Ethanol-Induced Release of Adenosine 3′,5′-Monophosphate into the Medium by Rat Testis in Vitro*

Rajan, Valanila P.; Powell, Mary M.; Burgett, Amy C.; Bhalla, Vinod K.

doi:10.1210/endo-110-1-80

Cited by 10 publications

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“…We have shown that ethanol directly inhibits hCG stimulated testosterone production by rat isolated Leydig cells, results which are in agreement with those of Cicero et al (1980). In part, ethanol exerts its effects directly at the membrane level (Rajan et al -1982) and probably also at the level of the smooth endoplasmic reticulum (Murono et al -1980). Our results suggest that to study the direct effect of BR on testicular steroidogenesis, control incubation media should contain identical amounts of ethanol as incuba-tion with BR.…”

Section: Discussionmentioning

confidence: 89%

Bromocriptine, a Dopamine Agonist, Directly Inhibits Testosterone Production by Rat Leydig Cells

2009

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We investigated the direct effects of bromocriptine (BR) on both basal and hCG-stimulated testosterone production by rat collagenasedispersed Leydig cells. In a final volume of 2.2 ml, 2.106 Leydig cells were incubated at 33°C for 3 h either alone or with various amounts of hCG (1. 10.102. lo3. lo4 mUI/vial) and BR (1.5 lo-', 1.5 nificantly both basal and hCG-stimulated testosterone production whereas at lower doses, BR had nod effect.These results suggest that the dopamine itself may regulate rat Leydig cell function and that there is room for criticism of BR-induced hypoprolactinemia as an experimental model to study the effect of prolactin on the androgenic function. 1.5 lo-' M); BR was dissolved in 20 p1 of ethanol. BR (1.5 lo-' M) decreased sig-Bromocriptin, ein Dopaminagonist, hemmt direkt die Testosteronproduktion der Leydig-Zellen bei Ratten Zusammenfassung: Wir untersuchten die direkten Effekte des Bromocriptins (BR) sowohl auf die basale als auch auf die HCG-stimulierte Testosteronproduktion bei kollagenase-verteilten Leydig-Zellen von Ratten. In einem End-Volumen von 2,2 ml wurden 2 x 1 O6 Leydig-Zellen bei 33" C fur 3 Stunden entweder allein oder mit verschiedenen Mengen HCG (1,10, lo2, lo3, lo4 mUI/vial) und BR (1,s lo-', 1,s 1 3 lo-' M) inkubiert; BR wurde in 20 p1 Athanol gelost. BR (13 die basale als auch die HCC-stimulierte Testosteronproduktion, wohingegen niedrigere BR-Dosen keinen Effekt hatten. Ratte steuert und dai3 Raum ist fur eine Beurteilung der BR-induzierten Hypoprolaktinamie als einem experimentellen Modell, um die Effekte des Prolaktins auf die androgene Funktion zu untersuchen. M) senkte signifikant sowohl Diese Ergebnisse lassen vermuten, dafi das Dopamin selbst die Leydig-Zellfunktion der Introduction Bromocriptine (2-Br-alpha-ergocryptine mesylate) was specifically developped as an inhibitor of prolactin secretion; this action was explained by a direct stimulation of dopamine receptors (Clark et al. -1978).It decreases the testicular number of LH/hCG receptors, the testicular content and the In rats, treatment with bromocriptine (BR) induces impairment of testicular function.

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Section: Discussionmentioning

confidence: 89%