Ethanol-induced cell agrwgation (flocculation) and its physiological background in schizosaccharomyces pombe rive 4-2-1

Maráz, Anna; Geleta, Anna

doi:10.1556/abiol.52.2001.2-3.7

Cited by 4 publications

(1 citation statement)

References 17 publications

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“…Nevertheless, the suggestion of a link to flocculation led us to investigate this possibility and to find that adhesion is specifically blocked by high concentrations of galactose, which also block flocculation in S. pombe (16,31). This suggests a role for galactose-binding adhesin proteins.…”

Section: Discussionmentioning

confidence: 99%

Functional Genomics of Adhesion, Invasion, and Mycelial Formation in Schizosaccharomyces pombe

et al. 2009

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Investigation into the switch between single-celled and filamentous forms of fungi may provide insights into cell polarity, differentiation, and fungal pathogenicity. At the molecular level, much of this investigation has fallen on two closely related budding yeasts, Candida albicans and Saccharomyces cerevisiae. Recently, the much more distant fission yeast Schizosaccharomyces pombe was shown to form invasive filaments after nitrogen limitation (E. Amoah-Buahin, N. Bone, and J. Armstrong, Eukaryot. Cell 4:1287-1297, 2005) and this genetically tractable organism provides an alternative system for the study of dimorphic growth. Here we describe a second mode of mycelial formation of S. pombe, on rich media. Screening of an S. pombe haploid deletion library identified 12 genes required for mycelial development which encode potential transcription factors, orthologues of S. cerevisiae Sec14p and Tlg2p, and the formin For3, among others. These were further grouped into two phenotypic classes representing different stages of the process. We show that galactose-dependent cell adhesion and actin assembly are both required for mycelial formation and mutants lacking a range of genes controlling cell polarity all produce mycelia but with radically altered morphology.Many fungi are capable of growth either as single-celled yeasts or in a variety of multicellular and invasive forms. The ability to switch between these forms may be related to the ability to undertake different phases of infection (28). Thus, the study of this switching process may provide not only an understanding of a basic process of eukaryotic differentiation but also an insight into mechanisms of pathogenicity.The fission yeast Schizosaccharomyces pombe is well established as a model eukaryote to study the cell cycle and control of polarized growth. It had widely been considered to exist only in a single-celled form. However, we discovered that under conditions of nitrogen limitation, S. pombe can form elaborate branched multicellular structures which deeply invade the growth medium, for which the cyclic AMP (cAMP) signaling pathway is required (2). Subsequently, it was shown that, as in Saccharomyces cerevisiae, a putative ammonium transporter is also required, presumably to sense the nitrogen concentration (18). In contrast to budding yeasts (25), invasion appeared to occur only within a very narrow range of conditions (2).Here we describe a second form of invasive growth by S. pombe which occurs on rich media. This allowed us to devise a simple screening of a collection of approximately 2,600 haploid deletion mutants for strains unable to invade the medium. This identified 12 genes associated with a range of functions, including transcriptional regulation, membrane trafficking, and control of actin assembly. Their roles in the invasion process could be further ordered into phases of adhesion to the surface and subsequent invasion of the medium. We investigated the role of specific sugars in this adhesion process and of the actin cytoskeleton in the fo...

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Section: Discussionmentioning

confidence: 99%

Functional Genomics of Adhesion, Invasion, and Mycelial Formation in Schizosaccharomyces pombe

et al. 2009

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2001

Yeast

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In order to keep subscribers up‐to‐date with the latest developments in their field, this current awareness service is provided by John Wiley & Sons and contains newly‐published material on yeasts. Each bibliography is divided into 10 sections. 1 Books, Reviews & Symposia; 2 General; 3 Biochemistry; 4 Biotechnology; 5 Cell Biology; 6 Gene Expression; 7 Genetics; 8 Physiology; 9 Medical Mycology; 10 Recombinant DNA Technology. Within each section, articles are listed in alphabetical order with respect to author. If, in the preceding period, no publications are located relevant to any one of these headings, that section will be omitted. (4 weeks journals ‐ search completed 5th. Sep. 2001)

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Disruption of ppr3, ppr4, ppr6 or ppr10 induces flocculation and filamentous growth in Schizosaccharomyces pombe

Chen

Huang

2018

FEMS Microbiology Letters

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Pentatricopeptide repeat (PPR) proteins are major players in mitochondrial and chloroplast RNA metabolism which is essential for normal organellar function. The fission yeast Schizosaccharomyces pombe has ten PPR proteins. We have previously reported that loss of ppr3, ppr4, ppr6, or ppr10 perturbs iron homeostasis leading to accumulation of reactive oxygen species and apoptotic cell death. In the present study, we show that loss of ppr3, ppr4, ppr6, or ppr10 can cause non-sexual flocculation and filamentous growth of cells. Furthermore, expression of a number of genes encoding cell-surface flocculins and cell wall-remodeling enzymes are induced in these ppr-deletion mutants. We also show that Δppr10 cells, and, to a lesser extent, Δppr4 and Δppr6 cells, exhibited increased tolerance to H2O2 toxicity compared with the wild-type strain. Finally, we found overexpression of genes involved in iron uptake and/or iron homeostasis could cause the flocculation of wild-type cells. Our findings suggest that elevated level of intracellular iron in the mutant caused by loss of ppr3, ppr4, ppr6, or ppr10 may result in flocculation and filamentous growth in S. pombe.

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Ethanol-induced cell agrwgation (flocculation) and its physiological background in schizosaccharomyces pombe rive 4-2-1

Cited by 4 publications

References 17 publications

Functional Genomics of Adhesion, Invasion, and Mycelial Formation in Schizosaccharomyces pombe

Functional Genomics of Adhesion, Invasion, and Mycelial Formation in Schizosaccharomyces pombe

Current Awareness

Disruption of ppr3, ppr4, ppr6 or ppr10 induces flocculation and filamentous growth in Schizosaccharomyces pombe

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