Ethanol induces heat tolerance in plants by stimulating unfolded protein response

Matsui, Atsushi; Todaka, Daisuke; Tanaka, Maho; Mizunashi, Kayoko; Takahashi, Satoshi; Sunaoshi, Yuji; Tsuboi, Yuuri; Ishida, Junko; Kikuchi, Jun; Kusano, Miyako; Kawaura, Kanako; Seki, Motoaki

doi:10.1007/s11103-022-01291-8

Cited by 11 publications

(15 citation statements)

References 83 publications

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“…The acetic acid treatment hinders seed germination and plant growth on Murashige and Skoog (MS) medium as compared to the ethanol treatment (Supplementary Fig. S13; Matsui et al 2022). Moreover, in soil a prolonged application of acetic acid considerably decreased the plant growth as compared to the ethanol-treated plants (Supplementary Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Acetic acid considerably hinders seed germination and plant growth in A. thaliana at concentrations as low as 1 mM (Supplementary Fig. S13), whereas ethanol does not affect seed germination and plant growth in A. thaliana up to the concentrations of 15 mM (Matsui et al 2022). We also tested if the prolonged application of ethanol and acetic acid affects plant growth.…”

Section: Involvement Of Gluconeogenesis and Acetic Acid Biosynthesis ...mentioning

confidence: 99%

“…The exogenous application of plant metabolites reportedly enhances plant stress tolerance (Sako et al 2021). Acetic acid increases drought tolerance, while ethanol can mitigate the detrimental effects of salinity and heat stress (Kim et al 2017, Nguyen et al 2017, Ogawa et al 2021, Sako et al 2021, Matsui et al 2022. In plants, ethanol fermentation ensures that energy is conserved under stress conditions (Tadege et al 1999).…”

Section: Introductionmentioning

confidence: 99%

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Ethanol-Mediated Novel Survival Strategy against Drought Stress in Plants

Bashir

Todaka

Rasheed

et al. 2022

Plant and Cell Physiology

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Water scarcity is a serious agricultural problem causing significant losses to crop yield and product quality. The development of technologies to mitigate the damage caused by drought stress is essential for ensuring a sustainable food supply for the increasing global population. We herein report that the exogenous application of ethanol, an inexpensive and environmentally friendly chemical, significantly enhances drought tolerance in Arabidopsis thaliana, rice and wheat. The transcriptomic analyses of ethanol-treated plants revealed the upregulation of genes related to sucrose and starch metabolism, phenylpropanoids and glucosinolate biosynthesis, while metabolomic analysis showed an increased accumulation of sugars, glucosinolates and drought-tolerance-related amino acids. The phenotyping analysis indicated that drought-induced water loss was delayed in the ethanol-treated plants. Furthermore, ethanol treatment induced stomatal closure, resulting in decreased transpiration rate and increased leaf water contents under drought stress conditions. The ethanol treatment did not enhance drought tolerance in the mutant of ABI1, a negative regulator of abscisic acid (ABA) signaling in Arabidopsis, indicating that ABA signaling contributes to ethanol-mediated drought tolerance. The nuclear magnetic resonance analysis using 13C-labeled ethanol indicated that gluconeogenesis is involved in the accumulation of sugars. The ethanol treatment did not enhance the drought tolerance in the aldehyde dehydrogenase (aldh) triple mutant (aldh2b4/aldh2b7/aldh2c4). These results show that ABA signaling and acetic acid biosynthesis are involved in ethanol-mediated drought tolerance and that chemical priming through ethanol application regulates sugar accumulation and gluconeogenesis, leading to enhanced drought tolerance and sustained plant growth. These findings highlight a new survival strategy for increasing crop production under water-limited conditions.

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Section: Discussionmentioning

confidence: 99%

Section: Involvement Of Gluconeogenesis and Acetic Acid Biosynthesis ...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ethanol-Mediated Novel Survival Strategy against Drought Stress in Plants

Bashir

Todaka

Rasheed

et al. 2022

Plant and Cell Physiology

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“…Ethanol induces the sprouting of potato tubers [32], fastens bud burst of grapevine [33], breaks seed dormancy, and stimulates germination in rice [34]. Further studies showed that ethanol can increase plant tolerance to abiotic factors such as drought stress by keeping stomata closed [35], heat stress by stimulating unfolded protein response [36], saline stress by detoxifying ROS [24], or high‐light stress by suppressing ROS accumulation [37]. Exogenous application of ethanol on vines produced berries with higher pigment concentration [38], increased storage time of table grapes without altering taste [39], increased fructose, sucrose, and other volatiles of interest in cherry tomato fruit and improved sensory quality of the fruits, redder and riper than controls [40].…”

Section: Introduction: Ethanol a Metabolite With Controversial Rolesmentioning

confidence: 99%

Responses of animals and plants to physiological doses of ethanol: a molecular messenger of hypoxia?

Diot,

Groth,

Blanchet

et al. 2024

The FEBS Journal

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Our viewpoint is that ethanol could act as a molecular messenger in animal and plant organisms under conditions of hypoxia or other stresses and could elicit physiological responses to such conditions. There is evidence that both animal and plant organisms have endogenous levels of ethanol, but reports on the changes induced by this alcohol at physiological levels are sparse. Studies have shown that ethanol has different effects on cell metabolism at low and high concentrations, resembling a hormetic response. Further studies have addressed the potential cellular and molecular mechanisms used by organisms to sense changes in physiological concentrations of ethanol. This article summarizes the possible mechanisms by which ethanol may be sensed, particularly at the cell membrane level. Our analysis shows that current knowledge on this subject is limited. More research is required on the effects of ethanol at very low doses, in plants and animals at both molecular and physiological levels. We believe that further research on this topic could lead to new discoveries in physiology and may even help us understand metabolic adjustments related to climate change. As temperatures rise more frequently, dissolved oxygen levels drop, leading to hypoxic conditions and consequently, an increase in cellular ethanol levels.

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“…Exogenous treatment with acetic acid increases the expression levels of jasmonate signalling pathway genes, conferring tolerance to drought stress in several plant species, such as Arabidopsis thaliana (Arabidopsis), Zea mays , Triticum aestivum , Oryza sativa (rice), and Brassica napus ( Kim et al., 2017 ). Arabidopsis and Lactuca sativa plants treated with ethanol exhibit enhanced tolerance to heat stress, which is associated with the stimulation of response to unfolded proteins ( Matsui et al., 2022 ). Heat stress is also modulated following treatment with β-aminobutyric acid (BABA) in Arabidopsis and Brassica rapa , through the induction of heat shock proteins and the improvement of photosynthesis performance and antioxidant systems, respectively ( Zimmerli et al., 2008 ; Quan et al., 2022 ).…”

Section: Introductionmentioning

confidence: 99%

N-acetylglutamic acid alleviates oxidative stress based on histone acetylation in plants

2023

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Oxidative stress causes cellular damage and genomic instability through the accumulation of reactive oxygen species (ROS) in plants, resulting in reduced crop production. Chemical priming, which can enhance plant tolerance to environmental stress using functional chemical compounds, is expected to improve agricultural yield in various plants without genetic engineering. In the present study, we revealed that non-proteogenic amino acid N-acetylglutamic acid (NAG) can alleviate oxidative stress damage in Arabidopsis thaliana (Arabidopsis) and Oryza sativa (rice). Exogenous treatment with NAG prevented chlorophyll reduction induced by oxidative stress. The expression levels of ZAT10 and ZAT12, which are regarded as master transcriptional regulators in response to oxidative stress, increased following NAG treatment. Additionally, Arabidopsis plants treated with NAG showed enhanced levels of histone H4 acetylation at ZAT10 and ZAT12 with the induction of histone acetyltransferases HAC1 and HAC12. The results suggest that NAG could enhance tolerance to oxidative stress through epigenetic modifications and contribute to the improvement of crop production in a wide variety of plants under environmental stress.

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Ethanol induces heat tolerance in plants by stimulating unfolded protein response

Cited by 11 publications

References 83 publications

Ethanol-Mediated Novel Survival Strategy against Drought Stress in Plants

Ethanol-Mediated Novel Survival Strategy against Drought Stress in Plants

Responses of animals and plants to physiological doses of ethanol: a molecular messenger of hypoxia?

N-acetylglutamic acid alleviates oxidative stress based on histone acetylation in plants

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