DMP1) in the wound healing process in tooth-extraction sockets after applying a crab shell-derived hydroxyapatite scaffold. Methods: The subjects (28 Cavia cobaya) were divided into control and treatment groups. The control group was left untreated, while the treatment group received a hydroxyapatite scaffold of Portunus pelagicus shell in the tooth socket. The expression of BSP and DMP1 was determined by immunohistochemical staining on days 7 and 14. One-way analysis of variance and Tukey’s honest significance difference test were used to find the groups with the most significant difference. Results: The highest mean expression of BSP and DMP1 was in the day 14 treatment group, while the lowest was in the day 7 control group. Conclusion: Administering hydroxyapatite scaffold derived from the Portunus pelagicus shell to the post-extraction sockets increased the expression of both BSP and DMP1.

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Section: Methodsmentioning

confidence: 99%

“…The observation area was specified as the apical third of the socket. 17 The IBM Statistical Package for the Social Sciences, Statistics for Windows, version 24.0. (Armonk, NY: IBM Corp) was used in this study.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of BSP and DMP1 in hydroxyapatite crab shells used for dental socket preservation

Kamadjaja

Salim²,

Waluyo³

et al. 2023

Dent. J.

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“…16 IHC staining was used for examining BMP-2, OCN, OSX, and RUNX2 assay. 1,16,20,21 For the examination of BMP-2, OSX, OCN, and RUNX2 expressions, OB, and OC, we used a 1,000Â magnification light microscope in 20 microscopic fields. The mean results per microscopic field were tabulated and analyzed with SPSS version 25.…”

Section: Methodsmentioning

confidence: 99%

The Osteogenesis Mechanisms of Dental Alveolar Bone Socket Post Induction with Hydroxyapatite Bovine Tooth Graft: An Animal Experimental in Rattus norvegicus Strain Wistar

et al. 2022

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Objectives Surgical endodontics (hemisection) commonly involves the alveolar bone socket and the periradicular tissue. In today's era, optimizing the bone healing process is updated by using bone graft induction. This study explores the mechanisms of bone healing of the alveolar bone socket post-dental extraction of Wistar rats after administration of a bovine tooth graft (hydroxyapatite bovine tooth graft [HAp-BTG]). Materials and Methods Fifty Wistar rats were randomly selected into two groups, control and treatment, and into five subgroups on days 3, 7, 14, 21, and 28. The postextraction socket was filled with polyethylene glycol (PEG) as the control and PEG + HAp-BTG as the treatment group. On days 3, 7, 14, 21, and 28, Wistar rats were sacrificed, mandibles were taken, paraffin blocks were made, cut 4 µm thick, and made into glass preparations for microscopic examination. The variable analysis was performed by staining hematoxylin-eosin for osteoblasts (OBs) and osteoclasts (OCs) and immunohistochemistry for runt-related transcription factor 2 (RUNX2), osterix (OSX), osteocalcin (OCN), bone morphogenic protein (BMP) 2. We analyzed the expressed cell count per microscope field. Results In general, the number of cell expressions in the treatment group was significantly higher and faster, except for significantly lower OC. The high variables peak occurred on day 14 for RUNX2 and OCN, on day 7 for OSX, while OB significantly increased on day 21 and remained until day 28. The decrease of OC cells occurred on day 7 and remained low until 28 days. BMP2 was first dominantly induced by HAp-BTG, then the others. Conclusion HAp-BTG can induce higher and faster bone healing biomarkers. BMP2 is the dominant first impacted. On the 28th day, it did not significantly express the suppression of OC by OB, which entered the bone formation and remodeling step.

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BMP-7 Expression After Induction of Hydroxyapatite Bovine Tooth Graft Gel as A Socket Preservation of The Alveolar Bone (Laboratory Experimental of The Alveolar Bone)

Zubaidah,

Namira,

Aryandhita

et al. 2023

CDJ

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Background: Hemisection is an endodontic surgical procedure that can leave alveolar bone defects. Changes in the dimensions of the alveolar bone can be minimized by preserving the alveolar bone socket using a bone graft. Induction of Hydroxyapatite Bovine Tooth Graft (HAp-BTG) in the alveolar bone socket is expected to increase the expression of BMP-7 which is a growth factor in osteogenesis. Purpose: To describe the increase in BMP-7 expression after induction of hydroxyapatite bovine tooth graft gel as a socket preservation of the alveolar bone of Wistar rats on the 14th and 28th days. Methods: 28 Wistar rats were extracted on the lower left incisor. Post-extraction sockets were induced by Polyethylene Glycol (PEG) as the control group and HAp-BTG + PEG as the treatment group. On the 14th and 28th day the Wistar rats were euthanized and the mandibles were taken. By using immunohistochemical staining on anatomic histopathological examination, BMP-7 expression was observed using a microscope with 1000× magnification. The research results were analyzed using One Way ANOVA. Results: There was a significant increase in BMP-7 expression between the control group and the treatment group. Conclusion: BMP-7 expression after induction of hydroxyapatite bovine tooth graft gel as a socket preservation of the alveolar bone of Wistar rats increased on the 14th and 28th days.

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Ethanol extract of propolis-bovine bone graft combination as a prospective candidate for socket preservation: Enhancing BMP7 and decreasing NFATc1

Cited by 4 publications

References 21 publications

Evaluation of BSP and DMP1 in hydroxyapatite crab shells used for dental socket preservation

Evaluation of BSP and DMP1 in hydroxyapatite crab shells used for dental socket preservation

The Osteogenesis Mechanisms of Dental Alveolar Bone Socket Post Induction with Hydroxyapatite Bovine Tooth Graft: An Animal Experimental in Rattus norvegicus Strain Wistar

BMP-7 Expression After Induction of Hydroxyapatite Bovine Tooth Graft Gel as A Socket Preservation of The Alveolar Bone (Laboratory Experimental of The Alveolar Bone)

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