Ethanol induces autophagy regulated by mitochondrial ROS in Saccharomyces cerevisiae

Jing, Huaiqi; Liu, Huanhuan; Zhang, Lu; Gao, Jie; Song, Haoran; Tan, Xinqiu

doi:10.4014/jmb.1806.06014

Cited by 35 publications

(26 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results suggest that yeast could be activating its transcription and translation machinery to try to cope with cell death and autolysis. Jing et al [ 36 ] reported that the increase in the amount of ethanol in the medium induces the process of autophagy in the yeast during fermentation. Yeast autolysis involves the release of different products, resulting from the degradation of yeast macromolecules, into the wine.…”

Section: Resultsmentioning

confidence: 99%

Biological Processes Highlighted in Saccharomyces cerevisiae during the Sparkling Wines Elaboration

et al. 2020

View full text Add to dashboard Cite

Sparkling wines elaboration has been studied by several research groups, but this is the first report on analysis of biological processes according to the Gene Ontology terms (GO terms) and related to proteins expressed by yeast cells during the second fermentation of sparkling wines. This work provides a comprehensive study of the most relevant biological processes in Saccharomyces cerevisiae P29, a sparkling wine strain, during the second fermentation under two conditions (without and with endogenous CO2 overpressure) in the middle and the end of second fermentation. Consequently, a proteomic analysis with the OFFGEL fractionator and protein identification with LTQ Orbitrap XL coupled to HPLC were performed. The classification of biological processes was carried out using the tools provided by the Saccharomyces Genome Database. Results indicate that a greater number of biological processes were identified under condition without CO2 overpressure and in the middle of the fermentation versus the end of the second fermentation. The biological processes highlighted under condition without CO2 overpressure in the middle of the fermentation were involved in the carbohydrate and lipid metabolic processes and catabolic and biosynthetic processes. However, under CO2 overpressure, specific protein expression in response to stress, transport, translation, and chromosome organization and specific processes were not found. At the end of fermentation, there were higher specific processes under condition without CO2 overpressure; most were related to cell division, growth, biosynthetic process, and gene transcription resulting in increased cell viability in this condition. Under CO2 overpressure condition, the most representative processes were related to translation as tRNA metabolic process, chromosome organization, mRNA processing, ribosome biogenesis, and ribonucleoprotein complex assembly, probably in response to the stress caused by the hard fermentation conditions. Therefore, a broader knowledge of the adaptation of the yeast, and its behavior under typical conditions to produce sparkling wine, might improve and favor the wine industry and the selection of yeast for obtaining a high-quality wine.

show abstract

Section: Resultsmentioning

confidence: 99%

Biological Processes Highlighted in Saccharomyces cerevisiae during the Sparkling Wines Elaboration

et al. 2020

View full text Add to dashboard Cite

show abstract

“…In S. cerevisie, activity of cysteine protease Atg4 could be regulated by the redox state and, hence, it may regulate autophagosome formation [65]. Recently, ethanol stress-induced autophagy was reported to also be regulated by ROS [66].…”

Section: Discussionmentioning

confidence: 99%

Mmi1, the Yeast Ortholog of Mammalian Translationally Controlled Tumor Protein (TCTP), Negatively Affects Rapamycin-Induced Autophagy in Post-Diauxic Growth Phase

Vojtová¹,

Hašek²

2020

Cells

View full text Add to dashboard Cite

Translationally controlled tumor protein (TCTP) is a multifunctional and highly conserved protein from yeast to humans. Recently, its role in non-selective autophagy has been reported with controversial results in mammalian and human cells. Herein we examine the effect of Mmi1, the yeast ortholog of TCTP, on non-selective autophagy in budding yeast Saccharomyces cerevisiae, a well-established model system to monitor autophagy. We induced autophagy by nitrogen starvation or rapamycin addition and measured autophagy by using the Pho8Δ60 and GFP-Atg8 processing assays in WT, mmi1Δ, and in autophagy-deficient strains atg8Δ or atg1Δ. Our results demonstrate that Mmi1 does not affect basal or nitrogen starvation-induced autophagy. However, an increased rapamycin-induced autophagy is detected in mmi1Δ strain when the cells enter the post-diauxic growth phase, and this phenotype can be rescued by inserted wild-type MMI1 gene. Further, the mmi1Δ cells exhibit significantly lower amounts of reactive oxygen species (ROS) in the post-diauxic growth phase compared to WT cells. In summary, our study suggests that Mmi1 negatively affects rapamycin-induced autophagy in the post-diauxic growth phase and supports the role of Mmi1/TCTP as a negative autophagy regulator in eukaryotic cells.

show abstract

“…The deletion of ATG1 in Candida glabrata results in higher intracellular ROS than the WT, especially upon induction by H 2 O 2 (Shimamura et al, 2019). Ethanol stress can also induce autophagy, which is regulated by H 2 O 2 and superoxide anion (O 2 − ) derived from the mitochondrial electron transport chain (mtETC), and autophagy in turn contributes to the elimination of H 2 O 2 and O 2 − (Jing et al, 2018). In C. neoformans, whether this relationship exists between ROS elimination and autophagy is worth exploring.…”

Section: Discussionmentioning

confidence: 99%

Conserved Autophagy Pathway Contributes to Stress Tolerance and Virulence and Differentially Controls Autophagic Flux Upon Nutrient Starvation in Cryptococcus neoformans

et al. 2019

View full text Add to dashboard Cite

Autophagy is mainly a catabolic process, which is used to cope with nutrient deficiency and various stress conditions. Human environment often imposes various stresses on Cryptococcus neoformans, a major fungal pathogen of immunocompromised individuals; therefore, autophagic response of C. neoformans to these stresses often determines its survival in the host. However, a systematic study on how autophagy related (ATG) genes influence on autophagic flux, virulence, stress response and pathogenicity of C. neoformans is lacking. In this study, 22 ATG-deficient strains were constructed to investigate their roles in virulence, pathogenesis, stress response, starvation tolerance and autophagic flux in C. neoformans. Our results showed that Atg6 and Atg14-03 significantly affect the growth of C. neoformans at 37 • C and laccase production. Additionally, atg2 and atg6 strains were sensitive to oxidative stress caused by hydrogen peroxide. Approximately half of the atg strains displayed higher sensitivity to 1.5 M NaCl and remarkably lower virulence in the Galleria mellonella model than the wild type. Autophagic flux in C. neoformans was dependent on the Atg1-Atg13, Atg5-Atg12-Atg16, and Atg2-Atg18 complexes and Atg11. Cleavage of the green fluorescent protein (GFP) from Atg8 was difficult to detect in these autophagy defective mutants; however, it was detected in the atg3 , atg4 , atg6 and atg14 strains. Additionally, no homologs of Saccharomyces cerevisiae ATG10 were detected in C. neoformans. Our results indicate that these ATG genes contribute differentially to carbon and nitrogen starvation tolerance in C. neoformans compared with S. cerevisiae. Overall, this study advances our knowledge of the specific roles of ATG genes in C. neoformans.

show abstract

Ethanol induces autophagy regulated by mitochondrial ROS in Saccharomyces cerevisiae

Cited by 35 publications

References 46 publications

Biological Processes Highlighted in Saccharomyces cerevisiae during the Sparkling Wines Elaboration

Biological Processes Highlighted in Saccharomyces cerevisiae during the Sparkling Wines Elaboration

Mmi1, the Yeast Ortholog of Mammalian Translationally Controlled Tumor Protein (TCTP), Negatively Affects Rapamycin-Induced Autophagy in Post-Diauxic Growth Phase

Conserved Autophagy Pathway Contributes to Stress Tolerance and Virulence and Differentially Controls Autophagic Flux Upon Nutrient Starvation in Cryptococcus neoformans

Contact Info

Product

Resources

About