Ethanol-extracted Cameroonian propolis exerts estrogenic effects and alleviates hot flushes in ovariectomized Wistar rats

Zingue, Stéphane; Nde, Chantal Beatrice Magne; Michel, Thomas; Ndinteh, Derek Tantoh; Tchatchou, Jules; Adamou, Moïse; Fernández, Xavier; Fohouo, Fernand-Nestor Tchuenguem; Clyne, Colin; Njamen, Dieudonné

doi:10.1186/s12906-017-1568-8

Cited by 31 publications

(38 citation statements)

References 47 publications

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“…Polyphenols were reported as ligands of estrogen receptors (ERs) due to their ER binding affinity, and are able to target hormone-related cancers [67]. Furthermore, emerging evidence shows that propolis has an estrogenic effect through activation of ERs [68,69]. Therefore, our results for female mice may support the idea that the microbiota is affected by the hormone status of the mice, especially by estrogen.…”

Section: Discussionsupporting

confidence: 77%

Chinese Propolis Prevents Obesity and Metabolism Syndromes Induced by a High Fat Diet and Accompanied by an Altered Gut Microbiota Structure in Mice

Zheng

Tao

et al. 2020

Nutrients

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The increasing incidence of obesity poses a great threat to public health worldwide. Recent reports also indicate the relevance of obesity in metabolic diseases. Chinese propolis (CP), as a well-studied natural nutraceutical, has shown a beneficial effect on alleviating diabetes mellitus. However, few studies have investigated the effect of CP on weight management and energy balance. We examined the beneficial effects of dietary CP on weight in high-fat diet-fed female and male mice and determined whether CP alters gut microbiota. In this study, dietary CP supplementation reduces body weight and improves insulin resistance in high-fat diet (HFD)-fed mice in a dose-dependent manner. CP treatment also reverses liver weight loss and triglyceride accumulation in association with hepatic steatosis. The 16S rRNA analysis of gut microbiota demonstrated that CP treatment modulates the composition in HFD-fed mice. Our study also suggests that male mice were more sensitive to CP treatment than female mice. Taken together, CP supplementation reduces weight gain and reverses gut microbiome dysbiosis induced by HFD. Further, the effects of CP treatment on metabolic biomarkers and microbiome structure differ by gender.

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Section: Discussionsupporting

confidence: 77%

Chinese Propolis Prevents Obesity and Metabolism Syndromes Induced by a High Fat Diet and Accompanied by an Altered Gut Microbiota Structure in Mice

Zheng

Tao

et al. 2020

Nutrients

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“…These effects were attenuated by co-treatment with the anti-estrogen compound fulvestrant, reflecting the estrogenic activity of the R. undulatum extract and its ingredients, which were mediated via the ER. Therefore, R. undulatum extracts showed estrogenic potency, which is in line with the results of previous studies [ 25 , 26 ]. In addition, we identified new effective compounds (aloe emodin, rhapontigenin, and chrysophanol 1- O -β- d -glucopyranoside) in this study.…”

Section: Discussionsupporting

confidence: 92%

“…Breast cancer comprises cells with different types of receptors and thus is affected by hormones. Ten percent of breast cancers are ER/progesterone receptor (PR)-positive and human epidermal growth factor receptor 2 (HER2)-positive, 69% are ER/PR-positive and HER2-negative, 7% are ER/PR-negative and HER2-positive, and the remaining 13% are classified as triple-negative [ 25 , 26 ]. Approximately 50% of breast cancers in postmenopausal women are ER-positive.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Estrogenic and Breast Cancer Inhibitory Activities of Chemical Constituents Isolated from Rheum undulatum L.

et al. 2018

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We investigated the estrogenic and breast cancer inhibitory activities of chemical constituents isolated from Rhei undulati Rhizoma (roots of Rheum undulatum L.), which is used as a laxative, an anti-inflammatory, and an anti-blood stagnation agent. Estrogen-like activity was studied using the well characterized E-screen assay in estrogen receptor (ER)-positive MCF-7 cells. The mechanism underlying the breast cancer inhibitory activity of the compounds was studied using human ER-negative MDA-MB-231 and ER-positive MCF-7 cells. The activation of apoptosis pathway-related proteins was investigated by western blotting, using extracts of R. undulatum prepared in three solvent conditions (EX1, EX2, and EX3). The R. undulatum chemical constituents (compounds 1–3) showed estrogen-like activity in the concentration range of 10 to 50 μM, by increasing the proliferation of human ER-positive MCF-7 cells. These effects were attenuated by co-treatment with 100 nM fulvestrant, an ER antagonist. Compounds 1–3 decreased the viability of MCF-7 cells in a concentration-dependent manner. Compounds 1 (aloe emodin) and 2 (rhapontigenin) induced mitochondria-independent apoptosis by activating the caspase-8 pathway, whereas the cytotoxic effect of compound 3 (chrysophanol 1-O-β-d-glucopyranoside) was mediated through the mitochondria-dependent apoptotic pathway.

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“…Animal groups were treated 60 min, before the test [ 29 ]. These doses were selected based on previous significant biologic findings of propolis extracts [ 30 , 31 , 32 ].…”

Section: Methodsmentioning

confidence: 99%

Effects of Green Brazilian Propolis Alcohol Extract on Nociceptive Pain Models in Rats

Al-Hariri

Abualait

2020

Plants

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Pain is one of the most common symptoms encountered in the medical practice. None of the management procedures used currently offer a complete relief for patients suffering from nociceptive pain. New treatment strategies for pain management are needed. Propolis has been used in traditional medicine to relieve various types of pain. The aim of the current study was to investigate the potential effects of the green Brazilian propolis alcohol extract in vivo on the nociceptive and inflammatory pain models in rats. Rats were distributed into three random groups (n = 6); Group I: control group received normal saline intraperitoneally (i.p.); Group II: treated with green Brazilian propolis alcohol extract (P50 mg/kg i.p.); Group III: treated with P100 mg/kg i.p. After sixty minutes, 50 μL of 5% formalin was injected subcutaneously into the dorsal surface of the right hind paw. The nociceptive response was identified by counting the number of flinches of the injected paw. The number of flinches was counted for the period of 0–5 min (early phase; neurogenic) and 10–60 min (late phase; inflammatory). Thermal hyperalgesia was assessed using three-paw withdrawal latency measurement with ten minutes intervals using a planter analgesic meter. Abdominal writhe (contraction) was induced by i.p. injection of acetic acid (1 mL of 2%). The results showed that green Brazilian propolis alcohol extract caused a significant inhibition of acetic acid-induced pain and significantly increased the pain threshold against infrared and formalin tests. The promising antinociceptive and anti-inflammatory properties of propolis and/or its active constituents as natural compounds in the present study indicates that it merits further studies in pain.

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Ethanol-extracted Cameroonian propolis exerts estrogenic effects and alleviates hot flushes in ovariectomized Wistar rats

Cited by 31 publications

References 47 publications

Chinese Propolis Prevents Obesity and Metabolism Syndromes Induced by a High Fat Diet and Accompanied by an Altered Gut Microbiota Structure in Mice

Chinese Propolis Prevents Obesity and Metabolism Syndromes Induced by a High Fat Diet and Accompanied by an Altered Gut Microbiota Structure in Mice

In Vitro Estrogenic and Breast Cancer Inhibitory Activities of Chemical Constituents Isolated from Rheum undulatum L.

Effects of Green Brazilian Propolis Alcohol Extract on Nociceptive Pain Models in Rats

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