Ethanol formed from arabinose: a rapid method for detecting Escherichia coli.

Coloe, Peter J.

doi:10.1136/jcp.31.4.361

Cited by 8 publications

(3 citation statements)

References 6 publications

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“…One drawback is the inability to tell apart Klebsiella aerogenes from E. coli, since both species ferment lactose to ethanol. In a similar study, 49 strains of E. coli were grown in media supplemented with arabinose [80]. It was confirmed that the bacteria produce ethanol with a maximum concentration achieved after eight hours.…”

Section: Detection Of Urinary Tract Infection Using Volatile Organic mentioning

confidence: 71%

Sniffing Out Urinary Tract Infection—Diagnosis Based on Volatile Organic Compounds and Smell Profile

2020

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Current available methods for the clinical diagnosis of urinary tract infection (UTI) rely on a urine dipstick test or culturing of pathogens. The dipstick test is rapid (available in 1–2 min), but has a low positive predictive value, while culturing is time-consuming and delays diagnosis (24–72 h between sample collection and pathogen identification). Due to this delay, broad-spectrum antibiotics are often prescribed immediately. The over-prescription of antibiotics should be limited, in order to prevent the development of antimicrobial resistance. As a result, there is a growing need for alternative diagnostic tools. This paper reviews applications of chemical-analysis instruments, such as gas chromatography–mass spectrometry (GC-MS), selected ion flow tube mass spectrometry (SIFT-MS), ion mobility spectrometry (IMS), field asymmetric ion mobility spectrometry (FAIMS) and electronic noses (eNoses) used for the diagnosis of UTI. These methods analyse volatile organic compounds (VOCs) that emanate from the headspace of collected urine samples to identify the bacterial pathogen and even determine the causative agent’s resistance to different antibiotics. There is great potential for these technologies to gain wide-spread and routine use in clinical settings, since the analysis can be automated, and test results can be available within minutes after sample collection. This could significantly reduce the necessity to prescribe broad-spectrum antibiotics and allow the faster and more effective use of narrow-spectrum antibiotics.

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Section: Detection Of Urinary Tract Infection Using Volatile Organic mentioning

confidence: 71%

Sniffing Out Urinary Tract Infection—Diagnosis Based on Volatile Organic Compounds and Smell Profile

2020

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“…The suspension was transferred to a 28-ml screw-capped bottle and incubated with shaking at 370C for four hours. The resulting culture was examined for ethanol and dimethyl disulphide by means of head-space gasliquid chromatography as described by Coloe (1978).…”

Section: Methodsmentioning

confidence: 99%

“…More recent culture methods such as the use of dip-slides (Craig and Kunin, 1972) do show the number of organisms present, but they are only marginally faster than routine culture methods and cannot be called rapid. This paper describes a rapid way of quantifying Escherichia coli and Proteus mirabilis in urine by a method based on those of Coloe (1978) and Hayward et al (1977).…”

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confidence: 99%

Head-space gas liquid chromatography for rapid detection of Escherichia coli and Proteus mirabilis in urine.

Coloe¹

1978

J Clin Pathol

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SUMMARY Measuring ethanol formed from arabinose by Escherichia coli and dimethyl disulphide formed from methionine by Proteus mirabilis is a rapid way of detecting these organisms in the urine. Ethanol and dimethyl disulphide are detected by head-space gas-liquid chromatography. Since sedimented organisms and not whole urine are used the method is not subject to interference from compounds within the urine. When 12 October 1977 that all were midstream specimens. Only the predominant organism was studied in detail provided it was well in excess of the contaminants that were often present.To sediment all the organisms 60 ml urine was centrifuged at 1000 g in a swing-out-bucket rotor of a Mistral 4L centrifuge for 10 minutes at 4VC. The supernatant was discarded and the sediment resuspended in 6 ml yeast-extract peptone water enriched with arabinose and methionine. The suspension was transferred to a 28-ml screw-capped bottle and incubated with shaking at 370C for four hours. The resulting culture was examined for ethanol and dimethyl disulphide by means of head-space gasliquid chromatography as described by Coloe (1978).Ethanol and dimethyl disulphide produced from bacterial cultures were measured by comparing the retention times of peaks with peaks from standard aqueous solutions of ethanol and dimethyl disulphide. They were confirmed as ethanol and dimethyl disulphide by mass spectrometery. Yields of ethanol and dimethyl disulphide were expressed as peak area in cm2, the area under the peak on the gas-liquid chromatograph chart.Peptone water was prepared from Bacteriological Peptone (Oxoid) as described by Cowan and Steel (1965). Peptone water enriched with 0-3 % yeast extract, 0 05 M arabinose (BDH), and 0-1 M methionine (BDH) was prepared in 100 ml lots, adjusted to pH 7T4, and sterilised by filtration through a sintered 365 on 11 May 2018 by guest. Protected by copyright.

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Analysis of Volatile Metabolites in Identification of Microbes and Diagnosis of Infectious Diseases

Larsson

Mårdh

Odham

1984

Gas Chromatography Mass Spectrometry Applications in Microbiology

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Ethanol formed from arabinose: a rapid method for detecting Escherichia coli.

Cited by 8 publications

References 6 publications

Sniffing Out Urinary Tract Infection—Diagnosis Based on Volatile Organic Compounds and Smell Profile

Sniffing Out Urinary Tract Infection—Diagnosis Based on Volatile Organic Compounds and Smell Profile

Head-space gas liquid chromatography for rapid detection of Escherichia coli and Proteus mirabilis in urine.

Analysis of Volatile Metabolites in Identification of Microbes and Diagnosis of Infectious Diseases

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