Ethanol increases HSP70 concentrations in honeybee (Apis mellifera L.) brain tissue

Hranıtz, John M.; Abramson, Charles I.; Carter, Richard P.

doi:10.1016/j.alcohol.2010.02.003

Cited by 45 publications

(24 citation statements)

References 49 publications

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“…HSP70 induction in the honey bee brain after ethanol exposure has been reported. HSP70 is induced by stress and is thought to function as a chaperone (Hranitz, Abramson, & Carter, 2010). In C. elegans, chronic ethanol exposure leads to an induction of a cytochrome p450 family of genes that are highly inducible by a wide range of environmental toxins (Peltonen, Aarnio, Heikkinen, Lakso, & Wong, 2013).…”

Section: Gene and Behaviormentioning

confidence: 99%

The Genetics of Alcohol Responses of Invertebrate Model Systems

Rothenfluh

Troutwine

Ghezzi

et al. 2014

Neurobiology of Alcohol Dependence

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Section: Gene and Behaviormentioning

confidence: 99%

The Genetics of Alcohol Responses of Invertebrate Model Systems

Rothenfluh

Troutwine

Ghezzi

et al. 2014

Neurobiology of Alcohol Dependence

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“…For a specific example, ethanol is a cellular stressor, which in honeybees leads to impaired behaviour and learning. Hranitz et al (2010) showed that ethanol stress leads to increased Hsp70 concentrations in honeybee brain tissue. Similarly, three unrelated stressors-diapause, heat stress and exposure to cold-induce increased expression of Hsps in the corn earworm, Helicoverpa zea (Zhang & Denlinger 2010).…”

Section: Induced Thermotolerancementioning

confidence: 99%

Molecular basis for insecticide‐enhanced thermotolerance in the brown planthopper Nilaparvata lugens Stål (Hemiptera:Delphacidae)

Huang

Gao

et al. 2013

Molecular Ecology

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Climate change is likely to have marked ecological effects on terrestrial ecosystems, including the activities of insect pests. Most attention has focused on the increasing geographical ranges of pests; however, if extrinsic factors enhance their thermotolerance, populations may express increased voltinism and longer daily and annual activity periods. These changes in pest populations have the potential for severe consequences, including increased crop losses and decreased food security at the global level. The brown planthopper (BPH) Nilaparvata lugens Stål (Hemiptera: Delphacidae) is a serious pest of rice crops in temperate and tropical regions of Asia. It is often present in rice microclimates at temperatures close to its maximum thermotolerance. Recent BPH outbreaks in tropical Asia are considered to be associated with excess use of pesticides and increasing temperature. This study tested whether exposure to sublethal concentrations of triazophos (tzp), an insecticide widely used in Asian rice production, enhances thermotolerance of BPH. Tzp exposure (40 ppm at 40 °C) significantly decreased mortality (from 94% in controls to 50% at 48 h post-treatment) and increased lethal mean time (LT50 ) of adults by 17.2 h. To investigate the underlying molecular mechanism of this tzp-enhanced thermotolerance, we selected Hsp70 and Arginine kinase (Argk) for detailed study. Transcripts encoding both proteins in third-instar nymphs and brachypterous adult females were up-regulated, compared with controls, after exposure to tzp. RNAi silencing of both genes demonstrated that Hsp70 and Argk are essential for survival and tzp-increased thermotolerance. We propose that tzp induces thermotolerance in BPHs by increasing the expression of genes that act in cell protection mechanisms. The significance of our proposal relates to the importance of understanding the influence of sublethal concentrations of insecticides on pest biology. In addition to its influence on thermotolerance, tzp also enhances BPH reproduction. We infer that exposure to a pesticide stressor can produce cross-tolerance, that is, increased tolerance to one stressor also increases tolerance to other stressors, including temperature. Aside from needing a better understanding of these effects in nature and in other pest/cropping systems, we suggest that pest management programmes can be improved with better understanding of the influences of stressors, including increased environmental temperatures and sublethal concentrations of insecticides, on pest biology.

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“…Blood EtOH concentrations rise immediately following consumption, peak after 30 minutes, and remain elevated for more than 4 hours postconsumption. Moreover, levels of HSP70 (heat shock proteins) in bees feed various EtOH solutions showed an inverted U‐shape curve of HSP70 concentration, indicating that ingesting 2.5% EtOH and 5% EtOH stimulated the stress response whereas ingestion of 10% EtOH inhibited the stress response (Hranitz et al., ).…”

mentioning

confidence: 99%

Ethanol Self‐Administration in Free‐Flying Honeybees (Apis mellifera L.) in an Operant Conditioning Protocol

Sokolowski

Abramson

Craig

2012

Alcoholism Clin & Exp Res

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Background: This study examines the effect of ethanol (EtOH) on continuous reinforcement schedules in the free-flying honeybee (Apis mellifera L.). As fermented nectars may be encountered naturally in the environment, we designed an experiment combining the tools of laboratory research with minimal disturbance to the natural life of honeybees.Methods: Twenty-five honeybees were trained to fly from their colonies to a fully automated operant chamber with head poking as the operant response. Load size, intervisit interval, and interresponse times (IRTs) served as the dependent variables and were monitored over the course of a daily training session consisting of many visits. Experimental bees were tested using an ABA design in which sucrose only was administered during condition A and a 5% EtOH sucrose solution was administered during condition B. Control bees received sucrose solution only.Results: Most bees continued to forage after EtOH introduction. EtOH significantly reduced the load size and the intervisit interval with no significant effect on IRTs. However, a look on individual data shows large individual differences suggesting the existence of different kinds of behavioral phenotypes linked to EtOH consumption and effects.Conclusions: Our results contribute to the study of EtOH consumption as a normal phenomenon in an ecological context and open the door to schedule-controlled drug self-administration studies in honeybees.

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Ethanol increases HSP70 concentrations in honeybee (Apis mellifera L.) brain tissue

Cited by 45 publications

References 49 publications

The Genetics of Alcohol Responses of Invertebrate Model Systems

The Genetics of Alcohol Responses of Invertebrate Model Systems

Molecular basis for insecticide‐enhanced thermotolerance in the brown planthopper Nilaparvata lugens Stål (Hemiptera:Delphacidae)

Ethanol Self‐Administration in Free‐Flying Honeybees (Apis mellifera L.) in an Operant Conditioning Protocol

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