Ethanol inhibits human bone cell proliferation and function in vitro

Friday, Karen E.; Howard, Guy A.

doi:10.1016/0026-0495(91)90044-w

Cited by 118 publications

(61 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This difference might be mediated through VDR gene polymorphism. A dose-dependent effect of alcohol on bone metabolism has also been reported (29), but the precise influence of moderate alcohol con-sumption on bone metabolism is still unknown. A recent meta-analysis indicated that men and women who consume more than 2 drinks per day have a higher risk of hip fracture (30).…”

Section: Discussionmentioning

confidence: 99%

Relation of Bone Mineral Density to Vitamin D Receptor Gene Polymorphism and Lifestyle Factors in Japanese Female Workers Aged 22-44 Years: A Cross-Sectional Study

Funakoshi

Omori

Katoh

2010

J Nutr Sci Vitaminol

View full text Add to dashboard Cite

Summary Bone mineral density (BMD) reflects both genetic and lifestyle factors. The aim of this cross-sectional study was to investigate the influence of vitamin D receptor ( VDR ) gene polymorphism and lifestyle factors on BMD in premenopausal female workers. The subjects were 162 premenopausal female employees aged 22-44 y who worked at a large-scale integrated manufacturing facility in Japan. BMD was measured at the nondominant radius by dual energy X-ray absorptiometry. Lifestyle information was obtained by a questionnaire at the same time and genomic DNA was isolated from peripheral leukocytes. BMD was positively correlated with age, weight, and body mass index (BMI). The genotype frequencies of VDR gene polymorphism detected by Taq I analysis were 77.2, 22.8, and 0.0% for TT , Tt , and tt , respectively. Analysis of covariance with adjustment for age and BMI showed that the mean BMD was significantly lower in subjects with the Tt genotype than in those with the TT genotype. Subjects who drank alcohol also showed a significantly lower BMD if they had the Tt genotype than if they had the TT genotype. According to multiple linear regression analysis, the independent determinants of BMD were age, BMI, and VDR gene polymorphism. Our data show that BMD is negatively correlated with the Tt genotype of the VDR gene, but positively correlated with age and BMI. These findings suggest that analysis of VDR gene polymorphism may be useful for identifying individuals who are susceptible to osteoporosis so that early preventive measures can be provided. Key Words bone mineral density, gene polymorphism, lifestyle factors, vitamin D receptor In Japan, prevention of osteoporosis is a high-priority issue because osteoporotic fracture is one of the primary causes of elderly persons becoming bedridden ( 1 ). Measurement of bone mineral density (BMD) is the best tool available to assess osteoporotic fracture risk, since low BMD is an important risk factor for osteoporotic fracture ( 2 ). The peak BMD of Japanese women is reached around 35-40 y of age, and it falls rapidly during the first 10 y after menopause and then decreases gradually ( 3 ). Because a decrease of BMD is inevitable after menopause, it is necessary to identify and manage any premenopausal decrease of BMD. BMD can be influenced by various lifestyle factors, such as smoking, alcohol intake, exercise, and nutrition ( 4 ). Among the genes that may be associated with the development of osteoporosis, polymorphism of the vitamin D receptor ( VDR ) gene has been most widely studied ( 5 ), and a meta-analysis ( 6 ) suggested that BMD is influenced by VDR gene polymorphism. BMD is affected by both genetic and lifestyle factors ( 5 ), but most studies have separately investigated the effects of VDR gene polymorphism or lifestyle factors on BMD. Better understanding of this combined effect may allow us to identify a highrisk group for osteoporosis and lead to more effective osteoporosis prevention and to improvement of public health. A few previous studies ( 7-10 ) have ...

show abstract

Section: Discussionmentioning

confidence: 99%

Relation of Bone Mineral Density to Vitamin D Receptor Gene Polymorphism and Lifestyle Factors in Japanese Female Workers Aged 22-44 Years: A Cross-Sectional Study

Funakoshi

Omori

Katoh

2010

J Nutr Sci Vitaminol

View full text Add to dashboard Cite

show abstract

“…Depression is associated with smoking [52], which is associated with lower BMD by inhibiting estrogen activity and inhibiting calcium absorption by the intestines [53]. Depression is also associated with increased alcohol use [54], and chronic alcohol use is also associated with low BMD by inhibition of bone cell proliferation and function [55,56]. Depression is associated with fatigue and physical inactivity [57,58], and physical activity is associated with increased BMD [36].…”

Section: Behaviorsmentioning

confidence: 99%

Depression and osteoporosis: epidemiology and potential mediating pathways

2007

View full text Add to dashboard Cite

Introduction-There have been numerous studies examining the association between depression and bone mineral density (BMD), but the underlying nature of this relationship remains unclear. Independent of this association, there is a growing body of evidence that depression impacts the risk for fracture in older adults. This article reviews the current epidemiological evidence regarding comorbidity of depression, low bone mineral density, and fracture.

show abstract

“…After osteoclasts are differentiated, their activity is regulated by a variety of hormones, growth factors, and cytokines (Teitelbaum, 2000). Alcohol abuse may promote bone loss through both inhibition of osteoblastogenesis (Friday and Howard, 1991) and induction of osteoclast differentiation and activation (Cheung et al, 1995). Dai et al (2000) suggested that EtOH increases RANKL mRNA expression in bone marrow cells resulting in stimulation of osteoclastogenesis and bone resorption mediated via induction of IL-6 (Dai et al, 2000).…”

mentioning

confidence: 99%

Estradiol Protects against Ethanol-Induced Bone Loss by Inhibiting Up-Regulation of Receptor Activator of Nuclear Factor-κB Ligand in Osteoblasts

Chen

Haley

Hidestrand

et al. 2006

J Pharmacol Exp Ther

View full text Add to dashboard Cite

To investigate the effects of sex hormones on ethanol (EtOH)-induced bone loss, female Sprague-Dawley rats were fed control or EtOH-containing diets (12 g/kg/day) by intragastric infusion. After 3 weeks, rats receiving EtOH had significant decreases in tibial trabecular and total bone mineral density, induction of receptor activator of nuclear factor-〉 ligand (RANKL) mRNA expression, and enhanced bone resorption, all of which were prevented by treatment with 17␤-estradiol (E 2 ). The addition of progesterone did not enhance the beneficial effect of E 2 alone. Consistent with our in vivo findings, EtOH stimulated RANKL mRNA expression in cultured primary osteoblasts, and this expression was blocked by 4-methylpyrazole. Acetaldehyde also induced RANKL expression. Class 1 alcohol dehydrogenase was found to be expressed and EtOHinducible in cultured osteoblasts, whereas CYP2E1 was undetectable. We found that EtOH induced phosphorylation of extracellular signal-regulated kinase (ERK) and signal transducers and activators of transcription 3 (STAT3). E 2 and the mitogenactivated protein kinase kinase inhibitor 2Ј-amino-3Ј-methoxyflavone (PD98059) blocked ERK and STAT3 phosphorylation and blocked RANKL induction. Moreover, E 2 completely blocked EtOH-induced osteoclastogenesis in a primary osteoblast and osteoclast precursor coculture system. The E 2 effects were estrogen receptor-mediated. Therefore, E 2 prevents EtOH-induced bone loss by opposing the induction of RANKL mRNA in osteoblasts and ethanol-induced osteoclastogenesis, through opposing effects on sustained ERK signaling.

show abstract

Ethanol inhibits human bone cell proliferation and function in vitro

Cited by 118 publications

References 10 publications

Relation of Bone Mineral Density to Vitamin D Receptor Gene Polymorphism and Lifestyle Factors in Japanese Female Workers Aged 22-44 Years: A Cross-Sectional Study

Relation of Bone Mineral Density to Vitamin D Receptor Gene Polymorphism and Lifestyle Factors in Japanese Female Workers Aged 22-44 Years: A Cross-Sectional Study

Depression and osteoporosis: epidemiology and potential mediating pathways

Estradiol Protects against Ethanol-Induced Bone Loss by Inhibiting Up-Regulation of Receptor Activator of Nuclear Factor-κB Ligand in Osteoblasts

Contact Info

Product

Resources

About