Ethanol-induced male infertility: Effects of aqueous leaf extract ofTetracarpidium conophorum

Abstract

This study investigated the effects of Tetracarpidium conophorum leaf extract on infertility induced by ethanol in male rats. Thirty rats were randomly divided into six groups of five animals each: Group 1 (positive control) received 0.9% saline only; Group 2 (ethanol alone) were given only 30% ethanol orally at 7 ml/kg body weight per day, thrice in a week; groups 3, 4 and 5 were given ethanol and co-treated with 50, 500 and 1,000 mg/kg body weight of leaf extract, respectively, while Group 6 was given ethanol and co-treated with a fertility drug, clomiphene citrate. Ethanol treatment resulted in significant (p < .05) decrease in LDH activity, G-6PDH activity, glycogen content, 3β and 17β HSD activities and testicular and epididymal Zn and Se contents and furthermore decrease in testicular sperm count, viability and marked increment in total sperm abnormalities, rate of sperm analysis parameters and consequently decreased reproductive hormone levels. Interestingly, co-administration of ethanol with either T. conophorum extract or drug almost ameliorated the toxic assault imposed by ethanol on reproductive organs and improved seminal qualities of the rats.

African star apple fruit pulp‐supplemented diet modulates fertility‐related biomolecules in the testis and epididymis of high‐fat diet/streptozotocin‐induced diabetic rats

“…Arginase plays an important role in the metabolism/regulation of arginine (Akomolafe et al, ). As the activity of arginase increases under the diabetic state, arginine is depleted and there is reduction in the synthesis of NO below physiological levels (Chang, Liao, & Kuo, ; Nikolic, Cvetkovic, & Sokolovic, ).…”

“…The epididymis was surgically removed and weighed. The epididymis was placed in physiological saline (0.9%) in the ratio of 1:5 weight (g) by volume (mL) (Akomolafe, Oboh, Akindahunsi, & Afolayan, ), and gently punctured using a sterile pin to release the sperm cells. The medium containing sperm cells was used for the estimation of the sperm parameters.…”

“…Epididymal sperm count (Pant & Srivastava, ), sperm progressive motility, and viability (Akomolafe et al, ; Yoshida et al, ) were carried out. Serum luteinizing hormone, follicle stimulating hormone, and testosterone levels were determined using enzyme‐linked immunosorbent assay (ELISA) using ELISA assay kits (AccuBind ELISA Microwell Monobind, Lake Forest, California, USA).…”

Protective role of Limonium bonduelli extract against non-enzymatic peroxidation in brain and testes induced by iron in vitro.

“…The increased lipid peroxidation in the presence of Fe 2+ could be attributed to the fact that Fe 2+ can catalyze one-electron transfer reactions that generate ROS such as reactive hydroxyl radical ( • OH) which is formed from H 2 O 2 through the Fenton reaction. Iron also decomposes lipid peroxides, thus generating peroxyl and alkoxyl radicals, which favors the propagation of lipid peroxidation [33,34] The natural phenolic compounds and flavonoids have received increasing interest in the last years They may reduce the risk of development of several diseases caused by oxidative stress, including neurodegenerative disorders [35] and testes injury [36,37]. Their protective effects stem for the ability to inhibit lipid peroxidation, chelat redox-active metals (by binding iron), and attenuate other processes involving reactive oxygen species (such as through radical scavenging) [38,39].…”

The influence of environmental factors on male fertility and its research progress

“…Drinking will also affect male fertility, the regulating effects of neuroendocrine gonadal axis, through direct effects on testis spermatogenic and hormone produced in the environment affected by the effects on sperm quality led to a decline in the number of hormone levels decreased by [5] . Studies have shown that the incidence of [6] male infertility is associated with heavy drinking, and alcohol consumption may be one of the principal causes of male infertility.…”